Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up
OBJECTIVE: The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the clinical status of patients undergoing liver transplant. INTRODUCTION: The human 	...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier España
2011-01-01
|
| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000600005 |
| _version_ | 1811316899249651712 |
|---|---|
| author | Fernanda Aparecida Costa Marcelo Naoki Soki Paula Durante Andrade Sandra Helena Alves Bonon Ronaldo Luis Thomasini Ana Maria Sampaio Marcelo de Carvalho Ramos Claudio Lúcio Rossi Teresa Cristina Cavalcanti Ilka de Fatima Boin Marília Leonard Luiz Sérgio Leonard Raquel Bello Stucchi Sandra Cecília Botelho Costa |
| author_facet | Fernanda Aparecida Costa Marcelo Naoki Soki Paula Durante Andrade Sandra Helena Alves Bonon Ronaldo Luis Thomasini Ana Maria Sampaio Marcelo de Carvalho Ramos Claudio Lúcio Rossi Teresa Cristina Cavalcanti Ilka de Fatima Boin Marília Leonard Luiz Sérgio Leonard Raquel Bello Stucchi Sandra Cecília Botelho Costa |
| author_sort | Fernanda Aparecida Costa |
| collection | DOAJ |
| description | OBJECTIVE: The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the clinical status of patients undergoing liver transplant. INTRODUCTION: The human β-herpesviruses, including cytomegalovirus and human herpesvirus 6, are ubiquitous among human populations. Active infections of human herpesvirus 6 and cytomegalovirus are common after liver transplantation, possibly induced and facilitated by allograft rejection and immunosuppressive therapy. Both viruses affect the success of the transplant procedure. METHODS: Thirty patients submitted to liver transplant at the Liver Transplant Unit, at the Gastro Center, State University of Campinas, SP, Brazil, were studied prospectively from six months to one year, nested-polymerase chain reaction for cytomegalovirus and human herpesvirus 6 DNA detections. Two or more consecutive positive nested-polymerase chain reaction were considered indicative of active infection. RESULTS: Active infection by cytomegalovirus was detected in 13/30 (43.3%) patients, median time to first cytomegalovirus detection was 29 days after transplantation (range: 0-99 days). Active infection by human herpesvirus 6 was detected in 12/30 (40%) patients, median time to first human herpesvirus 6 detection was 23.5 days after transplantation (range: 0-273 days). The time-related appearance of each virus was not statistically different (p = 0.49). Rejection of the transplanted liver was observed in 16.7% (5/30) of the patients. The present analysis showed that human herpesvirus 6 and/or cytomegalovirus active infections were frequent in liver transplant recipients at our center. CONCLUSIONS: Few patients remain free of betaherpesviruses after liver transplantation. Most patients presenting active infection with more than one virus were infected sequentially and not concurrently. Nested-polymerase chain reaction can be considered of limited value for clinically monitoring cytomegalovirus and human herpesvirus 6. |
| first_indexed | 2024-04-13T11:57:17Z |
| format | Article |
| id | doaj.art-919451bcc64b4bf9b78088f8b919cc05 |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-04-13T11:57:17Z |
| publishDate | 2011-01-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-919451bcc64b4bf9b78088f8b919cc052022-12-22T02:47:53ZengElsevier EspañaClinics1807-59321980-53222011-01-0166694995310.1590/S1807-59322011000600005Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-upFernanda Aparecida CostaMarcelo Naoki SokiPaula Durante AndradeSandra Helena Alves BononRonaldo Luis ThomasiniAna Maria SampaioMarcelo de Carvalho RamosClaudio Lúcio RossiTeresa Cristina CavalcantiIlka de Fatima BoinMarília LeonardLuiz Sérgio LeonardRaquel Bello StucchiSandra Cecília Botelho CostaOBJECTIVE: The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the clinical status of patients undergoing liver transplant. INTRODUCTION: The human β-herpesviruses, including cytomegalovirus and human herpesvirus 6, are ubiquitous among human populations. Active infections of human herpesvirus 6 and cytomegalovirus are common after liver transplantation, possibly induced and facilitated by allograft rejection and immunosuppressive therapy. Both viruses affect the success of the transplant procedure. METHODS: Thirty patients submitted to liver transplant at the Liver Transplant Unit, at the Gastro Center, State University of Campinas, SP, Brazil, were studied prospectively from six months to one year, nested-polymerase chain reaction for cytomegalovirus and human herpesvirus 6 DNA detections. Two or more consecutive positive nested-polymerase chain reaction were considered indicative of active infection. RESULTS: Active infection by cytomegalovirus was detected in 13/30 (43.3%) patients, median time to first cytomegalovirus detection was 29 days after transplantation (range: 0-99 days). Active infection by human herpesvirus 6 was detected in 12/30 (40%) patients, median time to first human herpesvirus 6 detection was 23.5 days after transplantation (range: 0-273 days). The time-related appearance of each virus was not statistically different (p = 0.49). Rejection of the transplanted liver was observed in 16.7% (5/30) of the patients. The present analysis showed that human herpesvirus 6 and/or cytomegalovirus active infections were frequent in liver transplant recipients at our center. CONCLUSIONS: Few patients remain free of betaherpesviruses after liver transplantation. Most patients presenting active infection with more than one virus were infected sequentially and not concurrently. Nested-polymerase chain reaction can be considered of limited value for clinically monitoring cytomegalovirus and human herpesvirus 6.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000600005CMVHHV-6Nested-PCRLiver transplant |
| spellingShingle | Fernanda Aparecida Costa Marcelo Naoki Soki Paula Durante Andrade Sandra Helena Alves Bonon Ronaldo Luis Thomasini Ana Maria Sampaio Marcelo de Carvalho Ramos Claudio Lúcio Rossi Teresa Cristina Cavalcanti Ilka de Fatima Boin Marília Leonard Luiz Sérgio Leonard Raquel Bello Stucchi Sandra Cecília Botelho Costa Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up Clinics CMV HHV-6 Nested-PCR Liver transplant |
| title | Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up |
| title_full | Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up |
| title_fullStr | Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up |
| title_full_unstemmed | Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up |
| title_short | Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up |
| title_sort | simultaneous monitoring of cmv and human herpesvirus 6 infections and diseases in liver transplant patients one year follow up |
| topic | CMV HHV-6 Nested-PCR Liver transplant |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000600005 |
| work_keys_str_mv | AT fernandaaparecidacosta simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT marcelonaokisoki simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT pauladuranteandrade simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT sandrahelenaalvesbonon simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT ronaldoluisthomasini simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT anamariasampaio simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT marcelodecarvalhoramos simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT claudioluciorossi simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT teresacristinacavalcanti simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT ilkadefatimaboin simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT marilialeonard simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT luizsergioleonard simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT raquelbellostucchi simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup AT sandraceciliabotelhocosta simultaneousmonitoringofcmvandhumanherpesvirus6infectionsanddiseasesinlivertransplantpatientsoneyearfollowup |