Improved xylose and arabinose utilization by an industrial recombinant <it>Saccharomyces cerevisiae </it>strain using evolutionary engineering
<p>Abstract</p> <p>Background</p> <p>Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by <it>Saccharomyces cerevisiae </it>requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-06-01
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| Series: | Biotechnology for Biofuels |
| Online Access: | http://www.biotechnologyforbiofuels.com/content/3/1/13 |
| _version_ | 1818200388373839872 |
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| author | Almeida João RM Sànchez Nogué Violeta Fonseca César Karhumaa Kaisa Garcia Sanchez Rosa Larsson Christer U Bengtsson Oskar Bettiga Maurizio Hahn-Hägerdal Bärbel Gorwa-Grauslund Marie F |
| author_facet | Almeida João RM Sànchez Nogué Violeta Fonseca César Karhumaa Kaisa Garcia Sanchez Rosa Larsson Christer U Bengtsson Oskar Bettiga Maurizio Hahn-Hägerdal Bärbel Gorwa-Grauslund Marie F |
| author_sort | Almeida João RM |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by <it>Saccharomyces cerevisiae </it>requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to ethanol must be enhanced.</p> <p>Results</p> <p>Evolutionary engineering was used to improve the simultaneous conversion of xylose and arabinose to ethanol in a recombinant industrial <it>Saccharomyces cerevisiae </it>strain carrying the heterologous genes for xylose and arabinose utilization pathways integrated in the genome. The evolved strain TMB3130 displayed an increased consumption rate of xylose and arabinose under aerobic and anaerobic conditions. Improved anaerobic ethanol production was achieved at the expense of xylitol and glycerol but arabinose was almost stoichiometrically converted to arabitol. Further characterization of the strain indicated that the selection pressure during prolonged continuous culture in xylose and arabinose medium resulted in the improved transport of xylose and arabinose as well as increased levels of the enzymes from the introduced fungal xylose pathway. No mutation was found in any of the genes from the pentose converting pathways.</p> <p>Conclusion</p> <p>To the best of our knowledge, this is the first report that characterizes the molecular mechanisms for improved mixed-pentose utilization obtained by evolutionary engineering of a recombinant <it>S. cerevisiae </it>strain. Increased transport of pentoses and increased activities of xylose converting enzymes contributed to the improved phenotype.</p> |
| first_indexed | 2024-12-12T02:36:52Z |
| format | Article |
| id | doaj.art-91a27b85922a4d95b1b1140c7aaaa87f |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T02:36:52Z |
| publishDate | 2010-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-91a27b85922a4d95b1b1140c7aaaa87f2022-12-22T00:41:16ZengBMCBiotechnology for Biofuels1754-68342010-06-01311310.1186/1754-6834-3-13Improved xylose and arabinose utilization by an industrial recombinant <it>Saccharomyces cerevisiae </it>strain using evolutionary engineeringAlmeida João RMSànchez Nogué VioletaFonseca CésarKarhumaa KaisaGarcia Sanchez RosaLarsson Christer UBengtsson OskarBettiga MaurizioHahn-Hägerdal BärbelGorwa-Grauslund Marie F<p>Abstract</p> <p>Background</p> <p>Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by <it>Saccharomyces cerevisiae </it>requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to ethanol must be enhanced.</p> <p>Results</p> <p>Evolutionary engineering was used to improve the simultaneous conversion of xylose and arabinose to ethanol in a recombinant industrial <it>Saccharomyces cerevisiae </it>strain carrying the heterologous genes for xylose and arabinose utilization pathways integrated in the genome. The evolved strain TMB3130 displayed an increased consumption rate of xylose and arabinose under aerobic and anaerobic conditions. Improved anaerobic ethanol production was achieved at the expense of xylitol and glycerol but arabinose was almost stoichiometrically converted to arabitol. Further characterization of the strain indicated that the selection pressure during prolonged continuous culture in xylose and arabinose medium resulted in the improved transport of xylose and arabinose as well as increased levels of the enzymes from the introduced fungal xylose pathway. No mutation was found in any of the genes from the pentose converting pathways.</p> <p>Conclusion</p> <p>To the best of our knowledge, this is the first report that characterizes the molecular mechanisms for improved mixed-pentose utilization obtained by evolutionary engineering of a recombinant <it>S. cerevisiae </it>strain. Increased transport of pentoses and increased activities of xylose converting enzymes contributed to the improved phenotype.</p>http://www.biotechnologyforbiofuels.com/content/3/1/13 |
| spellingShingle | Almeida João RM Sànchez Nogué Violeta Fonseca César Karhumaa Kaisa Garcia Sanchez Rosa Larsson Christer U Bengtsson Oskar Bettiga Maurizio Hahn-Hägerdal Bärbel Gorwa-Grauslund Marie F Improved xylose and arabinose utilization by an industrial recombinant <it>Saccharomyces cerevisiae </it>strain using evolutionary engineering Biotechnology for Biofuels |
| title | Improved xylose and arabinose utilization by an industrial recombinant <it>Saccharomyces cerevisiae </it>strain using evolutionary engineering |
| title_full | Improved xylose and arabinose utilization by an industrial recombinant <it>Saccharomyces cerevisiae </it>strain using evolutionary engineering |
| title_fullStr | Improved xylose and arabinose utilization by an industrial recombinant <it>Saccharomyces cerevisiae </it>strain using evolutionary engineering |
| title_full_unstemmed | Improved xylose and arabinose utilization by an industrial recombinant <it>Saccharomyces cerevisiae </it>strain using evolutionary engineering |
| title_short | Improved xylose and arabinose utilization by an industrial recombinant <it>Saccharomyces cerevisiae </it>strain using evolutionary engineering |
| title_sort | improved xylose and arabinose utilization by an industrial recombinant it saccharomyces cerevisiae it strain using evolutionary engineering |
| url | http://www.biotechnologyforbiofuels.com/content/3/1/13 |
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