Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancer
Abstract Photothermal therapy is favored by cancer researchers due to its advantages such as controllable initiation, direct killing and immune promotion. However, the low enrichment efficiency of photosensitizer in tumor site and the limited effect of single use limits the further development of ph...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-024-02417-4 |
| _version_ | 1797209077026127872 |
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| author | Zhou Lan Wei-Jia Liu Wu-Wei Yin Sheng-Ren Yang Hao Cui Ke-Long Zou Guo-Wang Cheng Hao Chen Yan-Hua Han Lang Rao Rui Tian Ling-Ling Li Yu-Yue Zhao Guang-Tao Yu |
| author_facet | Zhou Lan Wei-Jia Liu Wu-Wei Yin Sheng-Ren Yang Hao Cui Ke-Long Zou Guo-Wang Cheng Hao Chen Yan-Hua Han Lang Rao Rui Tian Ling-Ling Li Yu-Yue Zhao Guang-Tao Yu |
| author_sort | Zhou Lan |
| collection | DOAJ |
| description | Abstract Photothermal therapy is favored by cancer researchers due to its advantages such as controllable initiation, direct killing and immune promotion. However, the low enrichment efficiency of photosensitizer in tumor site and the limited effect of single use limits the further development of photothermal therapy. Herein, a photo-responsive multifunctional nanosystem was designed for cancer therapy, in which myeloid-derived suppressor cell (MDSC) membrane vesicle encapsulated decitabine-loaded black phosphorous (BP) nanosheets (BP@ Decitabine @MDSCs, named BDM). The BDM demonstrated excellent biosafety and biochemical characteristics, providing a suitable microenvironment for cancer cell killing. First, the BDM achieves the ability to be highly enriched at tumor sites by inheriting the ability of MDSCs to actively target tumor microenvironment. And then, BP nanosheets achieves hyperthermia and induces mitochondrial damage by its photothermal and photodynamic properties, which enhancing anti-tumor immunity mediated by immunogenic cell death (ICD). Meanwhile, intra-tumoral release of decitabine induced G2/M cell cycle arrest, further promoting tumor cell apoptosis. In vivo, the BMD showed significant inhibition of tumor growth with down-regulation of PCNA expression and increased expression of high mobility group B1 (HMGB1), calreticulin (CRT) and caspase 3. Flow cytometry revealed significantly decreased infiltration of MDSCs and M2-macrophages along with an increased proportion of CD4+, CD8+ T cells as well as CD103+ DCs, suggesting a potentiated anti-tumor immune response. In summary, BDM realizes photothermal therapy/photodynamic therapy synergized chemotherapy for cancer. |
| first_indexed | 2024-04-24T09:48:57Z |
| format | Article |
| id | doaj.art-915f5ee679b8443ba7be503e51d52683 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-24T09:48:57Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-915f5ee679b8443ba7be503e51d526832024-04-14T11:28:19ZengBMCJournal of Nanobiotechnology1477-31552024-04-0122111610.1186/s12951-024-02417-4Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancerZhou Lan0Wei-Jia Liu1Wu-Wei Yin2Sheng-Ren Yang3Hao Cui4Ke-Long Zou5Guo-Wang Cheng6Hao Chen7Yan-Hua Han8Lang Rao9Rui Tian10Ling-Ling Li11Yu-Yue Zhao12Guang-Tao Yu13Stomatological Hospital, School of Stomatology, Southern Medical UniversityDepartment of Oral Mucosal Diseases, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Affiliated Stomatology Hospital of Guangzhou Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityScience and Technology Innovation Center, Guangzhou University of Chinese MedicineStomatological Hospital, School of Stomatology, Southern Medical UniversityScience and Technology Innovation Center, Guangzhou University of Chinese MedicineInstitute of Biomedical Health Technology and Engineering, Shenzhen Bay LaboratoryState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen UniversityDepartment of Pharmaceutics, School of Pharmacy, Nanjing Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityAbstract Photothermal therapy is favored by cancer researchers due to its advantages such as controllable initiation, direct killing and immune promotion. However, the low enrichment efficiency of photosensitizer in tumor site and the limited effect of single use limits the further development of photothermal therapy. Herein, a photo-responsive multifunctional nanosystem was designed for cancer therapy, in which myeloid-derived suppressor cell (MDSC) membrane vesicle encapsulated decitabine-loaded black phosphorous (BP) nanosheets (BP@ Decitabine @MDSCs, named BDM). The BDM demonstrated excellent biosafety and biochemical characteristics, providing a suitable microenvironment for cancer cell killing. First, the BDM achieves the ability to be highly enriched at tumor sites by inheriting the ability of MDSCs to actively target tumor microenvironment. And then, BP nanosheets achieves hyperthermia and induces mitochondrial damage by its photothermal and photodynamic properties, which enhancing anti-tumor immunity mediated by immunogenic cell death (ICD). Meanwhile, intra-tumoral release of decitabine induced G2/M cell cycle arrest, further promoting tumor cell apoptosis. In vivo, the BMD showed significant inhibition of tumor growth with down-regulation of PCNA expression and increased expression of high mobility group B1 (HMGB1), calreticulin (CRT) and caspase 3. Flow cytometry revealed significantly decreased infiltration of MDSCs and M2-macrophages along with an increased proportion of CD4+, CD8+ T cells as well as CD103+ DCs, suggesting a potentiated anti-tumor immune response. In summary, BDM realizes photothermal therapy/photodynamic therapy synergized chemotherapy for cancer.https://doi.org/10.1186/s12951-024-02417-4Oral squamous cell carcinomaBlack phosphorousDecitabineMDSCsPhotothermal therapyPhotodynamic therapy |
| spellingShingle | Zhou Lan Wei-Jia Liu Wu-Wei Yin Sheng-Ren Yang Hao Cui Ke-Long Zou Guo-Wang Cheng Hao Chen Yan-Hua Han Lang Rao Rui Tian Ling-Ling Li Yu-Yue Zhao Guang-Tao Yu Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancer Journal of Nanobiotechnology Oral squamous cell carcinoma Black phosphorous Decitabine MDSCs Photothermal therapy Photodynamic therapy |
| title | Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancer |
| title_full | Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancer |
| title_fullStr | Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancer |
| title_full_unstemmed | Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancer |
| title_short | Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancer |
| title_sort | biomimetic mdscs membrane coated black phosphorus nanosheets system for photothermal therapy photodynamic therapy synergized chemotherapy of cancer |
| topic | Oral squamous cell carcinoma Black phosphorous Decitabine MDSCs Photothermal therapy Photodynamic therapy |
| url | https://doi.org/10.1186/s12951-024-02417-4 |
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