Evaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in Vietnam
Abstract Following the rising concern on environmental issues caused by conventional fossil-based plastics and depleting crude oil resources, polyhydroxyalkanoates (PHAs) are of great interest by scientists and biodegradable polymer market due to their outstanding properties which include high biode...
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| Format: | Article |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-28220-z |
| _version_ | 1797864918906568704 |
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| author | Nguyen Thi Tam Thu Le Huy Hoang Pham Kien Cuong Nguyen Viet-Linh Tran Thi Huyen Nga Dang Dinh Kim Yoong Kit Leong Le Thi Nhi-Cong |
| author_facet | Nguyen Thi Tam Thu Le Huy Hoang Pham Kien Cuong Nguyen Viet-Linh Tran Thi Huyen Nga Dang Dinh Kim Yoong Kit Leong Le Thi Nhi-Cong |
| author_sort | Nguyen Thi Tam Thu |
| collection | DOAJ |
| description | Abstract Following the rising concern on environmental issues caused by conventional fossil-based plastics and depleting crude oil resources, polyhydroxyalkanoates (PHAs) are of great interest by scientists and biodegradable polymer market due to their outstanding properties which include high biodegradability in various conditions and processing flexibility. Many polyhydroxyalkanoate-synthesizing microorganisms, including normal and halophilic bacteria, as well as algae, have been investigated for their performance in polyhydroxyalkanoate production. However, to the best of our knowledge, there is still limited studies on PHAs-producing marine yeast. In the present study, a halophilic yeast strain isolated from Spratly Island in Vietnam were investigated for its potential in polyhydroxyalkanoate biosynthesis by growing the yeast in Zobell marine agar medium (ZMA) containing Nile red dye. The strain was identified by 26S rDNA analysis as Pichia kudriavzevii TSLS24 and registered at Genbank database under code OL757724. The amount of polyhydroxyalkanoates synthesized was quantified by measuring the intracellular materials (predicted as poly(3-hydroxybutyrate) -PHB) by gravimetric method and subsequently confirmed by Fourier transform infrared (FTIR) spectroscopic and nuclear magnetic resonance (NMR) spectroscopic analyses. Under optimal growth conditions of 35 °C and pH 7 with supplementation of glucose and yeast extract at 20 and 10 gL−1, the isolated strain achieved poly(3-hydroxybutyrate) content and concentration of 43.4% and 1.8 gL−1 after 7 days of cultivation. The poly(3-hydroxybutyrate) produced demonstrated excellent biodegradability with degradation rate of 28% after 28 days of incubation in sea water. |
| first_indexed | 2024-04-09T22:59:42Z |
| format | Article |
| id | doaj.art-917726cc19a3435d89121407cc026291 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:59:42Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-917726cc19a3435d89121407cc0262912023-03-22T11:04:06ZengNature PortfolioScientific Reports2045-23222023-02-0113111410.1038/s41598-023-28220-zEvaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in VietnamNguyen Thi Tam Thu0Le Huy Hoang1Pham Kien Cuong2Nguyen Viet-Linh3Tran Thi Huyen Nga4Dang Dinh Kim5Yoong Kit Leong6Le Thi Nhi-Cong7Institute of New Technology, Academy of Military Science and TechnologyInstitute of New Technology, Academy of Military Science and TechnologyInstitute of New Technology, Academy of Military Science and TechnologyInstitute of Biotechnology, Vietnam Academy of Science and TechnologyUniversity of Science, Vietnam National University-HanoiInstitute of Environmental Technology, Vietnam Academy of Science and TechnologyDepartment of Chemical and Materials Engineering, Tunghai UniversityInstitute of Biotechnology, Vietnam Academy of Science and TechnologyAbstract Following the rising concern on environmental issues caused by conventional fossil-based plastics and depleting crude oil resources, polyhydroxyalkanoates (PHAs) are of great interest by scientists and biodegradable polymer market due to their outstanding properties which include high biodegradability in various conditions and processing flexibility. Many polyhydroxyalkanoate-synthesizing microorganisms, including normal and halophilic bacteria, as well as algae, have been investigated for their performance in polyhydroxyalkanoate production. However, to the best of our knowledge, there is still limited studies on PHAs-producing marine yeast. In the present study, a halophilic yeast strain isolated from Spratly Island in Vietnam were investigated for its potential in polyhydroxyalkanoate biosynthesis by growing the yeast in Zobell marine agar medium (ZMA) containing Nile red dye. The strain was identified by 26S rDNA analysis as Pichia kudriavzevii TSLS24 and registered at Genbank database under code OL757724. The amount of polyhydroxyalkanoates synthesized was quantified by measuring the intracellular materials (predicted as poly(3-hydroxybutyrate) -PHB) by gravimetric method and subsequently confirmed by Fourier transform infrared (FTIR) spectroscopic and nuclear magnetic resonance (NMR) spectroscopic analyses. Under optimal growth conditions of 35 °C and pH 7 with supplementation of glucose and yeast extract at 20 and 10 gL−1, the isolated strain achieved poly(3-hydroxybutyrate) content and concentration of 43.4% and 1.8 gL−1 after 7 days of cultivation. The poly(3-hydroxybutyrate) produced demonstrated excellent biodegradability with degradation rate of 28% after 28 days of incubation in sea water.https://doi.org/10.1038/s41598-023-28220-z |
| spellingShingle | Nguyen Thi Tam Thu Le Huy Hoang Pham Kien Cuong Nguyen Viet-Linh Tran Thi Huyen Nga Dang Dinh Kim Yoong Kit Leong Le Thi Nhi-Cong Evaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in Vietnam Scientific Reports |
| title | Evaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in Vietnam |
| title_full | Evaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in Vietnam |
| title_fullStr | Evaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in Vietnam |
| title_full_unstemmed | Evaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in Vietnam |
| title_short | Evaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in Vietnam |
| title_sort | evaluation of polyhydroxyalkanoate pha synthesis by pichia sp tsls24 yeast isolated in vietnam |
| url | https://doi.org/10.1038/s41598-023-28220-z |
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