Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC
In this study, a reversed-phase high performance liquid-chromatography coupled to ultraviolet detection (RP-HPLC/UV) method for simultaneous determination of six major bioactive constituents (caffeic acid, chlorogenic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, 4,5-dicaffeoyl quini...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-03-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050099 |
| _version_ | 1811162819629940736 |
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| author | Zhifeng FU He ZHOU Yan SHI Jianwen LU |
| author_facet | Zhifeng FU He ZHOU Yan SHI Jianwen LU |
| author_sort | Zhifeng FU |
| collection | DOAJ |
| description | In this study, a reversed-phase high performance liquid-chromatography coupled to ultraviolet detection (RP-HPLC/UV) method for simultaneous determination of six major bioactive constituents (caffeic acid, chlorogenic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, 4,5-dicaffeoyl quinic acid and isoquercetin) in sweet potato leaves was developed and validated after optimization of various chromatographic conditions and other experimental parameters. The chromatographic separation was achieved on an Agilent ZORBAX SB-C18 reserved-phase column (4.6 mm×250 mm, 5 μm) using a gradient elution program with a mobile phase consisting of 0.1% formic acid aqueous solution (solvent A) and acetonitrile (solvent B) (0~12 min, 10%~25% B; 12~18 min, 25% B), at a flow rate of 0.8 mL/min, an operating temperature of 30 ℃, and a wavelength of 326 nm. The calibration curves were linear in the range of 0.25~25.0, 0.5~50.0, 5.0~100.0, 10.0~200.0, 5.0~100.0, 0.5~50.0 μg/mL for caffeic acid, chlorogenic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, 4,5-dicaffeoyl quinic acid and isoquercetin, respectively. The present method was fast (18 min), and demonstrated acceptable values for linearity (R2≥0.998), stability (RSD≤1.97%), recovery (95.14%~103.22%) and precision (RSD≤1.56%). Additionally, the new method was successfully applied to determine components in sweet potato leaves from ten different regions of Jiangxi Province, and the results indicated that the sample harvested in Fengcheng city of Yichun contained higher total phenol content than those collected in other regions. |
| first_indexed | 2024-04-10T06:35:16Z |
| format | Article |
| id | doaj.art-9191c5efa6a24f879ab7e3720015fd4e |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-10T06:35:16Z |
| publishDate | 2023-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-9191c5efa6a24f879ab7e3720015fd4e2023-03-01T01:21:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-03-0144529229810.13386/j.issn1002-0306.20220500992022050099-5Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLCZhifeng FU0He ZHOU1Yan SHI2Jianwen LU3Food and Drug Inspection Sub Center of Jiujiang Inspection, Testing and Certification Center, Jiujiang 332000, ChinaFood and Drug Inspection Sub Center of Jiujiang Inspection, Testing and Certification Center, Jiujiang 332000, ChinaStare Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaSelenium Resources Development and Utilization Center of Yichun, Yichun 336000, ChinaIn this study, a reversed-phase high performance liquid-chromatography coupled to ultraviolet detection (RP-HPLC/UV) method for simultaneous determination of six major bioactive constituents (caffeic acid, chlorogenic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, 4,5-dicaffeoyl quinic acid and isoquercetin) in sweet potato leaves was developed and validated after optimization of various chromatographic conditions and other experimental parameters. The chromatographic separation was achieved on an Agilent ZORBAX SB-C18 reserved-phase column (4.6 mm×250 mm, 5 μm) using a gradient elution program with a mobile phase consisting of 0.1% formic acid aqueous solution (solvent A) and acetonitrile (solvent B) (0~12 min, 10%~25% B; 12~18 min, 25% B), at a flow rate of 0.8 mL/min, an operating temperature of 30 ℃, and a wavelength of 326 nm. The calibration curves were linear in the range of 0.25~25.0, 0.5~50.0, 5.0~100.0, 10.0~200.0, 5.0~100.0, 0.5~50.0 μg/mL for caffeic acid, chlorogenic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, 4,5-dicaffeoyl quinic acid and isoquercetin, respectively. The present method was fast (18 min), and demonstrated acceptable values for linearity (R2≥0.998), stability (RSD≤1.97%), recovery (95.14%~103.22%) and precision (RSD≤1.56%). Additionally, the new method was successfully applied to determine components in sweet potato leaves from ten different regions of Jiangxi Province, and the results indicated that the sample harvested in Fengcheng city of Yichun contained higher total phenol content than those collected in other regions.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050099hplccaffeoylquinic acidsisoquercitrinsimultaneous determinationsweet potato leaves |
| spellingShingle | Zhifeng FU He ZHOU Yan SHI Jianwen LU Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC Shipin gongye ke-ji hplc caffeoylquinic acids isoquercitrin simultaneous determination sweet potato leaves |
| title | Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC |
| title_full | Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC |
| title_fullStr | Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC |
| title_full_unstemmed | Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC |
| title_short | Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC |
| title_sort | simultaneous determination of five phenolic acids and isoquercitrin in sweet potato ipomoea batatas l lam leaves by hplc |
| topic | hplc caffeoylquinic acids isoquercitrin simultaneous determination sweet potato leaves |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050099 |
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