Enhancement of rat spermatozoal hyperactivation by progesterone
Progesterone (P) is a well-known enhancer of hyperactivation which is associated with the success of in vitro fertilization (IVF). In this study, we examined whether P-enhanced hyperactivation affected IVF success in rats. When rat spermatozoa were exposed to 10, 20, and 40 ng/ml P, 20 ng/ml P enhan...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2023-09-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/69/5/69_2023-040/_pdf/-char/en |
| _version_ | 1797643138026700800 |
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| author | Yuki MIYAZAWA Masakatsu FUJINOKI |
| author_facet | Yuki MIYAZAWA Masakatsu FUJINOKI |
| author_sort | Yuki MIYAZAWA |
| collection | DOAJ |
| description | Progesterone (P) is a well-known enhancer of hyperactivation which is associated with the success of in vitro fertilization (IVF). In this study, we examined whether P-enhanced hyperactivation affected IVF success in rats. When rat spermatozoa were exposed to 10, 20, and 40 ng/ml P, 20 ng/ml P enhanced hyperactivation via the membrane progesterone receptor. In addition, the enhancement of hyperactivation by 20 ng/ml P was regulated by phospholipase C, transmembrane adenylate cyclase, and protein kinase A. However, 20 ng/ml P did not affect IVF success. These results suggest that 20 ng/ml P enhances rat spermatozoal hyperactivation through non-genomic pathways. Because the concentration of P changes during the estrous cycle, it seems that rat spermatozoa are hyperactivated in response to the oviductal environment. However, the effect of 20 ng/ml P does not seem to fully capacitate spermatozoa. |
| first_indexed | 2024-03-11T14:11:22Z |
| format | Article |
| id | doaj.art-9164a28607f14dea88f74d3e58626fcc |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T14:11:22Z |
| publishDate | 2023-09-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-9164a28607f14dea88f74d3e58626fcc2023-11-02T00:05:34ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002023-09-0169527929010.1262/jrd.2023-040jrdEnhancement of rat spermatozoal hyperactivation by progesteroneYuki MIYAZAWA0Masakatsu FUJINOKI1Research Laboratory of Laboratory Animals, Research Center for Laboratory Animals, Comprehensive Research Facilities for Advanced Medical Science, School of Medicine, Dokkyo Medical University, Tochigi 321-0293, JapanResearch Laboratory of Laboratory Animals, Research Center for Laboratory Animals, Comprehensive Research Facilities for Advanced Medical Science, School of Medicine, Dokkyo Medical University, Tochigi 321-0293, JapanProgesterone (P) is a well-known enhancer of hyperactivation which is associated with the success of in vitro fertilization (IVF). In this study, we examined whether P-enhanced hyperactivation affected IVF success in rats. When rat spermatozoa were exposed to 10, 20, and 40 ng/ml P, 20 ng/ml P enhanced hyperactivation via the membrane progesterone receptor. In addition, the enhancement of hyperactivation by 20 ng/ml P was regulated by phospholipase C, transmembrane adenylate cyclase, and protein kinase A. However, 20 ng/ml P did not affect IVF success. These results suggest that 20 ng/ml P enhances rat spermatozoal hyperactivation through non-genomic pathways. Because the concentration of P changes during the estrous cycle, it seems that rat spermatozoa are hyperactivated in response to the oviductal environment. However, the effect of 20 ng/ml P does not seem to fully capacitate spermatozoa.https://www.jstage.jst.go.jp/article/jrd/69/5/69_2023-040/_pdf/-char/encapacitationhyperactivationin vitro fertilizationprogesteronespermatozoon |
| spellingShingle | Yuki MIYAZAWA Masakatsu FUJINOKI Enhancement of rat spermatozoal hyperactivation by progesterone The Journal of Reproduction and Development capacitation hyperactivation in vitro fertilization progesterone spermatozoon |
| title | Enhancement of rat spermatozoal hyperactivation by progesterone |
| title_full | Enhancement of rat spermatozoal hyperactivation by progesterone |
| title_fullStr | Enhancement of rat spermatozoal hyperactivation by progesterone |
| title_full_unstemmed | Enhancement of rat spermatozoal hyperactivation by progesterone |
| title_short | Enhancement of rat spermatozoal hyperactivation by progesterone |
| title_sort | enhancement of rat spermatozoal hyperactivation by progesterone |
| topic | capacitation hyperactivation in vitro fertilization progesterone spermatozoon |
| url | https://www.jstage.jst.go.jp/article/jrd/69/5/69_2023-040/_pdf/-char/en |
| work_keys_str_mv | AT yukimiyazawa enhancementofratspermatozoalhyperactivationbyprogesterone AT masakatsufujinoki enhancementofratspermatozoalhyperactivationbyprogesterone |