Mutations of ousA Alter the Virulence of Erwinia chrysanthemi
A negative correlation was observed between the aggressiveness of several Erwinia chrysanthemi strains on potato tuber and their osmotic tolerance. The disruption of the ousA gene encoding the major osmoprotectant uptake system highly enhanced bacterial virulence on potato tubers. The ousA disruptio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2005-02-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0150 |
| _version_ | 1818757278230118400 |
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| author | Karine Gloux Thierry Touze Yves Pagot Bernard Jouan Carlos Blanco |
| author_facet | Karine Gloux Thierry Touze Yves Pagot Bernard Jouan Carlos Blanco |
| author_sort | Karine Gloux |
| collection | DOAJ |
| description | A negative correlation was observed between the aggressiveness of several Erwinia chrysanthemi strains on potato tuber and their osmotic tolerance. The disruption of the ousA gene encoding the major osmoprotectant uptake system highly enhanced bacterial virulence on potato tubers. The ousA disruption also increased the maceration efficiency on potato tubers under anaerobic conditions. In the absence of oxygen, pectate lyase (Pel) production was significantly higher in the tissue macerated with the ousA- strain than with the wild type. Oxygen content is significantly different between infected and healthy tissues; therefore, ousA may be a contributory factor in the infection progression within the host. In minimal medium, ousA disruption enhanced Pel production and pelE expression only under micro-aerobiosis conditions. The effect on Pel was reversed by reintroduction of the ousA gene. The osmoprotectectants glycine betaine, proline betaine, and pipecolic acid are known to be taken up via OusA and to have an inhibitory effect on Pel production. However, their effects on Pel activity were not (glycine betaine) or only weakly (proline and pipecolic acid) affected by ousA disruption. Furthermore, no correlation was observed between their effects on Pel activities and their osmoprotection efficacies. The results demonstrate a relationship between E. chrysanthemi pathogenicity factors and the activity of ousA under low oxygen status. The evidence indicates that ousA and osmoprotectant effects on Pel are not linked to osmoregulation and that complex regulations exist between Pel production, ousA, and osmoprotection via compounds liberated during the plant infection. |
| first_indexed | 2024-12-18T06:08:24Z |
| format | Article |
| id | doaj.art-917575a6a1874d67a13e6d9ded55e091 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:08:24Z |
| publishDate | 2005-02-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-917575a6a1874d67a13e6d9ded55e0912022-12-21T21:18:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062005-02-0118215015710.1094/MPMI-18-0150Mutations of ousA Alter the Virulence of Erwinia chrysanthemiKarine GlouxThierry TouzeYves PagotBernard JouanCarlos BlancoA negative correlation was observed between the aggressiveness of several Erwinia chrysanthemi strains on potato tuber and their osmotic tolerance. The disruption of the ousA gene encoding the major osmoprotectant uptake system highly enhanced bacterial virulence on potato tubers. The ousA disruption also increased the maceration efficiency on potato tubers under anaerobic conditions. In the absence of oxygen, pectate lyase (Pel) production was significantly higher in the tissue macerated with the ousA- strain than with the wild type. Oxygen content is significantly different between infected and healthy tissues; therefore, ousA may be a contributory factor in the infection progression within the host. In minimal medium, ousA disruption enhanced Pel production and pelE expression only under micro-aerobiosis conditions. The effect on Pel was reversed by reintroduction of the ousA gene. The osmoprotectectants glycine betaine, proline betaine, and pipecolic acid are known to be taken up via OusA and to have an inhibitory effect on Pel production. However, their effects on Pel activity were not (glycine betaine) or only weakly (proline and pipecolic acid) affected by ousA disruption. Furthermore, no correlation was observed between their effects on Pel activities and their osmoprotection efficacies. The results demonstrate a relationship between E. chrysanthemi pathogenicity factors and the activity of ousA under low oxygen status. The evidence indicates that ousA and osmoprotectant effects on Pel are not linked to osmoregulation and that complex regulations exist between Pel production, ousA, and osmoprotection via compounds liberated during the plant infection.https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0150 |
| spellingShingle | Karine Gloux Thierry Touze Yves Pagot Bernard Jouan Carlos Blanco Mutations of ousA Alter the Virulence of Erwinia chrysanthemi Molecular Plant-Microbe Interactions |
| title | Mutations of ousA Alter the Virulence of Erwinia chrysanthemi |
| title_full | Mutations of ousA Alter the Virulence of Erwinia chrysanthemi |
| title_fullStr | Mutations of ousA Alter the Virulence of Erwinia chrysanthemi |
| title_full_unstemmed | Mutations of ousA Alter the Virulence of Erwinia chrysanthemi |
| title_short | Mutations of ousA Alter the Virulence of Erwinia chrysanthemi |
| title_sort | mutations of ousa alter the virulence of erwinia chrysanthemi |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0150 |
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