Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum
Abstract Euryodendron excelsum H.T. Chang is a rare and endangered woody plant endemic to China. It is very important to conserve and propagate this species from extinction. In this study, leaves and petioles from the axillary shoots in vitro were used as explants to culture on the different plant g...
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| Format: | Article |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24744-y |
| _version_ | 1811315048765718528 |
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| author | Yuping Xiong Shuangyan Chen Teng Wu Kunlin Wu Yuan Li Xinhua Zhang Jaime A. Teixeira da Silva Songjun Zeng Guohua Ma |
| author_facet | Yuping Xiong Shuangyan Chen Teng Wu Kunlin Wu Yuan Li Xinhua Zhang Jaime A. Teixeira da Silva Songjun Zeng Guohua Ma |
| author_sort | Yuping Xiong |
| collection | DOAJ |
| description | Abstract Euryodendron excelsum H.T. Chang is a rare and endangered woody plant endemic to China. It is very important to conserve and propagate this species from extinction. In this study, leaves and petioles from the axillary shoots in vitro were used as explants to culture on the different plant growth regulator (PGR) woody plant medium (WPM) and establish an efficient shoot proliferation and plant regeneration system. WPM supplemented with 1.0 mg/L 2,4-D induced callus dedifferentiated into buds and somatic embryos on various media,including PGR-free WPM. However, only adventitious shoots formed on WPM with 1.0 mg/L of cytokinins such as 6-benzyladenine (BA), kinetin (KIN) or thidiazuron (TDZ). When another cytokinin, zeatin, was used, somatic embryos were induced directly from From cut surface of these explants. Adventitious roots could be induced from both explants on WPM with 1.0 mg/L α-naphthaleneacetic acid (NAA). Somatic embryos cultured in PGR-free WPM or WPM with 0.2 mg/L NAA developed roots. Plantlets derived from somatic embryos were transferred to a peat: sand (1:1, v/v) substrate, and showed survival rates of 64.3% at 30 days and 54.6% at 90 days. Callus clumps with adventitious shoot buds that were transferred to WPM containing 1.0 mg/L BA and 0.2 mg/L NAA generated a mean 3.3 multiple shoots. Callus-derived shoots regenerated and rooted successfully (100%) on agar-free vermiculite-based WPM with 0.5 μM NAA after 30 d. Plantlets transplanted to peat soil: vermiculite (1:1, v/v) displayed the highest survival (96.7%) after three months. |
| first_indexed | 2024-04-13T11:23:18Z |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T11:23:18Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-918d55da1a5d4c6da7bdece66a4515652022-12-22T02:48:45ZengNature PortfolioScientific Reports2045-23222022-11-011211910.1038/s41598-022-24744-yShoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsumYuping Xiong0Shuangyan Chen1Teng Wu2Kunlin Wu3Yuan Li4Xinhua Zhang5Jaime A. Teixeira da Silva6Songjun Zeng7Guohua Ma8Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, The Chinese Academy of SciencesInstitute of Nanfan and Seed Industry, Guangdong Academy of SciencesGuangzhou Minghui Landscape Technology Development Co, Ltd.Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, The Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, The Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, The Chinese Academy of SciencesIndependent ResearcherGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, The Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, The Chinese Academy of SciencesAbstract Euryodendron excelsum H.T. Chang is a rare and endangered woody plant endemic to China. It is very important to conserve and propagate this species from extinction. In this study, leaves and petioles from the axillary shoots in vitro were used as explants to culture on the different plant growth regulator (PGR) woody plant medium (WPM) and establish an efficient shoot proliferation and plant regeneration system. WPM supplemented with 1.0 mg/L 2,4-D induced callus dedifferentiated into buds and somatic embryos on various media,including PGR-free WPM. However, only adventitious shoots formed on WPM with 1.0 mg/L of cytokinins such as 6-benzyladenine (BA), kinetin (KIN) or thidiazuron (TDZ). When another cytokinin, zeatin, was used, somatic embryos were induced directly from From cut surface of these explants. Adventitious roots could be induced from both explants on WPM with 1.0 mg/L α-naphthaleneacetic acid (NAA). Somatic embryos cultured in PGR-free WPM or WPM with 0.2 mg/L NAA developed roots. Plantlets derived from somatic embryos were transferred to a peat: sand (1:1, v/v) substrate, and showed survival rates of 64.3% at 30 days and 54.6% at 90 days. Callus clumps with adventitious shoot buds that were transferred to WPM containing 1.0 mg/L BA and 0.2 mg/L NAA generated a mean 3.3 multiple shoots. Callus-derived shoots regenerated and rooted successfully (100%) on agar-free vermiculite-based WPM with 0.5 μM NAA after 30 d. Plantlets transplanted to peat soil: vermiculite (1:1, v/v) displayed the highest survival (96.7%) after three months.https://doi.org/10.1038/s41598-022-24744-y |
| spellingShingle | Yuping Xiong Shuangyan Chen Teng Wu Kunlin Wu Yuan Li Xinhua Zhang Jaime A. Teixeira da Silva Songjun Zeng Guohua Ma Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum Scientific Reports |
| title | Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum |
| title_full | Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum |
| title_fullStr | Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum |
| title_full_unstemmed | Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum |
| title_short | Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum |
| title_sort | shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered euryodendron excelsum |
| url | https://doi.org/10.1038/s41598-022-24744-y |
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