The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades
Multistep enzymatic reactions (cascades) can be achieved by confining enzymes in synthetic materials, but ways to simultaneously energize, control and observe the reactions in real time are lacking. Here, bidirectional interconversion between aspartate and pyruvate by a five enzyme cascade trapped i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-20403-w |
| _version_ | 1818851291794767872 |
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| author | Giorgio Morello Clare F. Megarity Fraser A. Armstrong |
| author_facet | Giorgio Morello Clare F. Megarity Fraser A. Armstrong |
| author_sort | Giorgio Morello |
| collection | DOAJ |
| description | Multistep enzymatic reactions (cascades) can be achieved by confining enzymes in synthetic materials, but ways to simultaneously energize, control and observe the reactions in real time are lacking. Here, bidirectional interconversion between aspartate and pyruvate by a five enzyme cascade trapped in electrode nanopores, addressable by laptop commands, is demonstrated. |
| first_indexed | 2024-12-19T07:02:42Z |
| format | Article |
| id | doaj.art-91664833a62049bcaba7a424fead66f7 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T07:02:42Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-91664833a62049bcaba7a424fead66f72022-12-21T20:31:23ZengNature PortfolioNature Communications2041-17232021-01-011211910.1038/s41467-020-20403-wThe power of electrified nanoconfinement for energising, controlling and observing long enzyme cascadesGiorgio Morello0Clare F. Megarity1Fraser A. Armstrong2Inorganic Chemistry Laboratory, Department of Chemistry, University of OxfordInorganic Chemistry Laboratory, Department of Chemistry, University of OxfordInorganic Chemistry Laboratory, Department of Chemistry, University of OxfordMultistep enzymatic reactions (cascades) can be achieved by confining enzymes in synthetic materials, but ways to simultaneously energize, control and observe the reactions in real time are lacking. Here, bidirectional interconversion between aspartate and pyruvate by a five enzyme cascade trapped in electrode nanopores, addressable by laptop commands, is demonstrated.https://doi.org/10.1038/s41467-020-20403-w |
| spellingShingle | Giorgio Morello Clare F. Megarity Fraser A. Armstrong The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades Nature Communications |
| title | The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades |
| title_full | The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades |
| title_fullStr | The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades |
| title_full_unstemmed | The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades |
| title_short | The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades |
| title_sort | power of electrified nanoconfinement for energising controlling and observing long enzyme cascades |
| url | https://doi.org/10.1038/s41467-020-20403-w |
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