Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides
Bacterial-derived cellulose (BC) has been studied as a promising material for biomedical applications, including wound care, due to its biocompatibility, water-holding capacity, liquid/gas permeability, and handleability properties. Although BC has been studied as a dressing material for cutaneous w...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/3/1462 |
| _version_ | 1797318747114962944 |
|---|---|
| author | Elizabeth M. van Zyl Jeannine M. Coburn |
| author_facet | Elizabeth M. van Zyl Jeannine M. Coburn |
| author_sort | Elizabeth M. van Zyl |
| collection | DOAJ |
| description | Bacterial-derived cellulose (BC) has been studied as a promising material for biomedical applications, including wound care, due to its biocompatibility, water-holding capacity, liquid/gas permeability, and handleability properties. Although BC has been studied as a dressing material for cutaneous wounds, to date, BC inherently lacks antibacterial properties. The current research utilizes bifunctional chimeric peptides containing carbohydrate binding peptides (CBP; either a short version or a long version) and an antimicrobial peptide (AMP), KR-12. The secondary structure of the chimeric peptides was evaluated and confirmed that the α-helix structure of KR-12 was retained for both chimeric peptides evaluated (Long-CBP-KR12 and Short-CBP-KR12). Chimeric peptides and their individual components were assessed for cytotoxicity, where only higher concentrations of Short-CBP and longer timepoints of Short-CBP-KR12 exposure exhibited negative effects on metabolic activity, which was attributed to solubility issues. All KR-12-containing peptides exhibited antibacterial activity in solution against <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>). The lipopolysaccharide (LPS) binding capability of the peptides was evaluated and the Short-CBP-KR12 peptide exhibited enhanced LPS-binding capabilities compared to KR-12 alone. Both chimeric peptides were able to bind to BC and were observed to be retained on the surface over a 7-day period. All functionalized materials exhibited no adverse effects on the metabolic activity of both normal human dermal fibroblasts (NHDFs) and human epidermal keratinocyte (HaCaT) epithelial cells. Additionally, the BC tethered chimeric peptides exhibited antibacterial activity against <i>E. coli</i>. Overall, this research outlines the design and evaluation of chimeric CBP-KR12 peptides for developing antimicrobial BC membranes with potential applications in wound care. |
| first_indexed | 2024-03-08T03:56:50Z |
| format | Article |
| id | doaj.art-916bc42ba1b54763ab1ff3b63b2aeccf |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:56:50Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-916bc42ba1b54763ab1ff3b63b2aeccf2024-02-09T15:13:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01253146210.3390/ijms25031462Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding PeptidesElizabeth M. van Zyl0Jeannine M. Coburn1Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USADepartment of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USABacterial-derived cellulose (BC) has been studied as a promising material for biomedical applications, including wound care, due to its biocompatibility, water-holding capacity, liquid/gas permeability, and handleability properties. Although BC has been studied as a dressing material for cutaneous wounds, to date, BC inherently lacks antibacterial properties. The current research utilizes bifunctional chimeric peptides containing carbohydrate binding peptides (CBP; either a short version or a long version) and an antimicrobial peptide (AMP), KR-12. The secondary structure of the chimeric peptides was evaluated and confirmed that the α-helix structure of KR-12 was retained for both chimeric peptides evaluated (Long-CBP-KR12 and Short-CBP-KR12). Chimeric peptides and their individual components were assessed for cytotoxicity, where only higher concentrations of Short-CBP and longer timepoints of Short-CBP-KR12 exposure exhibited negative effects on metabolic activity, which was attributed to solubility issues. All KR-12-containing peptides exhibited antibacterial activity in solution against <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>). The lipopolysaccharide (LPS) binding capability of the peptides was evaluated and the Short-CBP-KR12 peptide exhibited enhanced LPS-binding capabilities compared to KR-12 alone. Both chimeric peptides were able to bind to BC and were observed to be retained on the surface over a 7-day period. All functionalized materials exhibited no adverse effects on the metabolic activity of both normal human dermal fibroblasts (NHDFs) and human epidermal keratinocyte (HaCaT) epithelial cells. Additionally, the BC tethered chimeric peptides exhibited antibacterial activity against <i>E. coli</i>. Overall, this research outlines the design and evaluation of chimeric CBP-KR12 peptides for developing antimicrobial BC membranes with potential applications in wound care.https://www.mdpi.com/1422-0067/25/3/1462KR-12antibacterial activitybacterial celluloseantimicrobial peptidescellulose binding peptideswound dressing |
| spellingShingle | Elizabeth M. van Zyl Jeannine M. Coburn Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides International Journal of Molecular Sciences KR-12 antibacterial activity bacterial cellulose antimicrobial peptides cellulose binding peptides wound dressing |
| title | Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides |
| title_full | Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides |
| title_fullStr | Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides |
| title_full_unstemmed | Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides |
| title_short | Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides |
| title_sort | functionalization of bacterial cellulose with the antimicrobial peptide kr 12 via chimerical cellulose binding peptides |
| topic | KR-12 antibacterial activity bacterial cellulose antimicrobial peptides cellulose binding peptides wound dressing |
| url | https://www.mdpi.com/1422-0067/25/3/1462 |
| work_keys_str_mv | AT elizabethmvanzyl functionalizationofbacterialcellulosewiththeantimicrobialpeptidekr12viachimericalcellulosebindingpeptides AT jeanninemcoburn functionalizationofbacterialcellulosewiththeantimicrobialpeptidekr12viachimericalcellulosebindingpeptides |