Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria
Abstract Background and Objective: Because of increased resistance to antibiotics, side effects of chemical drugs and importance of medicinal plants, we aimed to assess the antibacterial effects of methanolic extract of the Polygonumbistorta plant on the E. coli (ATCC 15224), Ps. aeruginosa (ATCC 25...
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Format: | Article |
Language: | English |
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Golestan University of Medical Sciences
2014-07-01
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Series: | Medical Laboratory Journal |
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Online Access: | http://mlj.goums.ac.ir/article-1-448-en.html |
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author | T Ghelich M Hashemi Karouei I Gholampor Azizi |
author_facet | T Ghelich M Hashemi Karouei I Gholampor Azizi |
author_sort | T Ghelich |
collection | DOAJ |
description | Abstract Background and Objective: Because of increased resistance to antibiotics, side effects of chemical drugs and importance of medicinal plants, we aimed to assess the antibacterial effects of methanolic extract of the Polygonumbistorta plant on the E. coli (ATCC 15224), Ps. aeruginosa (ATCC 25619), B. subtilis (ATCC 6633) and Stap. Aureus (ATCC 25923). Material and Methods: After preparing the extract, its antibacterial effect was assessed via gel diffusion method, using disk / well diffusion methods to determine MIC and MBC Results: MIC of methanolic extract was 78 µg/ml for E. coli, 63×103 µg/ml for Pseudomonas aeruginosa, 39 µg/ml for Bacillus subtilis and 31×102 µg/ml for Staphylococcus aureus Conclusion: In spite of resistance of gram-negative bacteria to chemical agents, polygonum bistorta methanolic extract could inhibit the growth of E.coli and P. aeruginosa. Key words: Antibacterial, Bistorta, Escherichia Coli, Pseudomonas Aeruginosa |
first_indexed | 2024-12-11T15:14:10Z |
format | Article |
id | doaj.art-917ec44440c841d0aa5e40d71b7b4562 |
institution | Directory Open Access Journal |
issn | 2538-4449 |
language | English |
last_indexed | 2024-12-11T15:14:10Z |
publishDate | 2014-07-01 |
publisher | Golestan University of Medical Sciences |
record_format | Article |
series | Medical Laboratory Journal |
spelling | doaj.art-917ec44440c841d0aa5e40d71b7b45622022-12-22T01:00:39ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492014-07-01824147Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some BacteriaT Ghelich0M Hashemi Karouei1I Gholampor Azizi2 MSc of Microbiology, Islamic Azad University, Tonekabon, Iran Assistant Professor of Mycology, Department of Microbiology, Islamic Azad University, Tonekabon, Iran Assistant Professor of Mycology, Department of Biology, Islamic Azad University, Tonekabon, Iran Abstract Background and Objective: Because of increased resistance to antibiotics, side effects of chemical drugs and importance of medicinal plants, we aimed to assess the antibacterial effects of methanolic extract of the Polygonumbistorta plant on the E. coli (ATCC 15224), Ps. aeruginosa (ATCC 25619), B. subtilis (ATCC 6633) and Stap. Aureus (ATCC 25923). Material and Methods: After preparing the extract, its antibacterial effect was assessed via gel diffusion method, using disk / well diffusion methods to determine MIC and MBC Results: MIC of methanolic extract was 78 µg/ml for E. coli, 63×103 µg/ml for Pseudomonas aeruginosa, 39 µg/ml for Bacillus subtilis and 31×102 µg/ml for Staphylococcus aureus Conclusion: In spite of resistance of gram-negative bacteria to chemical agents, polygonum bistorta methanolic extract could inhibit the growth of E.coli and P. aeruginosa. Key words: Antibacterial, Bistorta, Escherichia Coli, Pseudomonas Aeruginosahttp://mlj.goums.ac.ir/article-1-448-en.htmlantibacterialbistortaescherichia colipseudomonas aeruginosa |
spellingShingle | T Ghelich M Hashemi Karouei I Gholampor Azizi Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria Medical Laboratory Journal antibacterial bistorta escherichia coli pseudomonas aeruginosa |
title | Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria |
title_full | Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria |
title_fullStr | Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria |
title_full_unstemmed | Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria |
title_short | Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria |
title_sort | antibacterial effect of methanolic extraction of polygonum bistorta on some bacteria |
topic | antibacterial bistorta escherichia coli pseudomonas aeruginosa |
url | http://mlj.goums.ac.ir/article-1-448-en.html |
work_keys_str_mv | AT tghelich antibacterialeffectofmethanolicextractionofpolygonumbistortaonsomebacteria AT mhashemikarouei antibacterialeffectofmethanolicextractionofpolygonumbistortaonsomebacteria AT igholamporazizi antibacterialeffectofmethanolicextractionofpolygonumbistortaonsomebacteria |