Determination of Fosfomycin Susceptibility in Escherichia coli Isolates by Various Methods
Introduction: Because of its broad-spectrum activity and its safety profile, fosfomycin is often used as a first-line antibiotic for treating uncomplicated urinary tract infections. It is also one of the treatment options in various infections such as central nervous system, bone and joints, lung, s...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2022-03-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2022-01/94-102%20Duygu%20Ocal.pdf |
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| author | Duygu ÖCAL Ayşe Hande TÜRK Elif Sude LALE Zişan BAYANSAR Zeynep MARGÜN Cansu NAMLI Zeynep RİFAİOĞLU Ömer Can YÜCEL Beyza DOĞANAY ERDOĞAN |
| author_facet | Duygu ÖCAL Ayşe Hande TÜRK Elif Sude LALE Zişan BAYANSAR Zeynep MARGÜN Cansu NAMLI Zeynep RİFAİOĞLU Ömer Can YÜCEL Beyza DOĞANAY ERDOĞAN |
| author_sort | Duygu ÖCAL |
| collection | DOAJ |
| description | Introduction: Because of its broad-spectrum activity and its safety profile, fosfomycin is often used as a first-line antibiotic for treating uncomplicated urinary tract infections. It is also one of the treatment options in various infections such as central nervous system, bone and joints, lung, soft tissue infections and sepsis. Escherichia coli is frequently encountered as a causative agent in such infections and is responsible for more than 80% of community-acquired urinary tract infections. There is a need to develop a fast and easily applicable test to evaluate the efficacy of fosfomycin. The aim of this study is to determine the susceptibility of fosfomycin in Escherichia coli isolates obtained from clinical isolates by various methods and to evaluate these methods in terms of ease of use, sensitivity, and specificity.
Materials and Methods: A total 175 E. coli isolates obtained from various cultures sent from clinics between November 2020 and April 2021 were included in the study. Agar dilution, disc diffusion method, in-tube macrodilution method with fosfomycin discs and rapid fosfomycin/E. coli NP test was used for determining fosfomycin susceptibility of isolates. Results: 169 (97%) of E. coli isolates were by agar dilution, 165 (94%) by disc diffusion test (DDT), 165 (94%) by macrodilution tube using fosfomycin discs, 167 (95%) fast fosfomycin/E. coli NP test was found to be susceptible to fosfomycin. With agar dilution, MIC50= 1µg/mL, MIC90= 32µg/mL were determined. Considering the agar dilution as a reference method; the sensitivity and specificity of the DDT, macrodilution tube method using fosfomycin discs, rapid fosfomycin/E. coli test were 100% and 98.8%, 100% and 97.6%, 100% and 97.6%, respectively. Conclusion: As fosfomycin use increases, the demand for susceptibility testing of this agent is expected to rise. Despite numerous reports of its activity, broader clinical use of fosfomycin is hindered by the difficulty in performing susceptibility testing. Therefore, it is imperative to implement the best method to deliver reliable results in a timely manner. Laboratories need rapid, easily applicable and evaluable antimicrobial susceptibility tests. In this sense, the gold-standard method of agar dilution is not a valid option for many clinical laboratories. Although DDT is a more applicable test routinely, rapid fosfomycin/E. coli NP test is promising both in terms of speed and ease of application. |
| first_indexed | 2024-04-10T11:51:44Z |
| format | Article |
| id | doaj.art-9174b1b32e4c415d9e432dfe5b5a285f |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1308-5115 |
| language | English |
| last_indexed | 2024-04-10T11:51:44Z |
| publishDate | 2022-03-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-9174b1b32e4c415d9e432dfe5b5a285f2023-02-15T16:17:05ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1308-51152022-03-012719410210.5578/flora.20229908Determination of Fosfomycin Susceptibility in Escherichia coli Isolates by Various MethodsDuygu ÖCAL0https://orcid.org/0000-0001-9929-267XAyşe Hande TÜRK1https://orcid.org/0000-0003-1196-6161Elif Sude LALE2https://orcid.org/0000-0002-4231-7650Zişan BAYANSAR3https://orcid.org/0000-0001-7790-4487Zeynep MARGÜN4https://orcid.org/0000-0003-1165-9401Cansu NAMLI5https://orcid.org/0000-0002-2108-0914Zeynep RİFAİOĞLU6https://orcid.org/0000-0001-9715-8418Ömer Can YÜCEL7https://orcid.org/0000-0003-3410-1611Beyza DOĞANAY ERDOĞAN8https://orcid.org/0000-0001-8845-2287Department of Clinic Microbiology, Ankara