Enrichment and Characterization of PCB-Degrading Bacteria as Potential Seed Cultures for Bioremediation of Contaminated Soil
The main objective of our study was to obtain seed cultures for enhancing the transformation of polychlorinated biphenyls (PCBs) in contaminated soil of the transformer station in Zadar, Croatia, damaged during warfare activities in 1991. For enrichment, six soil samples were collected from differen...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2007-01-01
|
| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/48151 |
| _version_ | 1797423230935367680 |
|---|---|
| author | Dubravka Hršak Sanja Fingler Ernest Vončina Helena Ćetković Ana Begonja Kolar Nikolina Udiković Kolić Ines Petrić |
| author_facet | Dubravka Hršak Sanja Fingler Ernest Vončina Helena Ćetković Ana Begonja Kolar Nikolina Udiković Kolić Ines Petrić |
| author_sort | Dubravka Hršak |
| collection | DOAJ |
| description | The main objective of our study was to obtain seed cultures for enhancing the transformation of polychlorinated biphenyls (PCBs) in contaminated soil of the transformer station in Zadar, Croatia, damaged during warfare activities in 1991. For enrichment, six soil samples were collected from different polluted areas and microcosm approach, stimulating the growth of biphenyl-degrading bacteria, was employed. Enrichment experiments resulted in the selection of two fast growing mixed cultures TSZ7 and AIR1, originating from the soil of the transformer station and the airport area, respectively. Both cultures showed significant PCB-degrading activity (56 to 60 % of PCB50 mixture was reduced after a two-week cultivation). Furthermore, the cultures displayed similar PCB-degrading competence and reduced di-to tetrachlorobiphenyls more effectively than penta- to hepta-chlorobiphenyls. Strain Z6, identified as Rhodococcus erythropolis, was found to be the only culture member showing PCB-transformation potential similar to that of the mixed culture TSZ7, from which it was isolated. Based on the metabolites identified in the assay with the single congener 2,4,4’-chlorobiphenyl, we proposed that the strain Z6 was able to use both the 2,3-and 3,4-dioxygenase pathways. Furthermore, the identified metabolites suggested that beside these pathways another unidentified pathway might also be active in strain Z6. Based on the obtained results, the culture TSZ7 and the strain Z6 were designated as potential seed cultures for bioremediation of the contaminated soil. |
| first_indexed | 2024-03-09T07:43:25Z |
| format | Article |
| id | doaj.art-91875c19ec8341ed9f6649945895dca9 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:43:25Z |
| publishDate | 2007-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-91875c19ec8341ed9f6649945895dca92023-12-03T04:16:12ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062007-01-014511120Enrichment and Characterization of PCB-Degrading Bacteria as Potential Seed Cultures for Bioremediation of Contaminated SoilDubravka Hršak0Sanja Fingler1Ernest Vončina2Helena Ćetković3Ana Begonja Kolar4Nikolina Udiković Kolić5Ines Petrić6Ruđer Bošković Institute, Division for Marine and Environmental Research, POB 180, HR-10002 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, POB 291, HR-10001 Zagreb, CroatiaPublic Health Institute Maribor, Environmental Protection Institute, Prvomajska 1, SI-2000 Maribor, SloveniaRuđer Bošković Institute, Division of Molecular Biology, POB 180, HR-10002 Zagreb, CroatiaRuđer Bošković Institute, Division for Marine and Environmental Research, POB 180, HR-10002 Zagreb, CroatiaRuđer Bošković Institute, Division for Marine and Environmental Research, POB 180, HR-10002 Zagreb, CroatiaRuđer Bošković Institute, Division for Marine and Environmental Research, POB 180, HR-10002 Zagreb, CroatiaThe main objective of our study was to obtain seed cultures for enhancing the transformation of polychlorinated biphenyls (PCBs) in