Adaptation of the maize seedling seminal roots to drought: Essential role of plasma membrane H+-ATPases activity
To understand how maize plants adapt to drought, this study examines the role of plasma membrane proton pumps in root growth. This study delves into the physiological mechanisms through which maize plants respond to drought conditions, with a particular emphasis on elucidating the crucial role playe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2023-12-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://www.journalssystem.com/asbp/Adaptation-of-the-maize-seedling-seminal-roots-to-drought-Essential-role-of-plasma,177274,0,2.html |
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| author | Ewa Młodzińska-Michta Ewa Swiezewska Marta Hoffman-Sommer Natalia Piłka Mariusz Radkiewicz Paweł Jarzembowski |
| author_facet | Ewa Młodzińska-Michta Ewa Swiezewska Marta Hoffman-Sommer Natalia Piłka Mariusz Radkiewicz Paweł Jarzembowski |
| author_sort | Ewa Młodzińska-Michta |
| collection | DOAJ |
| description | To understand how maize plants adapt to drought, this study examines the role of plasma membrane proton pumps in root growth. This study delves into the physiological mechanisms through which maize plants respond to drought conditions, with a particular emphasis on elucidating the crucial role played by plasma membrane proton pumps in facilitating adaptive changes in root growth. Our results underscore the indispensable nature of these pumps in orchestrating precise modulation of root growth patterns during drought stress, highlighting their profound significance in stress responses. Additionally, the study reveals that osmotic stress alters lipid profiles in the plasma membrane, potentially impacting its functioning and the activity of membrane proteins. To understand the role of plasma membrane (PM) H + -ATPases in the adaptative response to osmotic stress and in the regulation of root growth in maize, we studied the gene expression and enzyme activity of PM H + -ATPases, as well as the changes in plant biomass and total root growth, in the seedlings of two maize cultivars: the drought-tolerant Calo cultivar and the drought-sensitive Abelardo. The seedlings were exposed to simulated drought for 24 h (treatment with 20% PEG). The enzyme activity and gene expression of the MHA4 H + -ATPase increased in the Calo variety but declined in Abelardo plants treated with PEG. The growth of roots in Abelardo plants exposed to 24 h of PEG treatment was reduced to almost 50% of the control. Conversely, for the Calo cultivar, there was no remarkable morpho-physiological difference between the roots of stressed and non-stressed plants. Therefore, the activity of the PM H + -ATPase seems to be an important factor for proper root growth during the adaptation of maize to drought. In addition, osmotic stress also induced changes in the levels of saturated polyisoprenoid alcohols in the plasma membrane fraction of maize roots. The increased levels of this class of lipids might modulate the physico-chemical properties of the PM lipid bilayer and thus affect its functioning and modify the activity of membrane proteins, such as PM H + -ATPases. |
| first_indexed | 2024-04-25T00:54:02Z |
| format | Article |
| id | doaj.art-9179fc5a740d46bfb1af8827437398bc |
| institution | Directory Open Access Journal |
| issn | 0001-6977 2083-9480 |
| language | English |
| last_indexed | 2024-04-25T00:54:02Z |
| publishDate | 2023-12-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-9179fc5a740d46bfb1af8827437398bc2024-03-11T12:58:00ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae0001-69772083-94802023-12-0192111510.5586/asbp/177274177274Adaptation of the maize seedling seminal roots to drought: Essential role of plasma membrane H+-ATPases activityEwa Młodzińska-Michta0https://orcid.org/0000-0002-6923-3764Ewa Swiezewska1Marta Hoffman-Sommer2Natalia Piłka3Mariusz Radkiewicz4Paweł Jarzembowski5Department of Plant Molecular Physiology, Wroclaw University, Kanonia 6/8, 50-328 Wrocław, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences (PAN) Warsaw, Adolfa Pawińskiego 5A, 02-106 Warszawa, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences (PAN) Warsaw, Adolfa Pawińskiego 5A, 02-106 Warszawa, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences (PAN) Warsaw, Adolfa Pawińskiego 5A, 02-106 Warszawa, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences (PAN) Warsaw, Adolfa Pawińskiego 5A, 02-106 Warszawa, PolandDepartment of Plant Biology, Institute of Biology, Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Science, Chełmońskiego 38c, 51-630 Wrocław, PolandTo understand how maize plants adapt to drought, this study examines the role of plasma membrane proton pumps in root growth. This study delves into the physiological mechanisms through which maize plants respond to drought conditions, with a particular emphasis on elucidating the crucial role played by plasma membrane proton pumps in facilitating adaptive changes in root growth. Our results underscore the indispensable nature of these pumps in orchestrating precise modulation of root growth patterns during drought stress, highlighting their profound significance in stress responses. Additionally, the study reveals that osmotic stress alters lipid profiles in the plasma membrane, potentially impacting its functioning and the activity of membrane proteins. To understand the role of plasma membrane (PM) H + -ATPases in the adaptative response to osmotic stress and in the regulation of root growth in maize, we studied the gene expression and enzyme activity of PM H + -ATPases, as well as the changes in plant biomass and total root growth, in the seedlings of two maize cultivars: the drought-tolerant Calo cultivar and the drought-sensitive Abelardo. The seedlings were exposed to simulated drought for 24 h (treatment with 20% PEG). The enzyme activity and gene expression of the MHA4 H + -ATPase increased in the Calo variety but declined in Abelardo plants treated with PEG. The growth of roots in Abelardo plants exposed to 24 h of PEG treatment was reduced to almost 50% of the control. Conversely, for the Calo cultivar, there was no remarkable morpho-physiological difference between the roots of stressed and non-stressed plants. Therefore, the activity of the PM H + -ATPase seems to be an important factor for proper root growth during the adaptation of maize to drought. In addition, osmotic stress also induced changes in the levels of saturated polyisoprenoid alcohols in the plasma membrane fraction of maize roots. The increased levels of this class of lipids might modulate the physico-chemical properties of the PM lipid bilayer and thus affect its functioning and modify the activity of membrane proteins, such as PM H + -ATPases.https://www.journalssystem.com/asbp/Adaptation-of-the-maize-seedling-seminal-roots-to-drought-Essential-role-of-plasma,177274,0,2.htmlabiotic stresscropproton pumpsroot physiologywater scarcity |
| spellingShingle | Ewa Młodzińska-Michta Ewa Swiezewska Marta Hoffman-Sommer Natalia Piłka Mariusz Radkiewicz Paweł Jarzembowski Adaptation of the maize seedling seminal roots to drought: Essential role of plasma membrane H+-ATPases activity Acta Societatis Botanicorum Poloniae abiotic stress crop proton pumps root physiology water scarcity |
| title | Adaptation of the maize seedling seminal roots to drought: Essential role of plasma membrane H+-ATPases activity |
| title_full | Adaptation of the maize seedling seminal roots to drought: Essential role of plasma membrane H+-ATPases activity |
| title_fullStr | Adaptation of the maize seedling seminal roots to drought: Essential role of plasma membrane H+-ATPases activity |
| title_full_unstemmed | Adaptation of the maize seedling seminal roots to drought: Essential role of plasma membrane H+-ATPases activity |
| title_short | Adaptation of the maize seedling seminal roots to drought: Essential role of plasma membrane H+-ATPases activity |
| title_sort | adaptation of the maize seedling seminal roots to drought essential role of plasma membrane h atpases activity |
| topic | abiotic stress crop proton pumps root physiology water scarcity |
| url | https://www.journalssystem.com/asbp/Adaptation-of-the-maize-seedling-seminal-roots-to-drought-Essential-role-of-plasma,177274,0,2.html |
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