FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION
Subject: to assess a role of factors of innate and adaptive immunity in the development of intrauterine generalized cytomegalovirus infection.The study included 47 newborns with congenital generalized cytomegalovirus infection comprising group I. Based on the data of clinical and laboratory examinat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2021-10-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/2420 |
| _version_ | 1797197821067132928 |
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| author | M. A. Levkovich L. V. Kravchenko A. A. Afonin I. I. Krukier A. Yu. Levkovich A. A. Nikashina |
| author_facet | M. A. Levkovich L. V. Kravchenko A. A. Afonin I. I. Krukier A. Yu. Levkovich A. A. Nikashina |
| author_sort | M. A. Levkovich |
| collection | DOAJ |
| description | Subject: to assess a role of factors of innate and adaptive immunity in the development of intrauterine generalized cytomegalovirus infection.The study included 47 newborns with congenital generalized cytomegalovirus infection comprising group I. Based on the data of clinical and laboratory examination, all newborns studied were divided into two subgroups. Subgroup 1.1 (29 subjects) consisted of newborns with severe CMVI and subgroup 1.2 (18 subjects) – newborns with moderate CMVI. The control group included 26 newborns without herpesvirus infection.Determination of the number of monocytes expressing Toll receptors (TLR) was performed by laser flow cytometry (Beckman Coulter) using Beckman Coulter, HyCultBiotechnology reagents: FITC-CD282+, CD284+, CD286+, and PE-CD14+. The newborn serum concentration of IFNγ, IFNα, IL-6, IL-8 was determined by ELISA using BenderMedsistems test systems.Intrauterine generalized CMVI with complete clinical symptoms in newborns was characterized by a decrease in the number of monocytes expressing TLR-2 and TLR-6, which was associated with a decrease in the level of IFNα, IFNγ, an increase in the level of IL-6, IL-8 and MCP-1. The subgroup with incomplete clinical symptoms CMVI was characterized by a decrease in the level of IFNα, in combination with an increase in the level of IL-6. The identified immune disorders lead to a reduction in the antiviral immune response and determine the severity of the disease in prenatally infected newborns. |
| first_indexed | 2024-03-08T05:47:57Z |
| format | Article |
| id | doaj.art-9194eb9b6ebe441f9d06efaa304afb39 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:50:03Z |
| publishDate | 2021-10-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-9194eb9b6ebe441f9d06efaa304afb392024-04-22T13:07:47ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2021-10-0123479980410.15789/1563-0625-FOC-23031447FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTIONM. A. Levkovich0L. V. Kravchenko1A. A. Afonin2I. I. Krukier3A. Yu. Levkovich4A. A. Nikashina5Rostov State Medical University, Research Institute of Obstetrics and PediatricsRostov State Medical University, Research Institute of Obstetrics and PediatricsRostov State Medical University, Research Institute of Obstetrics and PediatricsRostov State Medical University, Research Institute of Obstetrics and PediatricsRegional Clinical Hospital No 2Rostov State Medical University, Research Institute of Obstetrics and PediatricsSubject: to assess a role of factors of innate and adaptive immunity in the development of intrauterine generalized cytomegalovirus infection.The study included 47 newborns with congenital generalized cytomegalovirus infection comprising group I. Based on the data of clinical and laboratory examination, all newborns studied were divided into two subgroups. Subgroup 1.1 (29 subjects) consisted of newborns with severe CMVI and subgroup 1.2 (18 subjects) – newborns with moderate CMVI. The control group included 26 newborns without herpesvirus infection.Determination of the number of monocytes expressing Toll receptors (TLR) was performed by laser flow cytometry (Beckman Coulter) using Beckman Coulter, HyCultBiotechnology reagents: FITC-CD282+, CD284+, CD286+, and PE-CD14+. The newborn serum concentration of IFNγ, IFNα, IL-6, IL-8 was determined by ELISA using BenderMedsistems test systems.Intrauterine generalized CMVI with complete clinical symptoms in newborns was characterized by a decrease in the number of monocytes expressing TLR-2 and TLR-6, which was associated with a decrease in the level of IFNα, IFNγ, an increase in the level of IL-6, IL-8 and MCP-1. The subgroup with incomplete clinical symptoms CMVI was characterized by a decrease in the level of IFNα, in combination with an increase in the level of IL-6. The identified immune disorders lead to a reduction in the antiviral immune response and determine the severity of the disease in prenatally infected newborns.https://www.mimmun.ru/mimmun/article/view/2420innate immunitytlr receptorscytokinesintrauterine infectioncytomegalovirusnewborn children |
| spellingShingle | M. A. Levkovich L. V. Kravchenko A. A. Afonin I. I. Krukier A. Yu. Levkovich A. A. Nikashina FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION Медицинская иммунология innate immunity tlr receptors cytokines intrauterine infection cytomegalovirus newborn children |
| title | FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION |
| title_full | FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION |
| title_fullStr | FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION |
| title_full_unstemmed | FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION |
| title_short | FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION |
| title_sort | factors of congenital and adaptive immunity in the pathogenesis of intrauterine generated cytomegalovirus infection |
| topic | innate immunity tlr receptors cytokines intrauterine infection cytomegalovirus newborn children |
| url | https://www.mimmun.ru/mimmun/article/view/2420 |
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