A standardised method of marking male mosquitoes with fluorescent dust
Abstract Background Prior to a major release campaign of sterile insects, including the sterile insect technique, male mosquitoes must be marked and released (small scale) to determine key parameters including wild population abundance, dispersal and survival. Marking insects has been routinely carr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-020-04066-6 |
| _version_ | 1818325572084826112 |
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| author | Nicole J. Culbert Maria Kaiser Nelius Venter Marc J. B. Vreysen Jeremie R. L. Gilles Jérémy Bouyer |
| author_facet | Nicole J. Culbert Maria Kaiser Nelius Venter Marc J. B. Vreysen Jeremie R. L. Gilles Jérémy Bouyer |
| author_sort | Nicole J. Culbert |
| collection | DOAJ |
| description | Abstract Background Prior to a major release campaign of sterile insects, including the sterile insect technique, male mosquitoes must be marked and released (small scale) to determine key parameters including wild population abundance, dispersal and survival. Marking insects has been routinely carried out for over 100 years; however, there is no gold standard regarding the marking of specific disease-transmitting mosquitoes including Anopheles arabiensis, Aedes aegypti and Aedes albopictus. The research presented offers a novel dusting technique and optimal dust colour and quantities, suitable for small-scale releases, such as mark-release-recapture studies. Methods We sought to establish a suitable dust colour and quantity for batches of 100 male An. arabiensis, that was visible both by eye and under UV light, long-lasting and did not negatively impact longevity. A set of lower dust weights were selected to conduct longevity experiments with both Ae. aegypti and Ae. albopictus to underpin the optimal dust weight. A further study assessed the potential of marked male An. arabiensis to transfer their mark to undusted males and females. Results The longevity of male An. arabiensis marked with various dust colours was not significantly reduced when compared to unmarked controls. Furthermore, the chosen dust quantity (5 mg) did not negatively impact longevity (P = 0.717) and provided a long-lasting mark. Dust transfer was found to occur from marked An. arabiensis males to unmarked males and females when left in close proximity. However, this was only noticeable when examining individuals under a stereomicroscope and thus deemed negligible. Overall, male Ae. aegypti and Ae. albopictus displayed a greater sensitivity to dusting. Only the lowest dust weight (0.5 mg) did not significantly reduce longevity (P = 0.888) in Ae. aegypti, whilst the lowest two dust weights (0.5 and 0.75 mg) had no significant impact on longevity (P = 0.951 and 0.166, respectively) in Ae. albopictus. Conclusion We have devised a fast, inexpensive and simple marking method and provided recommended dust quantities for several major species of disease-causing mosquitoes. The novel technique provides an evenly distributed, long-lasting mark which is non-detrimental. Our results will be useful for future MRR studies, prior to a major release campaign. |
| first_indexed | 2024-12-13T11:46:36Z |
| format | Article |
| id | doaj.art-919d7c1e22464d75971150f1c81fe3d5 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-13T11:46:36Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-919d7c1e22464d75971150f1c81fe3d52022-12-21T23:47:29ZengBMCParasites & Vectors1756-33052020-04-0113111110.1186/s13071-020-04066-6A standardised method of marking male mosquitoes with fluorescent dustNicole J. Culbert0Maria Kaiser1Nelius Venter2Marc J. B. Vreysen3Jeremie R. L. Gilles4Jérémy Bouyer5Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and AgricultureVector Control Reference Unit, National Institute for Communicable Diseases of the National Health Laboratory ServiceVector Control Reference Unit, National Institute for Communicable Diseases of the National Health Laboratory ServiceInsect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and AgricultureInsect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and AgricultureInsect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and AgricultureAbstract Background Prior to a major release campaign of sterile insects, including the sterile insect technique, male mosquitoes must be marked and released (small scale) to determine key parameters including wild population abundance, dispersal and survival. Marking insects has been routinely carried out for over 100 years; however, there is no gold standard regarding the marking of specific disease-transmitting mosquitoes including Anopheles arabiensis, Aedes aegypti and Aedes albopictus. The research presented offers a novel dusting technique and optimal dust colour and quantities, suitable for small-scale releases, such as mark-release-recapture studies. Methods We sought to establish a suitable dust colour and quantity for batches of 100 male An. arabiensis, that was visible both by eye and under UV light, long-lasting and did not negatively impact longevity. A set of lower dust weights were selected to conduct longevity experiments with both Ae. aegypti and Ae. albopictus to underpin the optimal dust weight. A further study assessed the potential of marked male An. arabiensis to transfer their mark to undusted males and females. Results The longevity of male An. arabiensis marked with various dust colours was not significantly reduced when compared to unmarked controls. Furthermore, the chosen dust quantity (5 mg) did not negatively impact longevity (P = 0.717) and provided a long-lasting mark. Dust transfer was found to occur from marked An. arabiensis males to unmarked males and females when left in close proximity. However, this was only noticeable when examining individuals under a stereomicroscope and thus deemed negligible. Overall, male Ae. aegypti and Ae. albopictus displayed a greater sensitivity to dusting. Only the lowest dust weight (0.5 mg) did not significantly reduce longevity (P = 0.888) in Ae. aegypti, whilst the lowest two dust weights (0.5 and 0.75 mg) had no significant impact on longevity (P = 0.951 and 0.166, respectively) in Ae. albopictus. Conclusion We have devised a fast, inexpensive and simple marking method and provided recommended dust quantities for several major species of disease-causing mosquitoes. The novel technique provides an evenly distributed, long-lasting mark which is non-detrimental. Our results will be useful for future MRR studies, prior to a major release campaign.http://link.springer.com/article/10.1186/s13071-020-04066-6Sterile insect techniqueDustDipteraMark-release-recapture |
| spellingShingle | Nicole J. Culbert Maria Kaiser Nelius Venter Marc J. B. Vreysen Jeremie R. L. Gilles Jérémy Bouyer A standardised method of marking male mosquitoes with fluorescent dust Parasites & Vectors Sterile insect technique Dust Diptera Mark-release-recapture |
| title | A standardised method of marking male mosquitoes with fluorescent dust |
| title_full | A standardised method of marking male mosquitoes with fluorescent dust |
| title_fullStr | A standardised method of marking male mosquitoes with fluorescent dust |
| title_full_unstemmed | A standardised method of marking male mosquitoes with fluorescent dust |
| title_short | A standardised method of marking male mosquitoes with fluorescent dust |
| title_sort | standardised method of marking male mosquitoes with fluorescent dust |
| topic | Sterile insect technique Dust Diptera Mark-release-recapture |
| url | http://link.springer.com/article/10.1186/s13071-020-04066-6 |
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