GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds.
MicroRNAs (miRNAs) are small regulators of gene expression that act on many different molecular and biochemical processes in eukaryotes. To date, miRNAs have not been considered in the current evaluation system for GM crops. In this study, small RNAs from the dry seeds of a GM wheat line overexpress...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5411081?pdf=render |
| _version_ | 1818298706998329344 |
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| author | Qiyan Jiang Xianjun Sun Fengjuan Niu Zheng Hu Rui Chen Hui Zhang |
| author_facet | Qiyan Jiang Xianjun Sun Fengjuan Niu Zheng Hu Rui Chen Hui Zhang |
| author_sort | Qiyan Jiang |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small regulators of gene expression that act on many different molecular and biochemical processes in eukaryotes. To date, miRNAs have not been considered in the current evaluation system for GM crops. In this study, small RNAs from the dry seeds of a GM wheat line overexpressing GmDREB1 and non-GM wheat cultivars were investigated using deep sequencing technology and bioinformatic approaches. As a result, 23 differentially expressed miRNAs in dry seeds were identified and confirmed between GM wheat and a non-GM acceptor. Notably, more differentially expressed tae-miRNAs between non-GM wheat varieties were found, indicating that the degree of variance between non-GM cultivars was considerably higher than that induced by the transgenic event. Most of the target genes of these differentially expressed miRNAs between GM wheat and a non-GM acceptor were associated with abiotic stress, in accordance with the product concept of GM wheat in improving drought and salt tolerance. Our data provided useful information and insights into the evaluation of miRNA expression in edible GM crops. |
| first_indexed | 2024-12-13T04:39:36Z |
| format | Article |
| id | doaj.art-916e041647ef46be9b1e4c917d64880c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T04:39:36Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-916e041647ef46be9b1e4c917d64880c2022-12-21T23:59:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017592410.1371/journal.pone.0175924GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds.Qiyan JiangXianjun SunFengjuan NiuZheng HuRui ChenHui ZhangMicroRNAs (miRNAs) are small regulators of gene expression that act on many different molecular and biochemical processes in eukaryotes. To date, miRNAs have not been considered in the current evaluation system for GM crops. In this study, small RNAs from the dry seeds of a GM wheat line overexpressing GmDREB1 and non-GM wheat cultivars were investigated using deep sequencing technology and bioinformatic approaches. As a result, 23 differentially expressed miRNAs in dry seeds were identified and confirmed between GM wheat and a non-GM acceptor. Notably, more differentially expressed tae-miRNAs between non-GM wheat varieties were found, indicating that the degree of variance between non-GM cultivars was considerably higher than that induced by the transgenic event. Most of the target genes of these differentially expressed miRNAs between GM wheat and a non-GM acceptor were associated with abiotic stress, in accordance with the product concept of GM wheat in improving drought and salt tolerance. Our data provided useful information and insights into the evaluation of miRNA expression in edible GM crops.http://europepmc.org/articles/PMC5411081?pdf=render |
| spellingShingle | Qiyan Jiang Xianjun Sun Fengjuan Niu Zheng Hu Rui Chen Hui Zhang GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds. PLoS ONE |
| title | GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds. |
| title_full | GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds. |
| title_fullStr | GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds. |
| title_full_unstemmed | GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds. |
| title_short | GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds. |
| title_sort | gmdreb1 overexpression affects the expression of micrornas in gm wheat seeds |
| url | http://europepmc.org/articles/PMC5411081?pdf=render |
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