miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails
Objective(s): Erythropoiesis is regulated by some extrinsic and intrinsic factors as microRNAs (miRNAs). miRNAs are endogenously small non-coding regulatory RNAs which play vital roles in the variety of cellular fate, critical processes; growth, apoptosis, metabolism, survival of the cells and speci...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2013-06-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_991_1076303710387490212667f5c4ad7ccb.html |
| _version_ | 1818791495051771904 |
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| author | Fatemeh Kouhkan Masoud Soleimani Morteza Daliri Majid Mossahebi-mohammadi Shahin Mohammadi Mehdi Mokhtari Reyhaneh Lahmy naser Mobarra Mehrdad Behmanesh |
| author_facet | Fatemeh Kouhkan Masoud Soleimani Morteza Daliri Majid Mossahebi-mohammadi Shahin Mohammadi Mehdi Mokhtari Reyhaneh Lahmy naser Mobarra Mehrdad Behmanesh |
| author_sort | Fatemeh Kouhkan |
| collection | DOAJ |
| description | Objective(s): Erythropoiesis is regulated by some extrinsic and intrinsic factors as microRNAs (miRNAs). miRNAs are endogenously small non-coding regulatory RNAs which play vital roles in the variety of cellular fate, critical processes; growth, apoptosis, metabolism, survival of the cells and specially differentiation. Several miRNAs such as miR-16 and miR-451 have been shown to be correlated with erythroid differentiation. Taking into account the importance of miRNAs in cellular differentiation, the goal of the present study was to examine the role of miRNAs in hematopoietic stem cells (HSC) differentiation into the erythroid cells in the absence of growth factors and stimulatory cytokines. Materials and Methods: CD133+ stem cells were infected with lentiviruses containing miR-451/miR-16 precursor sequence, erythroid differentiation was evaluated using RT-PCR for hemoglobin chains and surface antigens, also by banzidine staining. Results: MiR-451up-regulation, but not miR-16, could induce α, β and γ-globin expression in CD133+ cells and have strong correlation with appearance of CD71 and CD235a markers in these cells. Moreover, miR-451 up-regulation increases the banzidine positive cells to ~ %40. Conclusion: Our results provide strong evidence that miR-451 up-regulation strongly induces erythroid differentiation and maturation of CD133+ stem cells. Hence, this method may provide a useful technique for the production of artificial blood RBC and be used as a new strategy for gene therapy of hemoglobinopathies, such as β-thalassemias and sickle cell anemia. |
| first_indexed | 2024-12-18T15:12:15Z |
| format | Article |
| id | doaj.art-91a3f4a2f27b439091ba4f579ac7d6e7 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T15:12:15Z |
| publishDate | 2013-06-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-91a3f4a2f27b439091ba4f579ac7d6e72022-12-21T21:03:37ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742013-06-01166756762991miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine CocktailsFatemeh Kouhkan0Masoud Soleimani1Morteza Daliri2Majid Mossahebi-mohammadi3Shahin Mohammadi4Mehdi Mokhtari5Reyhaneh Lahmy6naser Mobarra7Mehrdad Behmanesh8Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, IranDepartment of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, IranNational institute of genetic engineering and biotechnology, Tehran, IranDepartment of Clinical biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, IranDepartments of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, IranDepartment of Clinical biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, IranObjective(s): Erythropoiesis is regulated by some extrinsic and intrinsic factors as microRNAs (miRNAs). miRNAs are endogenously small non-coding regulatory RNAs which play vital roles in the variety of cellular fate, critical processes; growth, apoptosis, metabolism, survival of the cells and specially differentiation. Several miRNAs such as miR-16 and miR-451 have been shown to be correlated with erythroid differentiation. Taking into account the importance of miRNAs in cellular differentiation, the goal of the present study was to examine the role of miRNAs in hematopoietic stem cells (HSC) differentiation into the erythroid cells in the absence of growth factors and stimulatory cytokines. Materials and Methods: CD133+ stem cells were infected with lentiviruses containing miR-451/miR-16 precursor sequence, erythroid differentiation was evaluated using RT-PCR for hemoglobin chains and surface antigens, also by banzidine staining. Results: MiR-451up-regulation, but not miR-16, could induce α, β and γ-globin expression in CD133+ cells and have strong correlation with appearance of CD71 and CD235a markers in these cells. Moreover, miR-451 up-regulation increases the banzidine positive cells to ~ %40. Conclusion: Our results provide strong evidence that miR-451 up-regulation strongly induces erythroid differentiation and maturation of CD133+ stem cells. Hence, this method may provide a useful technique for the production of artificial blood RBC and be used as a new strategy for gene therapy of hemoglobinopathies, such as β-thalassemias and sickle cell anemia.http://ijbms.mums.ac.ir/pdf_991_1076303710387490212667f5c4ad7ccb.htmlErythropoiesis CD133+ microRNA miR-451 miR-16 |
| spellingShingle | Fatemeh Kouhkan Masoud Soleimani Morteza Daliri Majid Mossahebi-mohammadi Shahin Mohammadi Mehdi Mokhtari Reyhaneh Lahmy naser Mobarra Mehrdad Behmanesh miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails Iranian Journal of Basic Medical Sciences Erythropoiesis CD133+ microRNA miR-451 miR-16 |
| title | miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails |
| title_full | miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails |
| title_fullStr | miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails |
| title_full_unstemmed | miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails |
| title_short | miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails |
| title_sort | mir 451 up regulation induce erythroid differentiation of cd133 cells independent of cytokine cocktails |
| topic | Erythropoiesis CD133+ microRNA miR-451 miR-16 |
| url | http://ijbms.mums.ac.ir/pdf_991_1076303710387490212667f5c4ad7ccb.html |
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