Screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systems
Objectives The widely promising applications of graphene nanomaterials raise considerable concerns regarding their environmental and human health risk assessment. The aim of the current study was to evaluate the toxicity profiling of graphene family nananomaterials (GFNs) in alternative in vitro and...
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| Format: | Article |
| Language: | English |
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Korean Society of Environmental Health and Toxicology
2015-07-01
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| Series: | Environmental Health and Toxicology |
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| Online Access: | http://www.e-eht.org/upload/pdf/eht-30-e2015007.pdf |
| _version_ | 1819095076026974208 |
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| author | Nivedita Chatterjee Ji Su Yang Kwangsik Park Seung Min Oh Jeonggue Park Jinhee Choi |
| author_facet | Nivedita Chatterjee Ji Su Yang Kwangsik Park Seung Min Oh Jeonggue Park Jinhee Choi |
| author_sort | Nivedita Chatterjee |
| collection | DOAJ |
| description | Objectives The widely promising applications of graphene nanomaterials raise considerable concerns regarding their environmental and human health risk assessment. The aim of the current study was to evaluate the toxicity profiling of graphene family nananomaterials (GFNs) in alternative in vitro and in vivo toxicity testing models. Methods The GFNs used in this study are graphene nanoplatelets ([GNPs]–pristine, carboxylate [COOH] and amide [NH2]) and graphene oxides (single layer [SLGO] and few layers [FLGO]). The human bronchial epithelial cells (Beas2B cells) as in vitro system and the nematode Caenorhabditis elegans as in vivo system were used to profile the toxicity response of GFNs. Cytotoxicity assays, colony formation assay for cellular toxicity and reproduction potentiality in C. elegans were used as end points to evaluate the GFNs’ toxicity. Results In general, GNPs exhibited higher toxicity than GOs in Beas2B cells, and among the GNPs the order of toxicity was pristine>NH2>COOH. Although the order of toxicity of the GNPs was maintained in C. elegans reproductive toxicity, but GOs were found to be more toxic in the worms than GNPs. In both systems, SLGO exhibited profoundly greater dose dependency than FLGO. The possible reason of their differential toxicity lay in their distinctive physicochemical characteristics and agglomeration behavior in the exposure media. Conclusions The present study revealed that the toxicity of GFNs is dependent on the graphene nanomaterial’s physical forms, surface functionalizations, number of layers, dose, time of exposure and obviously, on the alternative model systems used for toxicity assessment. |
| first_indexed | 2024-12-21T23:37:33Z |
| format | Article |
| id | doaj.art-919a98cb0a474804a2e8657df43de52b |
| institution | Directory Open Access Journal |
| issn | 2233-6567 |
| language | English |
| last_indexed | 2024-12-21T23:37:33Z |
| publishDate | 2015-07-01 |
| publisher | Korean Society of Environmental Health and Toxicology |
| record_format | Article |
| series | Environmental Health and Toxicology |
| spelling | doaj.art-919a98cb0a474804a2e8657df43de52b2022-12-21T18:46:20ZengKorean Society of Environmental Health and ToxicologyEnvironmental Health and Toxicology2233-65672015-07-013010.5620/eht.e2015007729Screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systemsNivedita Chatterjee0Ji Su Yang1Kwangsik Park2Seung Min Oh3Jeonggue Park4Jinhee Choi5 School of Environmental Engineering, Graduate School of Energy and Environmental System Engineering, University of Seoul, Seoul, Korea School of Environmental Engineering, Graduate School of Energy and Environmental System Engineering, University of Seoul, Seoul, Korea College of Pharmacy, Dongduk Women’s University, Seoul, Korea Fusion Technology Laboratory, Hoseo University, Asan, Korea Korea Environmental Institute, Seoul, Korea School of Environmental Engineering, Graduate School of Energy and Environmental System Engineering, University of Seoul, Seoul, KoreaObjectives The widely promising applications of graphene nanomaterials raise considerable concerns regarding their environmental and human health risk assessment. The aim of the current study was to evaluate the toxicity profiling of graphene family nananomaterials (GFNs) in alternative in vitro and in vivo toxicity testing models. Methods The GFNs used in this study are graphene nanoplatelets ([GNPs]–pristine, carboxylate [COOH] and amide [NH2]) and graphene oxides (single layer [SLGO] and few layers [FLGO]). The human bronchial epithelial cells (Beas2B cells) as in vitro system and the nematode Caenorhabditis elegans as in vivo system were used to profile the toxicity response of GFNs. Cytotoxicity assays, colony formation assay for cellular toxicity and reproduction potentiality in C. elegans were used as end points to evaluate the GFNs’ toxicity. Results In general, GNPs exhibited higher toxicity than GOs in Beas2B cells, and among the GNPs the order of toxicity was pristine>NH2>COOH. Although the order of toxicity of the GNPs was maintained in C. elegans reproductive toxicity, but GOs were found to be more toxic in the worms than GNPs. In both systems, SLGO exhibited profoundly greater dose dependency than FLGO. The possible reason of their differential toxicity lay in their distinctive physicochemical characteristics and agglomeration behavior in the exposure media. Conclusions The present study revealed that the toxicity of GFNs is dependent on the graphene nanomaterial’s physical forms, surface functionalizations, number of layers, dose, time of exposure and obviously, on the alternative model systems used for toxicity assessment.http://www.e-eht.org/upload/pdf/eht-30-e2015007.pdfAlternative toxicity testingHuman bronchial epithelial cellsGraphene family nanomaterials |
| spellingShingle | Nivedita Chatterjee Ji Su Yang Kwangsik Park Seung Min Oh Jeonggue Park Jinhee Choi Screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systems Environmental Health and Toxicology Alternative toxicity testing Human bronchial epithelial cells Graphene family nanomaterials |
| title | Screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systems |
| title_full | Screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systems |
| title_fullStr | Screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systems |
| title_full_unstemmed | Screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systems |
| title_short | Screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systems |
| title_sort | screening of toxic potential of graphene family nanomaterials using and alternative toxicity testing systems |
| topic | Alternative toxicity testing Human bronchial epithelial cells Graphene family nanomaterials |
| url | http://www.e-eht.org/upload/pdf/eht-30-e2015007.pdf |
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