Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories
Abstract Engineering of microbial cells to produce high value chemicals is rapidly advancing. Yeast, bacteria and microalgae are being used to produce high value chemicals by utilizing widely available carbon sources. However, current extraction processes of many high value products from these cells...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-00189-7 |
| _version_ | 1819143349671559168 |
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| author | Santosh Pandit Oliver Konzock Kirsten Leistner VRSS Mokkapati Alessandra Merlo Jie Sun Ivan Mijakovic |
| author_facet | Santosh Pandit Oliver Konzock Kirsten Leistner VRSS Mokkapati Alessandra Merlo Jie Sun Ivan Mijakovic |
| author_sort | Santosh Pandit |
| collection | DOAJ |
| description | Abstract Engineering of microbial cells to produce high value chemicals is rapidly advancing. Yeast, bacteria and microalgae are being used to produce high value chemicals by utilizing widely available carbon sources. However, current extraction processes of many high value products from these cells are time- and labor-consuming and require toxic chemicals. This makes the extraction processes detrimental to the environment and not economically feasible. Hence, there is a demand for the development of simple, effective, and environmentally friendly method for the extraction of high value chemicals from these cell factories. Herein, we hypothesized that atomically thin edges of graphene having ability to interact with hydrophobic materials, could be used to extract high value lipids from cell factories. To achieve this, array of axially oriented graphene was deposited on iron nanoparticles. These coated nanoparticles were used to facilitate the release of intracellular lipids from Yarrowia lipolytica cells. Our treatment process can be integrated with the growth procedure and achieved the release of 50% of total cellular lipids from Y. lipolytica cells. Based on this result, we propose that nanoparticles coated with axially oriented graphene could pave efficient, environmentally friendly, and cost-effective way to release intracellular lipids from yeast cell factories. |
| first_indexed | 2024-12-22T12:24:50Z |
| format | Article |
| id | doaj.art-918a15f9e5824627b76ee7fdf10e8008 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T12:24:50Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-918a15f9e5824627b76ee7fdf10e80082022-12-21T18:25:50ZengNature PortfolioScientific Reports2045-23222021-10-011111910.1038/s41598-021-00189-7Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factoriesSantosh Pandit0Oliver Konzock1Kirsten Leistner2VRSS Mokkapati3Alessandra Merlo4Jie Sun5Ivan Mijakovic6Department of Biology and Biological Engineering, Chalmers University of TechnologyDepartment of Biology and Biological Engineering, Chalmers University of TechnologyThe MathWorks ABDepartment of Biology and Biological Engineering, Chalmers University of TechnologyDepartment of Biology and Biological Engineering, Chalmers University of TechnologyDepartment of Microtechnology and Nanoscience, Chalmers University of TechnologyDepartment of Biology and Biological Engineering, Chalmers University of TechnologyAbstract Engineering of microbial cells to produce high value chemicals is rapidly advancing. Yeast, bacteria and microalgae are being used to produce high value chemicals by utilizing widely available carbon sources. However, current extraction processes of many high value products from these cells are time- and labor-consuming and require toxic chemicals. This makes the extraction processes detrimental to the environment and not economically feasible. Hence, there is a demand for the development of simple, effective, and environmentally friendly method for the extraction of high value chemicals from these cell factories. Herein, we hypothesized that atomically thin edges of graphene having ability to interact with hydrophobic materials, could be used to extract high value lipids from cell factories. To achieve this, array of axially oriented graphene was deposited on iron nanoparticles. These coated nanoparticles were used to facilitate the release of intracellular lipids from Yarrowia lipolytica cells. Our treatment process can be integrated with the growth procedure and achieved the release of 50% of total cellular lipids from Y. lipolytica cells. Based on this result, we propose that nanoparticles coated with axially oriented graphene could pave efficient, environmentally friendly, and cost-effective way to release intracellular lipids from yeast cell factories.https://doi.org/10.1038/s41598-021-00189-7 |
| spellingShingle | Santosh Pandit Oliver Konzock Kirsten Leistner VRSS Mokkapati Alessandra Merlo Jie Sun Ivan Mijakovic Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories Scientific Reports |
| title | Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories |
| title_full | Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories |
| title_fullStr | Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories |
| title_full_unstemmed | Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories |
| title_short | Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories |
| title_sort | graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories |
| url | https://doi.org/10.1038/s41598-021-00189-7 |
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