Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis
Background: Visceral leishmaniasis (VL) is a lethal parasitic disease, transmitted by sand fly vectors. Immunomodulatory properties of sand fly saliva proteins and their protective effects against Leishmania infection in pre-exposed animals suggest that a combination of an antigenic salivary protei...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2022-05-01
|
| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3466 |
| _version_ | 1818205170126815232 |
|---|---|
| author | Shima Fayaz Fariborz Bahrami Pezhman Fard-Esfahani Parviz Parvizi Golnaz Bahramali Soheila Ajdary |
| author_facet | Shima Fayaz Fariborz Bahrami Pezhman Fard-Esfahani Parviz Parvizi Golnaz Bahramali Soheila Ajdary |
| author_sort | Shima Fayaz |
| collection | DOAJ |
| description |
Background: Visceral leishmaniasis (VL) is a lethal parasitic disease, transmitted by sand fly vectors. Immunomodulatory properties of sand fly saliva proteins and their protective effects against Leishmania infection in pre-exposed animals suggest that a combination of an antigenic salivary protein along with a Leishmania antigen can be considered for designing a vaccine against leishmaniasis
Methods: Three different fusion forms of L. infantum hypothetical protein (LiHyV) in combination with Phlebotomus kandelakii salivary apyrase (PkanAp) were subjected to in-silico analyses. Major Histocompatibility Complex (MHC) class I and II epitopes in both humans and BALB/c mice were predicted. Antigenicity, immunogenicity, epitope conservancy, toxicity, and population coverage were also evaluated.
Results: Highly antigenic promiscuous epitopes consisting of truncated LiHyV (10-285) and full-length PkanAp (21-329) were identified in human and was named Model 1. This model contained 25 MHC-I and 141 MHC-II antigenic peptides which among them, MPANSDIRI and AQSLFDFSGLALDSN were fully conserved. LALDSNATV, RCSSALVSI, ALVSINVPL, SAVESGALF of MHC-I epitopes, and 28 MHC-II binding epitopes showed 60% conservancy among various clades. A population coverage with a rate of >75% in the Iranian population and >70% in the whole world was also identified.
Conclusion: Based on this in-silico approach, the predicted Model 1 could potentially be used as a vaccine candidate against VL.
|
| first_indexed | 2024-12-12T03:52:52Z |
| format | Article |
| id | doaj.art-9167321528e0463bb6fa0dacc6f5b8dc |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-12T03:52:52Z |
| publishDate | 2022-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-9167321528e0463bb6fa0dacc6f5b8dc2022-12-22T00:39:19ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2022-05-0117210.18502/ijpa.v17i2.9530Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral LeishmaniasisShima Fayaz0Fariborz Bahrami1Pezhman Fard-Esfahani2Parviz Parvizi3Golnaz Bahramali4Soheila Ajdary51. Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran 2. Department of Immunology, Pasteur Institute of Iran, Tehran, IranDepartment of Immunology, Pasteur Institute of Iran, Tehran, IranDepartment of Biochemistry, Pasteur Institute of Iran, Tehran, IranDepartment of Parasitology, Pasteur Institute of Iran, Tehran, IranDepartment of AIDS and Bloodborne Diseases, Pasteur Institute of Iran, Tehran, IranDepartment of Immunology, Pasteur Institute of Iran, Tehran, Iran Background: Visceral leishmaniasis (VL) is a lethal parasitic disease, transmitted by sand fly vectors. Immunomodulatory properties of sand fly saliva proteins and their protective effects against Leishmania infection in pre-exposed animals suggest that a combination of an antigenic salivary protein along with a Leishmania antigen can be considered for designing a vaccine against leishmaniasis Methods: Three different fusion forms of L. infantum hypothetical protein (LiHyV) in combination with Phlebotomus kandelakii salivary apyrase (PkanAp) were subjected to in-silico analyses. Major Histocompatibility Complex (MHC) class I and II epitopes in both humans and BALB/c mice were predicted. Antigenicity, immunogenicity, epitope conservancy, toxicity, and population coverage were also evaluated. Results: Highly antigenic promiscuous epitopes consisting of truncated LiHyV (10-285) and full-length PkanAp (21-329) were identified in human and was named Model 1. This model contained 25 MHC-I and 141 MHC-II antigenic peptides which among them, MPANSDIRI and AQSLFDFSGLALDSN were fully conserved. LALDSNATV, RCSSALVSI, ALVSINVPL, SAVESGALF of MHC-I epitopes, and 28 MHC-II binding epitopes showed 60% conservancy among various clades. A population coverage with a rate of >75% in the Iranian population and >70% in the whole world was also identified. Conclusion: Based on this in-silico approach, the predicted Model 1 could potentially be used as a vaccine candidate against VL. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3466ImmunoinformaticsLeishmania infantumPhlebotomus kandelakiiApyraseVaccine |
| spellingShingle | Shima Fayaz Fariborz Bahrami Pezhman Fard-Esfahani Parviz Parvizi Golnaz Bahramali Soheila Ajdary Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis Iranian Journal of Parasitology Immunoinformatics Leishmania infantum Phlebotomus kandelakii Apyrase Vaccine |
| title | Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis |
| title_full | Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis |
| title_fullStr | Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis |
| title_full_unstemmed | Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis |
| title_short | Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis |
| title_sort | immunoinformatics evaluation of a fusion protein composed of leishmania infantum lihyv and phlebotomus kandelakii apyrase as a vaccine candidate against visceral leishmaniasis |
| topic | Immunoinformatics Leishmania infantum Phlebotomus kandelakii Apyrase Vaccine |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3466 |
| work_keys_str_mv | AT shimafayaz immunoinformaticsevaluationofafusionproteincomposedofleishmaniainfantumlihyvandphlebotomuskandelakiiapyraseasavaccinecandidateagainstvisceralleishmaniasis AT fariborzbahrami immunoinformaticsevaluationofafusionproteincomposedofleishmaniainfantumlihyvandphlebotomuskandelakiiapyraseasavaccinecandidateagainstvisceralleishmaniasis AT pezhmanfardesfahani immunoinformaticsevaluationofafusionproteincomposedofleishmaniainfantumlihyvandphlebotomuskandelakiiapyraseasavaccinecandidateagainstvisceralleishmaniasis AT parvizparvizi immunoinformaticsevaluationofafusionproteincomposedofleishmaniainfantumlihyvandphlebotomuskandelakiiapyraseasavaccinecandidateagainstvisceralleishmaniasis AT golnazbahramali immunoinformaticsevaluationofafusionproteincomposedofleishmaniainfantumlihyvandphlebotomuskandelakiiapyraseasavaccinecandidateagainstvisceralleishmaniasis AT soheilaajdary immunoinformaticsevaluationofafusionproteincomposedofleishmaniainfantumlihyvandphlebotomuskandelakiiapyraseasavaccinecandidateagainstvisceralleishmaniasis |