University Faculty of Medicine, Ankara, TurkeyDepartment of Clinic Microbiology, Ankara University Faculty of Medicine, Ankara, Turkey4th Semester Students, Ankara University Faculty of Medicine, Ankara, Turkey4th Semester Students, Ankara University Faculty of Medicine, Ankara, Turkey4th Semester Students, Ankara University Faculty of Medicine, Ankara, Turkey4th Semester Students, Ankara University Faculty of Medicine, Ankara, Turkey4th Semester Students, Ankara University Faculty of Medicine, Ankara, Turkey4th Semester Students, Ankara University Faculty of Medicine, Ankara, TurkeyDepartment of Biostatistics, Ankara University Faculty of Medicine, Ankara, TurkeyIntroduction: Because of its broad-spectrum activity and its safety profile, fosfomycin is often used as a first-line antibiotic for treating uncomplicated urinary tract infections. It is also one of the treatment options in various infections such as central nervous system, bone and joints, lung, soft tissue infections and sepsis. Escherichia coli is frequently encountered as a causative agent in such infections and is responsible for more than 80% of community-acquired urinary tract infections. There is a need to develop a fast and easily applicable test to evaluate the efficacy of fosfomycin. The aim of this study is to determine the susceptibility of fosfomycin in Escherichia coli isolates obtained from clinical isolates by various methods and to evaluate these methods in terms of ease of use, sensitivity, and specificity. Materials and Methods: A total 175 E. coli isolates obtained from various cultures sent from clinics between November 2020 and April 2021 were included in the study. Agar dilution, disc diffusion method, in-tube macrodilution method with fosfomycin discs and rapid fosfomycin/E. coli NP test was used for determining fosfomycin susceptibility of isolates. Results: 169 (97%) of E. coli isolates were by agar dilution, 165 (94%) by disc diffusion test (DDT), 165 (94%) by macrodilution tube using fosfomycin discs, 167 (95%) fast fosfomycin/E. coli NP test was found to be susceptible to fosfomycin. With agar dilution, MIC50= 1µg/mL, MIC90= 32µg/mL were determined. Considering the agar dilution as a reference method; the sensitivity and specificity of the DDT, macrodilution tube method using fosfomycin discs, rapid fosfomycin/E. coli test were 100% and 98.8%, 100% and 97.6%, 100% and 97.6%, respectively. Conclusion: As fosfomycin use increases, the demand for susceptibility testing of this agent is expected to rise. Despite numerous reports of its activity, broader clinical use of fosfomycin is hindered by the difficulty in performing susceptibility testing. Therefore, it is imperative to implement the best method to deliver reliable results in a timely manner. Laboratories need rapid, easily applicable and evaluable antimicrobial susceptibility tests. In this sense, the gold-standard method of agar dilution is not a valid option for many clinical laboratories. Although DDT is a more applicable test routinely, rapid fosfomycin/E. coli NP test is promising both in terms of speed and ease of application.http://floradergisi.org/managete/fu_folder/2022-01/94-102%20Duygu%20Ocal.pdffosfomycinescherichia colisusceptibility testingagar dilution |
| spellingShingle | Duygu ÖCAL Ayşe Hande TÜRK Elif Sude LALE Zişan BAYANSAR Zeynep MARGÜN Cansu NAMLI Zeynep RİFAİOĞLU Ömer Can YÜCEL Beyza DOĞANAY ERDOĞAN Determination of Fosfomycin Susceptibility in Escherichia coli Isolates by Various Methods Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi fosfomycin escherichia coli susceptibility testing agar dilution |
| title | Determination of Fosfomycin Susceptibility in Escherichia coli Isolates by Various Methods |
| title_full | Determination of Fosfomycin Susceptibility in Escherichia coli Isolates by Various Methods |
| title_fullStr | Determination of Fosfomycin Susceptibility in Escherichia coli Isolates by Various Methods |
| title_full_unstemmed | Determination of Fosfomycin Susceptibility in Escherichia coli Isolates by Various Methods |
| title_short | Determination of Fosfomycin Susceptibility in Escherichia coli Isolates by Various Methods |
| title_sort | determination of fosfomycin susceptibility in escherichia coli isolates by various methods |
| topic | fosfomycin escherichia coli susceptibility testing agar dilution |
| url | http://floradergisi.org/managete/fu_folder/2022-01/94-102%20Duygu%20Ocal.pdf |
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