Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering
Regenerative medicine is increasingly focused on the development of biomaterials that facilitate cell adhesion and proliferation through the use of natural polymers, which have better biocompatibility and biodegradability. In this way, the use of hydrogels has been considered as a potential option f...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/12/5/1146 |
| _version_ | 1797567745107165184 |
|---|---|
| author | Victor Perez-Puyana Mercedes Jiménez-Rosado Alberto Romero Antonio Guerrero |
| author_facet | Victor Perez-Puyana Mercedes Jiménez-Rosado Alberto Romero Antonio Guerrero |
| author_sort | Victor Perez-Puyana |
| collection | DOAJ |
| description | Regenerative medicine is increasingly focused on the development of biomaterials that facilitate cell adhesion and proliferation through the use of natural polymers, which have better biocompatibility and biodegradability. In this way, the use of hydrogels has been considered as a potential option for tissue engineering due to their physical and chemical characteristics. However, few studies associate the raw materials properties and processing conditions with the final characteristics of hydrogels, which could condition their use as scaffolds for tissue engineering. In this context, the main objective of this work was the evaluation of type I collagen as raw material for the elaboration of hydrogels. In addition, gelation time, pH and temperature were evaluated as the most influential variables in the hydrogel processing method by rheological (time, strain and frequency sweep tests) and microstructural (Cryo-SEM) measurements. The results indicate that it is possible to obtain collagen hydrogels with adequate rheological and microstructural characteristics by selecting optimal processing conditions. However, further studies are necessary to assess their suitability for cell accommodation and growth. |
| first_indexed | 2024-03-10T19:46:50Z |
| format | Article |
| id | doaj.art-916bba04a7a54ebb92c7935a48798890 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T19:46:50Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-916bba04a7a54ebb92c7935a487988902023-11-20T00:45:29ZengMDPI AGPolymers2073-43602020-05-01125114610.3390/polym12051146Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue EngineeringVictor Perez-Puyana0Mercedes Jiménez-Rosado1Alberto Romero2Antonio Guerrero3Departamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Ingeniería Química, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Sevilla, SpainDepartamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Ingeniería Química, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Sevilla, SpainRegenerative medicine is increasingly focused on the development of biomaterials that facilitate cell adhesion and proliferation through the use of natural polymers, which have better biocompatibility and biodegradability. In this way, the use of hydrogels has been considered as a potential option for tissue engineering due to their physical and chemical characteristics. However, few studies associate the raw materials properties and processing conditions with the final characteristics of hydrogels, which could condition their use as scaffolds for tissue engineering. In this context, the main objective of this work was the evaluation of type I collagen as raw material for the elaboration of hydrogels. In addition, gelation time, pH and temperature were evaluated as the most influential variables in the hydrogel processing method by rheological (time, strain and frequency sweep tests) and microstructural (Cryo-SEM) measurements. The results indicate that it is possible to obtain collagen hydrogels with adequate rheological and microstructural characteristics by selecting optimal processing conditions. However, further studies are necessary to assess their suitability for cell accommodation and growth.https://www.mdpi.com/2073-4360/12/5/1146gelatinised collagenhydrogelrheologyCryo-SEMtissue engineering |
| spellingShingle | Victor Perez-Puyana Mercedes Jiménez-Rosado Alberto Romero Antonio Guerrero Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering Polymers gelatinised collagen hydrogel rheology Cryo-SEM tissue engineering |
| title | Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering |
| title_full | Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering |
| title_fullStr | Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering |
| title_full_unstemmed | Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering |
| title_short | Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering |
| title_sort | fabrication and characterization of hydrogels based on gelatinised collagen with potential application in tissue engineering |
| topic | gelatinised collagen hydrogel rheology Cryo-SEM tissue engineering |
| url | https://www.mdpi.com/2073-4360/12/5/1146 |
| work_keys_str_mv | AT victorperezpuyana fabricationandcharacterizationofhydrogelsbasedongelatinisedcollagenwithpotentialapplicationintissueengineering AT mercedesjimenezrosado fabricationandcharacterizationofhydrogelsbasedongelatinisedcollagenwithpotentialapplicationintissueengineering AT albertoromero fabricationandcharacterizationofhydrogelsbasedongelatinisedcollagenwithpotentialapplicationintissueengineering AT antonioguerrero fabricationandcharacterizationofhydrogelsbasedongelatinisedcollagenwithpotentialapplicationintissueengineering |