| Summary: | A combination of organic and conservation approaches have not been widely tested, neither considering agronomic implications nor the impacts on the environment. Focussing on the effect of agricultural practices on greenhouse gas (GHG) emissions from soil, the hypothesis of this research is that the organic conservation system (ORG+) may reduce emissions of N<sub>2</sub>O, CH<sub>4</sub> and CO<sub>2</sub> from soil, compared to an integrated farming system (INT) and an organic (ORG) system in a two-year irrigated vegetable crop rotation set up in 2014, in a Mediterranean environment. The crop rotation included: Savoy cabbage (<i>Brassica oleracea</i> var. sabauda L. cv. Famosa), spring lettuce (<i>Lactuca sativa</i> L. cv. Justine), fennel (<i>Foeniculum vulgare</i> Mill. cv. Montebianco) and summer lettuce (<i>L. sativa</i> cv. Ballerina). Fluxes from soil of N<sub>2</sub>O, CH<sub>4</sub> and CO<sub>2</sub> were measured from October 2014 to July 2016 with the flow-through non-steady state chamber technique using a mobile instrument equipped with high precision analysers. Both cumulative and daily N<sub>2</sub>O emissions were mainly lower in ORG+ than in INT and ORG. All the cropping systems acted as a sink of CH<sub>4</sub>, with no significant differences among treatments. The ORG and ORG+ systems accounted for higher cumulative and daily CO<sub>2</sub> emissions than INT, maybe due to the stimulating effect on soil respiration of organic material (fertilizers/plant biomass) supplied in ORG and ORG+. Overall, the integration of conservation and organic agriculture showed a tendency for higher CO<sub>2</sub> emissions and lower N<sub>2</sub>O emissions than the other treatments, without any clear results on its potential for mitigating GHG emissions from soil.
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