Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo Findings
Background and Aim: Periodontopathogens require iron constituents for their growth and metabolism in subgingival crevice. In this study, C.rectus was detected and quantified by using 16s rDNA based PCR in chronic periodontitis and compared with the levels of serum iron, total iron binding capac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6189/14165_CE[Ra1]_F(AK)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
| Summary: | Background and Aim: Periodontopathogens require iron
constituents for their growth and metabolism in subgingival
crevice. In this study, C.rectus was detected and quantified
by using 16s rDNA based PCR in chronic periodontitis and
compared with the levels of serum iron, total iron binding
capacity and transferrin in chronic periodontitis and healthy
sites.
Materials and Methods: One hundred twenty subjects divided
into chronic periodontitis and healthy controls. Deep subgingival
plaque was collected and genomic DNA was extracted from
each sample analysed for C.rectus using 16s rRNA based PCR
analysis. Blood samples were collected from both groups for
estimation of serum iron, serum total iron binding capacity and
serum transferrin levels. The quantified bacterial count was
compared with blood samples. C. rectus was detected in both
groups.
Results: There was significant increase in bacterial count in
chronic periodontitis (p<0.05). Serum iron level was significantly
raised in healthy group. TIBC and transferrin levels were
elevated in periodontitis. Although these differences were
non-significant. Regression analysis showed significant linear
relationship between C.rectus counts and decreasing iron
levels and consequently increasing serum transferrin and TIBC
(p<0.05).
Conclusion: The preliminary in vivo findings suggests C.rectus
requires iron as a significant source of nutrition for its survival
and growth form its hosts in deeper subgingival sites. |
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| ISSN: | 2249-782X 0973-709X |