contaminated soil of the transformer station in Zadar, Croatia, damaged during warfare activities in 1991. For enrichment, six soil samples were collected from different polluted areas and microcosm approach, stimulating the growth of biphenyl-degrading bacteria, was employed. Enrichment experiments resulted in the selection of two fast growing mixed cultures TSZ7 and AIR1, originating from the soil of the transformer station and the airport area, respectively. Both cultures showed significant PCB-degrading activity (56 to 60 % of PCB50 mixture was reduced after a two-week cultivation). Furthermore, the cultures displayed similar PCB-degrading competence and reduced di-to tetrachlorobiphenyls more effectively than penta- to hepta-chlorobiphenyls. Strain Z6, identified as Rhodococcus erythropolis, was found to be the only culture member showing PCB-transformation potential similar to that of the mixed culture TSZ7, from which it was isolated. Based on the metabolites identified in the assay with the single congener 2,4,4’-chlorobiphenyl, we proposed that the strain Z6 was able to use both the 2,3-and 3,4-dioxygenase pathways. Furthermore, the identified metabolites suggested that beside these pathways another unidentified pathway might also be active in strain Z6. Based on the obtained results, the culture TSZ7 and the strain Z6 were designated as potential seed cultures for bioremediation of the contaminated soil.http://hrcak.srce.hr/file/48151PCB biodegradationPCB degrading bacteria2,3-dioxygenase activity3,4-dioxygenase activityRhodococcus erythropolis |
| spellingShingle | Dubravka Hršak Sanja Fingler Ernest Vončina Helena Ćetković Ana Begonja Kolar Nikolina Udiković Kolić Ines Petrić Enrichment and Characterization of PCB-Degrading Bacteria as Potential Seed Cultures for Bioremediation of Contaminated Soil Food Technology and Biotechnology PCB biodegradation PCB degrading bacteria 2,3-dioxygenase activity 3,4-dioxygenase activity Rhodococcus erythropolis |
| title | Enrichment and Characterization of PCB-Degrading Bacteria as Potential Seed Cultures for Bioremediation of Contaminated Soil |
| title_full | Enrichment and Characterization of PCB-Degrading Bacteria as Potential Seed Cultures for Bioremediation of Contaminated Soil |
| title_fullStr | Enrichment and Characterization of PCB-Degrading Bacteria as Potential Seed Cultures for Bioremediation of Contaminated Soil |
| title_full_unstemmed | Enrichment and Characterization of PCB-Degrading Bacteria as Potential Seed Cultures for Bioremediation of Contaminated Soil |
| title_short | Enrichment and Characterization of PCB-Degrading Bacteria as Potential Seed Cultures for Bioremediation of Contaminated Soil |
| title_sort | enrichment and characterization of pcb degrading bacteria as potential seed cultures for bioremediation of contaminated soil |
| topic | PCB biodegradation PCB degrading bacteria 2,3-dioxygenase activity 3,4-dioxygenase activity Rhodococcus erythropolis |
| url | http://hrcak.srce.hr/file/48151 |
| work_keys_str_mv | AT dubravkahrsak enrichmentandcharacterizationofpcbdegradingbacteriaaspotentialseedculturesforbioremediationofcontaminatedsoil AT sanjafingler enrichmentandcharacterizationofpcbdegradingbacteriaaspotentialseedculturesforbioremediationofcontaminatedsoil AT ernestvoncina enrichmentandcharacterizationofpcbdegradingbacteriaaspotentialseedculturesforbioremediationofcontaminatedsoil AT helenacetkovic enrichmentandcharacterizationofpcbdegradingbacteriaaspotentialseedculturesforbioremediationofcontaminatedsoil AT anabegonjakolar enrichmentandcharacterizationofpcbdegradingbacteriaaspotentialseedculturesforbioremediationofcontaminatedsoil AT nikolinaudikovickolic enrichmentandcharacterizationofpcbdegradingbacteriaaspotentialseedculturesforbioremediationofcontaminatedsoil AT inespetric enrichmentandcharacterizationofpcbdegradingbacteriaaspotentialseedculturesforbioremediationofcontaminatedsoil |