Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo Findings
Background and Aim: Periodontopathogens require iron constituents for their growth and metabolism in subgingival crevice. In this study, C.rectus was detected and quantified by using 16s rDNA based PCR in chronic periodontitis and compared with the levels of serum iron, total iron binding capac...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6189/14165_CE[Ra1]_F(AK)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
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| author | Raghavendra Shrishail Medikeri Suresh Vasant Lele Pradnya Prabhakar Mali Pinal Mahendra Jain Dattatray Anant Darawade Manjushri Raghavendra Medikeri |
| author_facet | Raghavendra Shrishail Medikeri Suresh Vasant Lele Pradnya Prabhakar Mali Pinal Mahendra Jain Dattatray Anant Darawade Manjushri Raghavendra Medikeri |
| author_sort | Raghavendra Shrishail Medikeri |
| collection | DOAJ |
| description | Background and Aim: Periodontopathogens require iron
constituents for their growth and metabolism in subgingival
crevice. In this study, C.rectus was detected and quantified
by using 16s rDNA based PCR in chronic periodontitis and
compared with the levels of serum iron, total iron binding
capacity and transferrin in chronic periodontitis and healthy
sites.
Materials and Methods: One hundred twenty subjects divided
into chronic periodontitis and healthy controls. Deep subgingival
plaque was collected and genomic DNA was extracted from
each sample analysed for C.rectus using 16s rRNA based PCR
analysis. Blood samples were collected from both groups for
estimation of serum iron, serum total iron binding capacity and
serum transferrin levels. The quantified bacterial count was
compared with blood samples. C. rectus was detected in both
groups.
Results: There was significant increase in bacterial count in
chronic periodontitis (p<0.05). Serum iron level was significantly
raised in healthy group. TIBC and transferrin levels were
elevated in periodontitis. Although these differences were
non-significant. Regression analysis showed significant linear
relationship between C.rectus counts and decreasing iron
levels and consequently increasing serum transferrin and TIBC
(p<0.05).
Conclusion: The preliminary in vivo findings suggests C.rectus
requires iron as a significant source of nutrition for its survival
and growth form its hosts in deeper subgingival sites. |
| first_indexed | 2024-12-13T06:52:31Z |
| format | Article |
| id | doaj.art-916b2fe9282c406eae075a1b3374d2b9 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-13T06:52:31Z |
| publishDate | 2015-07-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-916b2fe9282c406eae075a1b3374d2b92022-12-21T23:56:07ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-07-0197ZC26ZC3010.7860/JCDR/2015/14165.6189Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo FindingsRaghavendra Shrishail Medikeri0Suresh Vasant Lele1Pradnya Prabhakar Mali2Pinal Mahendra Jain3Dattatray Anant Darawade4Manjushri Raghavendra Medikeri5Reader, Department of Periodontology, Sinhgad Dental College and Hospital, Off sinhgad Road, Pune, Maharashtra, India.Professor and Head, Department of Periodontology, Sinhgad Dental College and Hospital, Off sinhgad Road, Pune, Maharashtra, India.Research Student, Department of Periodontology, Sinhgad Dental College and Hospital, Off sinhgad Road, Pune, Maharashtra, India.Research Student, Department of Periodontology, Sinhgad Dental College and Hospital, Off sinhgad Road, Pune, Maharashtra, India.Professor, Department of Oral and Maxillofacial Surgery, Rangta College of Dental Sciences and Research, Bhilai, Chattisgarh, India.Reader, Department of Oral Medicine and Radiology, Sinhgad Dental College and Hospital, Off sinhgad Road, Pune, Maharashtra, India.Background and Aim: Periodontopathogens require iron constituents for their growth and metabolism in subgingival crevice. In this study, C.rectus was detected and quantified by using 16s rDNA based PCR in chronic periodontitis and compared with the levels of serum iron, total iron binding capacity and transferrin in chronic periodontitis and healthy sites. Materials and Methods: One hundred twenty subjects divided into chronic periodontitis and healthy controls. Deep subgingival plaque was collected and genomic DNA was extracted from each sample analysed for C.rectus using 16s rRNA based PCR analysis. Blood samples were collected from both groups for estimation of serum iron, serum total iron binding capacity and serum transferrin levels. The quantified bacterial count was compared with blood samples. C. rectus was detected in both groups. Results: There was significant increase in bacterial count in chronic periodontitis (p<0.05). Serum iron level was significantly raised in healthy group. TIBC and transferrin levels were elevated in periodontitis. Although these differences were non-significant. Regression analysis showed significant linear relationship between C.rectus counts and decreasing iron levels and consequently increasing serum transferrin and TIBC (p<0.05). Conclusion: The preliminary in vivo findings suggests C.rectus requires iron as a significant source of nutrition for its survival and growth form its hosts in deeper subgingival sites.https://jcdr.net/articles/PDF/6189/14165_CE[Ra1]_F(AK)_PF1(PAK)_PFA(AK)_PF2(PAG).pdfc.rectuschronic periodontitismicrobiology/polymerase chain reaction |
| spellingShingle | Raghavendra Shrishail Medikeri Suresh Vasant Lele Pradnya Prabhakar Mali Pinal Mahendra Jain Dattatray Anant Darawade Manjushri Raghavendra Medikeri Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo Findings Journal of Clinical and Diagnostic Research c.rectus chronic periodontitis microbiology/polymerase chain reaction |
| title | Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo Findings |
| title_full | Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo Findings |
| title_fullStr | Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo Findings |
| title_full_unstemmed | Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo Findings |
| title_short | Effect of Camphylobacter rectus on Serum Iron and Transferrin- Invivo Findings |
| title_sort | effect of camphylobacter rectus on serum iron and transferrin invivo findings |
| topic | c.rectus chronic periodontitis microbiology/polymerase chain reaction |
| url | https://jcdr.net/articles/PDF/6189/14165_CE[Ra1]_F(AK)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
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