Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria

Abstract Background and Objective: Because of increased resistance to antibiotics, side effects of chemical drugs and importance of medicinal plants, we aimed to assess the antibacterial effects of methanolic extract of the Polygonumbistorta plant on the E. coli (ATCC 15224), Ps. aeruginosa (ATCC 25...

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Main Authors: T Ghelich, M Hashemi Karouei, I Gholampor Azizi
Format: Article
Language:English
Published: Golestan University of Medical Sciences 2014-07-01
Series:Medical Laboratory Journal
Subjects:
Online Access:http://mlj.goums.ac.ir/article-1-448-en.html
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author T Ghelich
M Hashemi Karouei
I Gholampor Azizi
author_facet T Ghelich
M Hashemi Karouei
I Gholampor Azizi
author_sort T Ghelich
collection DOAJ
description Abstract Background and Objective: Because of increased resistance to antibiotics, side effects of chemical drugs and importance of medicinal plants, we aimed to assess the antibacterial effects of methanolic extract of the Polygonumbistorta plant on the E. coli (ATCC 15224), Ps. aeruginosa (ATCC 25619), B. subtilis (ATCC 6633) and Stap. Aureus (ATCC 25923). Material and Methods: After preparing the extract, its antibacterial effect was assessed via gel diffusion method, using disk / well diffusion methods to determine MIC and MBC Results: MIC of methanolic extract was 78 µg/ml for E. coli, 63×103 µg/ml for Pseudomonas aeruginosa, 39 µg/ml for Bacillus subtilis and 31×102 µg/ml for Staphylococcus aureus Conclusion: In spite of resistance of gram-negative bacteria to chemical agents, polygonum bistorta methanolic extract could inhibit the growth of E.coli and P. aeruginosa. Key words: Antibacterial, Bistorta, Escherichia Coli, Pseudomonas Aeruginosa
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spelling doaj.art-917ec44440c841d0aa5e40d71b7b45622022-12-22T01:00:39ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492014-07-01824147Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some BacteriaT Ghelich0M Hashemi Karouei1I Gholampor Azizi2 MSc of Microbiology, Islamic Azad University, Tonekabon, Iran Assistant Professor of Mycology, Department of Microbiology, Islamic Azad University, Tonekabon, Iran Assistant Professor of Mycology, Department of Biology, Islamic Azad University, Tonekabon, Iran Abstract Background and Objective: Because of increased resistance to antibiotics, side effects of chemical drugs and importance of medicinal plants, we aimed to assess the antibacterial effects of methanolic extract of the Polygonumbistorta plant on the E. coli (ATCC 15224), Ps. aeruginosa (ATCC 25619), B. subtilis (ATCC 6633) and Stap. Aureus (ATCC 25923). Material and Methods: After preparing the extract, its antibacterial effect was assessed via gel diffusion method, using disk / well diffusion methods to determine MIC and MBC Results: MIC of methanolic extract was 78 µg/ml for E. coli, 63×103 µg/ml for Pseudomonas aeruginosa, 39 µg/ml for Bacillus subtilis and 31×102 µg/ml for Staphylococcus aureus Conclusion: In spite of resistance of gram-negative bacteria to chemical agents, polygonum bistorta methanolic extract could inhibit the growth of E.coli and P. aeruginosa. Key words: Antibacterial, Bistorta, Escherichia Coli, Pseudomonas Aeruginosahttp://mlj.goums.ac.ir/article-1-448-en.htmlantibacterialbistortaescherichia colipseudomonas aeruginosa
spellingShingle T Ghelich
M Hashemi Karouei
I Gholampor Azizi
Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria
Medical Laboratory Journal
antibacterial
bistorta
escherichia coli
pseudomonas aeruginosa
title Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria
title_full Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria
title_fullStr Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria
title_full_unstemmed Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria
title_short Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria
title_sort antibacterial effect of methanolic extraction of polygonum bistorta on some bacteria
topic antibacterial
bistorta
escherichia coli
pseudomonas aeruginosa
url http://mlj.goums.ac.ir/article-1-448-en.html
work_keys_str_mv AT tghelich antibacterialeffectofmethanolicextractionofpolygonumbistortaonsomebacteria
AT mhashemikarouei antibacterialeffectofmethanolicextractionofpolygonumbistortaonsomebacteria
AT igholamporazizi antibacterialeffectofmethanolicextractionofpolygonumbistortaonsomebacteria