Enhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCR
Abstract Background Diagnosis of infected individuals with Mycoplasma genitalium (MG) is often performed by real-time PCR or transcription-mediated amplification (TMA). A limitation of the MG-TMA assay is the relatively short time span of 24 h in which the collected urine is required to be transferr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-023-08499-z |
| _version_ | 1797453750746480640 |
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| author | Nikki Adriaens Anne-Marije Pennekamp Alje P. van Dam Sylvia M. Bruisten |
| author_facet | Nikki Adriaens Anne-Marije Pennekamp Alje P. van Dam Sylvia M. Bruisten |
| author_sort | Nikki Adriaens |
| collection | DOAJ |
| description | Abstract Background Diagnosis of infected individuals with Mycoplasma genitalium (MG) is often performed by real-time PCR or transcription-mediated amplification (TMA). A limitation of the MG-TMA assay is the relatively short time span of 24 h in which the collected urine is required to be transferred into a Urine Specimen Transport Tube, according to the manufacturer’s guidelines. If not transferred within 24 h, the manufacturer’s claimed sensitivity cannot be guaranteed anymore, and samples may instead be tested with an in-house validated real-time PCR, despite its recognized lower sensitivity. This study aimed to validate an exception to the sample transport and storage conditions of the MG-TMA assay as set by the manufacturer, being the prolongation of the acceptable testing time limit of 24 h. Methods From June to December 2022, first-void urines were collected from clients attending the clinic for sexual health in Amsterdam, the Netherlands. Urine samples that tested positive for MG by TMA assay at the day of collection were concomitantly stored at room (18–24 °C) and refrigerator temperature (4–8 °C) for 15 days. The stored urine samples were tested with both an in-house validated real-time PCR and MG-TMA assay after transfer of the original urine samples to the respective test tubes at 3, 7, 12 and 15 days post collection. Results In total, 47 MG-positive urine samples were collected, stored and tested for MG by real-time PCR and TMA assays. After storage at room temperature, the MG-detection rate by TMA was significantly higher compared to real-time PCR, at days 0 (p ≤ 0.001), 7 (p ≤ 0.001) and 12 (p < 0.05). After storage at refrigerator temperature, the MG-detection rate determined by TMA assay was significantly enhanced in comparison with real-time PCR at days 3 (p < 0.01), 7 (p ≤ 0.001) and 15 (p < 0.01). Conclusions This validation study showed that the MG-TMA assay has a superior detection rate in urine compared to real-time PCR, up to 15 days post sample collection and irrespective of storage temperature. Accepting urines older than 24 h to be tested by TMA will improve clinical diagnosis of MG infections. |
| first_indexed | 2024-03-09T15:27:23Z |
| format | Article |
| id | doaj.art-9162683aa5f24ae1be8a71eb24635303 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-03-09T15:27:23Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-9162683aa5f24ae1be8a71eb246353032023-11-26T12:27:58ZengBMCBMC Infectious Diseases1471-23342023-09-012311510.1186/s12879-023-08499-zEnhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCRNikki Adriaens0Anne-Marije Pennekamp1Alje P. van Dam2Sylvia M. Bruisten3Department of Infectious Diseases, Public Health Service of AmsterdamDepartment of Infectious Diseases, Public Health Service of AmsterdamDepartment of Infectious Diseases, Public Health Service of AmsterdamDepartment of Infectious Diseases, Public Health Service of AmsterdamAbstract Background Diagnosis of infected individuals with Mycoplasma genitalium (MG) is often performed by real-time PCR or transcription-mediated amplification (TMA). A limitation of the MG-TMA assay is the relatively short time span of 24 h in which the collected urine is required to be transferred into a Urine Specimen Transport Tube, according to the manufacturer’s guidelines. If not transferred within 24 h, the manufacturer’s claimed sensitivity cannot be guaranteed anymore, and samples may instead be tested with an in-house validated real-time PCR, despite its recognized lower sensitivity. This study aimed to validate an exception to the sample transport and storage conditions of the MG-TMA assay as set by the manufacturer, being the prolongation of the acceptable testing time limit of 24 h. Methods From June to December 2022, first-void urines were collected from clients attending the clinic for sexual health in Amsterdam, the Netherlands. Urine samples that tested positive for MG by TMA assay at the day of collection were concomitantly stored at room (18–24 °C) and refrigerator temperature (4–8 °C) for 15 days. The stored urine samples were tested with both an in-house validated real-time PCR and MG-TMA assay after transfer of the original urine samples to the respective test tubes at 3, 7, 12 and 15 days post collection. Results In total, 47 MG-positive urine samples were collected, stored and tested for MG by real-time PCR and TMA assays. After storage at room temperature, the MG-detection rate by TMA was significantly higher compared to real-time PCR, at days 0 (p ≤ 0.001), 7 (p ≤ 0.001) and 12 (p < 0.05). After storage at refrigerator temperature, the MG-detection rate determined by TMA assay was significantly enhanced in comparison with real-time PCR at days 3 (p < 0.01), 7 (p ≤ 0.001) and 15 (p < 0.01). Conclusions This validation study showed that the MG-TMA assay has a superior detection rate in urine compared to real-time PCR, up to 15 days post sample collection and irrespective of storage temperature. Accepting urines older than 24 h to be tested by TMA will improve clinical diagnosis of MG infections.https://doi.org/10.1186/s12879-023-08499-zSexually transmitted infectionMycoplasma genitaliumMolecular diagnostic techniquesNucleic acid amplification testTranscription-mediated amplificationAntimicrobial resistance |
| spellingShingle | Nikki Adriaens Anne-Marije Pennekamp Alje P. van Dam Sylvia M. Bruisten Enhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCR BMC Infectious Diseases Sexually transmitted infection Mycoplasma genitalium Molecular diagnostic techniques Nucleic acid amplification test Transcription-mediated amplification Antimicrobial resistance |
| title | Enhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCR |
| title_full | Enhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCR |
| title_fullStr | Enhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCR |
| title_full_unstemmed | Enhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCR |
| title_short | Enhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCR |
| title_sort | enhanced detection rate of mycoplasma genitalium in urine overtime by transcription mediated amplification in comparison to real time pcr |
| topic | Sexually transmitted infection Mycoplasma genitalium Molecular diagnostic techniques Nucleic acid amplification test Transcription-mediated amplification Antimicrobial resistance |
| url | https://doi.org/10.1186/s12879-023-08499-z |
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