The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating <i>Enterococcus faecalis</i> Biofilm from Infected Root Canals: An In Vitro Study
Objectives: This study aimed to assess the efficacy of shockwave-enhanced emission photoacoustic streaming (SWEEPS) plus antimicrobial photodynamic therapy (aPDT) using indocyanine green (ICG) for the elimination of <i>Enterococcus faecalis</i> biofilm from infected root canals. Material...
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MDPI AG
2023-06-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/7/1850 |
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| author | Golriz Rostami Shima Afrasiabi Stefano Benedicenti Antonio Signore Nasim Chiniforush |
| author_facet | Golriz Rostami Shima Afrasiabi Stefano Benedicenti Antonio Signore Nasim Chiniforush |
| author_sort | Golriz Rostami |
| collection | DOAJ |
| description | Objectives: This study aimed to assess the efficacy of shockwave-enhanced emission photoacoustic streaming (SWEEPS) plus antimicrobial photodynamic therapy (aPDT) using indocyanine green (ICG) for the elimination of <i>Enterococcus faecalis</i> biofilm from infected root canals. Materials and Methods: thirty sound human single-canal teeth were chosen and standardized to have 12 mm of root length. The root canals were shaped and prepared by means of ProTaper rotary files. After sterilization of the teeth, the canals were inoculated with <i>E. faecalis</i> for 2 weeks. The teeth were then randomly divided into six groups (n = five) of control, ICG, ICG + 808 nm diode laser, ICG + SWEEPS, ICG + 808 nm diode laser + SWEEPS, and 5.25% sodium hypochlorite (NaOCl). Following treatment, the number of colony-forming units (CFUs)/mL were calculated for each group. Statistical analysis was carried out using one-way ANOVA. For multiple comparisons, Tukey’s test was used as the post hoc test. Results: NaOCl alone showed the highest efficacy (<i>p</i> < 0.001). The ICG + 808 nm diode laser + SWEEPS group displayed significantly lower amounts of bacteria than either the ICG + 808 nm diode laser or SWEEPS (<i>p</i> < 0.001). There was a statistically significant difference detected between the ICG + 808 nm diode laser and ICG + SWEEPS (<i>p</i> = 0.035). Conclusions: SWEEPS can effectively increase the photosensitizer distribution in the root canal space, and its application along with irrigants can bring about promising results. |
| first_indexed | 2024-03-11T01:16:47Z |
| format | Article |
| id | doaj.art-9162363968f14d22b1ae76d0ed548dcf |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T01:16:47Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-9162363968f14d22b1ae76d0ed548dcf2023-11-18T18:25:52ZengMDPI AGBiomedicines2227-90592023-06-01117185010.3390/biomedicines11071850The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating <i>Enterococcus faecalis</i> Biofilm from Infected Root Canals: An In Vitro StudyGolriz Rostami0Shima Afrasiabi1Stefano Benedicenti2Antonio Signore3Nasim Chiniforush4Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran 1441987566, IranLaser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran 1441987566, IranDepartment of Surgical Sciences and Integrated Diagnostics, University of Genoa, Viale Benedetto XV 6, 16132 Genoa, ItalyTherapeutic Dentistry Department, Institute of Dentistry, I.M. Sechenov First Moscow State Medical University, Trubetskaya Str., 8, b. 2, 119992 Moscow, RussiaDepartment of Surgical Sciences and Integrated Diagnostics, University of Genoa, Viale Benedetto XV 6, 16132 Genoa, ItalyObjectives: This study aimed to assess the efficacy of shockwave-enhanced emission photoacoustic streaming (SWEEPS) plus antimicrobial photodynamic therapy (aPDT) using indocyanine green (ICG) for the elimination of <i>Enterococcus faecalis</i> biofilm from infected root canals. Materials and Methods: thirty sound human single-canal teeth were chosen and standardized to have 12 mm of root length. The root canals were shaped and prepared by means of ProTaper rotary files. After sterilization of the teeth, the canals were inoculated with <i>E. faecalis</i> for 2 weeks. The teeth were then randomly divided into six groups (n = five) of control, ICG, ICG + 808 nm diode laser, ICG + SWEEPS, ICG + 808 nm diode laser + SWEEPS, and 5.25% sodium hypochlorite (NaOCl). Following treatment, the number of colony-forming units (CFUs)/mL were calculated for each group. Statistical analysis was carried out using one-way ANOVA. For multiple comparisons, Tukey’s test was used as the post hoc test. Results: NaOCl alone showed the highest efficacy (<i>p</i> < 0.001). The ICG + 808 nm diode laser + SWEEPS group displayed significantly lower amounts of bacteria than either the ICG + 808 nm diode laser or SWEEPS (<i>p</i> < 0.001). There was a statistically significant difference detected between the ICG + 808 nm diode laser and ICG + SWEEPS (<i>p</i> = 0.035). Conclusions: SWEEPS can effectively increase the photosensitizer distribution in the root canal space, and its application along with irrigants can bring about promising results.https://www.mdpi.com/2227-9059/11/7/1850antimicrobial photodynamic therapybiofilmsdisinfectionEr:YAG laser<i>Enterococcus faecalis</i>indocyanine green |
| spellingShingle | Golriz Rostami Shima Afrasiabi Stefano Benedicenti Antonio Signore Nasim Chiniforush The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating <i>Enterococcus faecalis</i> Biofilm from Infected Root Canals: An In Vitro Study Biomedicines antimicrobial photodynamic therapy biofilms disinfection Er:YAG laser <i>Enterococcus faecalis</i> indocyanine green |
| title | The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating <i>Enterococcus faecalis</i> Biofilm from Infected Root Canals: An In Vitro Study |
| title_full | The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating <i>Enterococcus faecalis</i> Biofilm from Infected Root Canals: An In Vitro Study |
| title_fullStr | The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating <i>Enterococcus faecalis</i> Biofilm from Infected Root Canals: An In Vitro Study |
| title_full_unstemmed | The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating <i>Enterococcus faecalis</i> Biofilm from Infected Root Canals: An In Vitro Study |
| title_short | The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating <i>Enterococcus faecalis</i> Biofilm from Infected Root Canals: An In Vitro Study |
| title_sort | evaluation of sweeps plus antimicrobial photodynamic therapy with indocyanine green in eliminating i enterococcus faecalis i biofilm from infected root canals an in vitro study |
| topic | antimicrobial photodynamic therapy biofilms disinfection Er:YAG laser <i>Enterococcus faecalis</i> indocyanine green |
| url | https://www.mdpi.com/2227-9059/11/7/1850 |
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