Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying
The retention of the enzyme activity of alcohol dehydrogenase (ADH) has been studied in various drying processes such as spray drying. The aim of this study is to encapsulate ADH in mannitol, either with or without additive in order to limit the thermal denaturation of the enzyme during the drying p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-03-01
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| Series: | Pharmaceutics |
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| Online Access: | http://www.mdpi.com/1999-4923/6/1/185 |
| _version_ | 1811300130649800704 |
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| author | Hirokazu Shiga Hiromi Joreau Tze Loon Neoh Takeshi Furuta Hidefumi Yoshii |
| author_facet | Hirokazu Shiga Hiromi Joreau Tze Loon Neoh Takeshi Furuta Hidefumi Yoshii |
| author_sort | Hirokazu Shiga |
| collection | DOAJ |
| description | The retention of the enzyme activity of alcohol dehydrogenase (ADH) has been studied in various drying processes such as spray drying. The aim of this study is to encapsulate ADH in mannitol, either with or without additive in order to limit the thermal denaturation of the enzyme during the drying process. The retention of ADH activity was investigated at different drying temperatures. When mannitol was used, the encapsulated ADH was found inactive in all the dried powders. This is presumably due to the quick crystallization of mannitol during spray drying that resulted in the impairment of enzyme protection ability in comparison to its amorphous form. Maltodextin (dextrose equivalent = 11) was used to reduce the crystallization of mannitol. The addition of maltodextrin increased ADH activity and drastically changed the powder X-ray diffractogram of the spray-dried powders. |
| first_indexed | 2024-04-13T06:46:45Z |
| format | Article |
| id | doaj.art-918bbab499124342b009a3ca79efa106 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-13T06:46:45Z |
| publishDate | 2014-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-918bbab499124342b009a3ca79efa1062022-12-22T02:57:33ZengMDPI AGPharmaceutics1999-49232014-03-016118519410.3390/pharmaceutics6010185pharmaceutics6010185Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray DryingHirokazu Shiga0Hiromi Joreau1Tze Loon Neoh2Takeshi Furuta3Hidefumi Yoshii4Department of Applied Biological Science, Kagawa University, 2393 Ikenobe, Miki, Kita, Kagawa 761-0795, JapanDepartment of Food Engineering, ENSBANA University of Burgundy, 1 Esplanade Erasme-21000 Dijon, FranceDepartment of Applied Biological Science, Kagawa University, 2393 Ikenobe, Miki, Kita, Kagawa 761-0795, JapanDepartment of Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama Minami, Tottori 680-8552, JapanDepartment of Applied Biological Science, Kagawa University, 2393 Ikenobe, Miki, Kita, Kagawa 761-0795, JapanThe retention of the enzyme activity of alcohol dehydrogenase (ADH) has been studied in various drying processes such as spray drying. The aim of this study is to encapsulate ADH in mannitol, either with or without additive in order to limit the thermal denaturation of the enzyme during the drying process. The retention of ADH activity was investigated at different drying temperatures. When mannitol was used, the encapsulated ADH was found inactive in all the dried powders. This is presumably due to the quick crystallization of mannitol during spray drying that resulted in the impairment of enzyme protection ability in comparison to its amorphous form. Maltodextin (dextrose equivalent = 11) was used to reduce the crystallization of mannitol. The addition of maltodextrin increased ADH activity and drastically changed the powder X-ray diffractogram of the spray-dried powders.http://www.mdpi.com/1999-4923/6/1/185alcohol dehydrogenaseencapsulationspray dryingmannitol |
| spellingShingle | Hirokazu Shiga Hiromi Joreau Tze Loon Neoh Takeshi Furuta Hidefumi Yoshii Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying Pharmaceutics alcohol dehydrogenase encapsulation spray drying mannitol |
| title | Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying |
| title_full | Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying |
| title_fullStr | Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying |
| title_full_unstemmed | Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying |
| title_short | Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying |
| title_sort | encapsulation of alcohol dehydrogenase in mannitol by spray drying |
| topic | alcohol dehydrogenase encapsulation spray drying mannitol |
| url | http://www.mdpi.com/1999-4923/6/1/185 |
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