Extraction and Elevation of Cell-Free DNA under Mastitis and Heat Stress in Dairy Cattle

Extraction and Elevation of Cell-Free DNA under Mastitis and Heat Stress in Dairy Cattle

In this study, four methods (phenol-chloroform protocol, sodium iodide kit, QIAamp DNA Blood Mini Kit, and TIANamp Micro DNA Kit) were used to extract cell-free DNA (cfDNA) from cattle blood, and the yield and purity of cfDNA varied in four different methods from 0.36 to 0.84 ng/mL for yield and 0.6...

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Bibliographic Details
Main Authors: Yumei Chen, Zaheer Abbas, Lirong Hu, Ling Kang, Xiao Tan, Qing Xu, Yachun Wang
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Animals
Subjects:
cell-free DNA
extraction method
mastitis
heat stress
Holstein dairy cows
Online Access:https://www.mdpi.com/2076-2615/13/9/1487

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https://www.mdpi.com/2076-2615/13/9/1487

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