Behind the Scenes of PluriZyme Designs
Protein engineering is the design and modification of protein structures to optimize their functions or create novel functionalities for applications in biotechnology, medicine or industry. It represents an essential scientific solution for many of the environmental and societal challenges ahead of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Eng |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-4117/5/1/6 |
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| author | Ana Robles-Martín Sergi Roda Rubén Muñoz-Tafalla Victor Guallar |
| author_facet | Ana Robles-Martín Sergi Roda Rubén Muñoz-Tafalla Victor Guallar |
| author_sort | Ana Robles-Martín |
| collection | DOAJ |
| description | Protein engineering is the design and modification of protein structures to optimize their functions or create novel functionalities for applications in biotechnology, medicine or industry. It represents an essential scientific solution for many of the environmental and societal challenges ahead of us, such as polymer degradation. Unlike traditional chemical methods, enzyme-mediated degradation is selective and environmentally friendly and requires milder conditions. Computational methods will play a critical role in developing such solutions by enabling more efficient bioprospecting of natural polymer-degrading enzymes. They provide structural information, generate mechanistic studies, and formulate new hypotheses, facilitating the modeling and modification of these biocatalysts through enzyme engineering. The recent development of pluriZymes constitutes an example, providing a rational mechanism to integrate different biochemical processes into one single enzyme. In this review, we summarize our recent efforts in this line and introduce our early work towards polymer degradation using a pluriZyme-like technology, including our latest development in PET nanoparticle degradation. Moreover, we provide a comprehensive recipe for developing one’s own pluriZyme so that different laboratories can experiment with them and establish new limits. With modest computational resources and with help from this review, your first pluriZyme is one step closer. |
| first_indexed | 2024-04-24T18:20:25Z |
| format | Article |
| id | doaj.art-918843479472405ba371b252b1f1705c |
| institution | Directory Open Access Journal |
| issn | 2673-4117 |
| language | English |
| last_indexed | 2024-04-24T18:20:25Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Eng |
| spelling | doaj.art-918843479472405ba371b252b1f1705c2024-03-27T13:35:57ZengMDPI AGEng2673-41172024-01-01519110310.3390/eng5010006Behind the Scenes of PluriZyme DesignsAna Robles-Martín0Sergi Roda1Rubén Muñoz-Tafalla2Victor Guallar3Barcelona Supercomputing Center (BSC), 08034 Barcelona, SpainBarcelona Supercomputing Center (BSC), 08034 Barcelona, SpainBarcelona Supercomputing Center (BSC), 08034 Barcelona, SpainBarcelona Supercomputing Center (BSC), 08034 Barcelona, SpainProtein engineering is the design and modification of protein structures to optimize their functions or create novel functionalities for applications in biotechnology, medicine or industry. It represents an essential scientific solution for many of the environmental and societal challenges ahead of us, such as polymer degradation. Unlike traditional chemical methods, enzyme-mediated degradation is selective and environmentally friendly and requires milder conditions. Computational methods will play a critical role in developing such solutions by enabling more efficient bioprospecting of natural polymer-degrading enzymes. They provide structural information, generate mechanistic studies, and formulate new hypotheses, facilitating the modeling and modification of these biocatalysts through enzyme engineering. The recent development of pluriZymes constitutes an example, providing a rational mechanism to integrate different biochemical processes into one single enzyme. In this review, we summarize our recent efforts in this line and introduce our early work towards polymer degradation using a pluriZyme-like technology, including our latest development in PET nanoparticle degradation. Moreover, we provide a comprehensive recipe for developing one’s own pluriZyme so that different laboratories can experiment with them and establish new limits. With modest computational resources and with help from this review, your first pluriZyme is one step closer.https://www.mdpi.com/2673-4117/5/1/6pluriZymerational designactive siteprotein engineeringcomputational chemistry |
| spellingShingle | Ana Robles-Martín Sergi Roda Rubén Muñoz-Tafalla Victor Guallar Behind the Scenes of PluriZyme Designs Eng pluriZyme rational design active site protein engineering computational chemistry |
| title | Behind the Scenes of PluriZyme Designs |
| title_full | Behind the Scenes of PluriZyme Designs |
| title_fullStr | Behind the Scenes of PluriZyme Designs |
| title_full_unstemmed | Behind the Scenes of PluriZyme Designs |
| title_short | Behind the Scenes of PluriZyme Designs |
| title_sort | behind the scenes of plurizyme designs |
| topic | pluriZyme rational design active site protein engineering computational chemistry |
| url | https://www.mdpi.com/2673-4117/5/1/6 |
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