Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility
Abstract Agrichemicals such as organophosphorus pesticides’ metabolites (OPPMs) are more hazardous and pervasive than their parent pesticides. Parental germline exposure to such xenobiotics leads to an elevated susceptibility towards reproductive failures e.g. sub- or in-fertility. This study sought...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-35541-6 |
| _version_ | 1797806790379831296 |
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| author | Shivani Chhillar Vipul Batra Arumugam Kumaresan Rakesh Kumar Ankit Pal Tirtha Kumar Datta |
| author_facet | Shivani Chhillar Vipul Batra Arumugam Kumaresan Rakesh Kumar Ankit Pal Tirtha Kumar Datta |
| author_sort | Shivani Chhillar |
| collection | DOAJ |
| description | Abstract Agrichemicals such as organophosphorus pesticides’ metabolites (OPPMs) are more hazardous and pervasive than their parent pesticides. Parental germline exposure to such xenobiotics leads to an elevated susceptibility towards reproductive failures e.g. sub- or in-fertility. This study sought to examine the effects of low-dose, acute OPPM exposure on mammalian sperm function using buffalo as the model organism. The buffalo spermatozoa were briefly (2 h) exposed to metabolites of the three most prevalent organophosphorus pesticides (OPPs) viz. Omethoate (from Dimethoate), paraoxon-methyl (from methyl/ethyl parathion) and 3, 5, 6-trichloro-2-pyridinol (from chlorpyrifos). Exposure to OPPMs resulted in compromised structural and functional integrity (dose-dependent) of the buffalo spermatozoa typified by elevated membrane damage, increased lipid peroxidation, precocious capacitation and tyrosine phosphorylation, perturbed mitochondrial activity and function and (P < 0.05). This led to a decline in the in vitro fertilizing ability (P < 0.01) of the exposed spermatozoa, as indicated by reduced cleavage and blastocyst formation rates. Preliminary data indicate that acute exposure to OPPMs, akin to their parent pesticides, induces biomolecular and physiological changes in spermatozoa that compromise their health and function ultimately affecting their fertility. This is the first study demonstrating the in vitro spermatotoxic effects of multiple OPPMs on male gamete functional integrity. |
| first_indexed | 2024-03-13T06:12:42Z |
| format | Article |
| id | doaj.art-9169d5684d994c66875dc32d931f96c5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T06:12:42Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9169d5684d994c66875dc32d931f96c52023-06-11T11:11:01ZengNature PortfolioScientific Reports2045-23222023-06-0113111510.1038/s41598-023-35541-6Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertilityShivani Chhillar0Vipul Batra1Arumugam Kumaresan2Rakesh Kumar3Ankit Pal4Tirtha Kumar Datta5Animal Genomics Lab., Animal Biotechnology Centre, ICAR-NDRI, National Dairy Research InstituteAnimal Genomics Lab., Animal Biotechnology Centre, ICAR-NDRI, National Dairy Research InstituteTheriogenelogy Lab., SRS of National Dairy Research InstituteAnimal Genomics Lab., Animal Biotechnology Centre, ICAR-NDRI, National Dairy Research InstituteAnimal Genomics Lab., Animal Biotechnology Centre, ICAR-NDRI, National Dairy Research InstituteAnimal Genomics Lab., Animal Biotechnology Centre, ICAR-NDRI, National Dairy Research InstituteAbstract Agrichemicals such as organophosphorus pesticides’ metabolites (OPPMs) are more hazardous and pervasive than their parent pesticides. Parental germline exposure to such xenobiotics leads to an elevated susceptibility towards reproductive failures e.g. sub- or in-fertility. This study sought to examine the effects of low-dose, acute OPPM exposure on mammalian sperm function using buffalo as the model organism. The buffalo spermatozoa were briefly (2 h) exposed to metabolites of the three most prevalent organophosphorus pesticides (OPPs) viz. Omethoate (from Dimethoate), paraoxon-methyl (from methyl/ethyl parathion) and 3, 5, 6-trichloro-2-pyridinol (from chlorpyrifos). Exposure to OPPMs resulted in compromised structural and functional integrity (dose-dependent) of the buffalo spermatozoa typified by elevated membrane damage, increased lipid peroxidation, precocious capacitation and tyrosine phosphorylation, perturbed mitochondrial activity and function and (P < 0.05). This led to a decline in the in vitro fertilizing ability (P < 0.01) of the exposed spermatozoa, as indicated by reduced cleavage and blastocyst formation rates. Preliminary data indicate that acute exposure to OPPMs, akin to their parent pesticides, induces biomolecular and physiological changes in spermatozoa that compromise their health and function ultimately affecting their fertility. This is the first study demonstrating the in vitro spermatotoxic effects of multiple OPPMs on male gamete functional integrity.https://doi.org/10.1038/s41598-023-35541-6 |
| spellingShingle | Shivani Chhillar Vipul Batra Arumugam Kumaresan Rakesh Kumar Ankit Pal Tirtha Kumar Datta Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility Scientific Reports |
| title | Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility |
| title_full | Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility |
| title_fullStr | Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility |
| title_full_unstemmed | Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility |
| title_short | Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility |
| title_sort | acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility |
| url | https://doi.org/10.1038/s41598-023-35541-6 |
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