Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cells
Sodium selenite supplementation at a concentration 50 nM before X-ray irradiation was suggested to protect non-cancerous human esophageal CHEK-1 cells from irradiation-induced damage. This present study investigated those effects on cancerous human esophageal cell line. The human cancer esophageal c...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Pensoft Publishers
2022-10-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/86473/download/pdf/ |
| _version_ | 1811229309911695360 |
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| author | Irma Melyani Puspitasari Rizky Abdulah |
| author_facet | Irma Melyani Puspitasari Rizky Abdulah |
| author_sort | Irma Melyani Puspitasari |
| collection | DOAJ |
| description | Sodium selenite supplementation at a concentration 50 nM before X-ray irradiation was suggested to protect non-cancerous human esophageal CHEK-1 cells from irradiation-induced damage. This present study investigated those effects on cancerous human esophageal cell line. The human cancer esophageal cell line, TE-8, was cultured and supplemented for the cytotoxicity assay, the GPx-1 activity, the cell viability assay, clonogenic assay and western blot analysis. An apoptosis biomarker, Cleaved PARP, was used. The results show that cell survival post-irradiation of supplemented-cells had the same effect as the cells treated by irradiation only, tended to decrease the cell viability (p=0.27), and decrease the survival rate of cancerous cells (p=1.00). The cleaved PARP level was higher in supplemented-and irradiated- cells than cells with irradiation alone. These results suggest that 50 nM sodium selenite supplementation prior to irradiation does not reduce the effectiveness of irradiation treatment on cancerous cells. |
| first_indexed | 2024-04-12T10:12:27Z |
| format | Article |
| id | doaj.art-91854e9adf264da79894b2b63c74c48d |
| institution | Directory Open Access Journal |
| issn | 2603-557X |
| language | English |
| last_indexed | 2024-04-12T10:12:27Z |
| publishDate | 2022-10-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj.art-91854e9adf264da79894b2b63c74c48d2022-12-22T03:37:16ZengPensoft PublishersPharmacia2603-557X2022-10-0169492192610.3897/pharmacia.69.e8647386473Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cellsIrma Melyani Puspitasari0Rizky Abdulah1Universitas PadjadjaranUniversitas PadjadjaranSodium selenite supplementation at a concentration 50 nM before X-ray irradiation was suggested to protect non-cancerous human esophageal CHEK-1 cells from irradiation-induced damage. This present study investigated those effects on cancerous human esophageal cell line. The human cancer esophageal cell line, TE-8, was cultured and supplemented for the cytotoxicity assay, the GPx-1 activity, the cell viability assay, clonogenic assay and western blot analysis. An apoptosis biomarker, Cleaved PARP, was used. The results show that cell survival post-irradiation of supplemented-cells had the same effect as the cells treated by irradiation only, tended to decrease the cell viability (p=0.27), and decrease the survival rate of cancerous cells (p=1.00). The cleaved PARP level was higher in supplemented-and irradiated- cells than cells with irradiation alone. These results suggest that 50 nM sodium selenite supplementation prior to irradiation does not reduce the effectiveness of irradiation treatment on cancerous cells.https://pharmacia.pensoft.net/article/86473/download/pdf/ |
| spellingShingle | Irma Melyani Puspitasari Rizky Abdulah Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cells Pharmacia |
| title | Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cells |
| title_full | Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cells |
| title_fullStr | Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cells |
| title_full_unstemmed | Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cells |
| title_short | Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cells |
| title_sort | sodium selenite supplementation does not reduce the effectiveness of x ray irradiation treatment on human cancerous esophageal te 8 cells |
| url | https://pharmacia.pensoft.net/article/86473/download/pdf/ |
| work_keys_str_mv | AT irmamelyanipuspitasari sodiumselenitesupplementationdoesnotreducetheeffectivenessofxrayirradiationtreatmentonhumancancerousesophagealte8cells AT rizkyabdulah sodiumselenitesupplementationdoesnotreducetheeffectivenessofxrayirradiationtreatmentonhumancancerousesophagealte8cells |