Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in Kerman
Background & Aims: Esophageal Cancer is the sixth fatal cancer in the world. Squamous and adenocarcinoma account for 95% of esophageal cancer. The expression of EGFR has a role in the pathophysiology of epidermal-based malignancies such as esophageal cancer. EGFR is also an important criterion i...
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2012-07-01
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| Series: | Journal of Kerman University of Medical Sciences |
| Subjects: | |
| Online Access: | https://jkmu.kmu.ac.ir/article_16524_8c328c69e1bf3522cdb074f9094550e9.pdf |
| _version_ | 1797790627241394176 |
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| author | M.R Lashkarizadeh M.R Bazrafshani M Aghaei-Afshar Neda Zahiri S Dehghan-Kohestani |
| author_facet | M.R Lashkarizadeh M.R Bazrafshani M Aghaei-Afshar Neda Zahiri S Dehghan-Kohestani |
| author_sort | M.R Lashkarizadeh |
| collection | DOAJ |
| description | Background & Aims: Esophageal Cancer is the sixth fatal cancer in the world. Squamous and adenocarcinoma account for 95% of esophageal cancer. The expression of EGFR has a role in the pathophysiology of epidermal-based malignancies such as esophageal cancer. EGFR is also an important criterion in the evaluation of disease staging and prognosis. The aim of this study was to survey the prevalence of EGFR gene mutations in patients with esophageal cancer by MLPA Technique. Methods: A total of 60 parafinated blocks from patients with esophageal cancer were investigated for the presence of EGFR mutations by MLPA technique. Results: EGFR mutation was discovered in 82 percent of samples of which 52% were deletion mostly seen in exon 2 (52%) and duplication mostly seen in exon 27 (54%). In some cases deletion and/or duplication were seen in more than one exon simultaneously. Conclusion: With regard to the obtained results and since the definitive diagnosis of esophageal cancer is made by biopsy and pathology techniques, it is suggested that all biopsy specimens be detected for EGFR mutations, particularly on exons 2 and 27 in order to achieve a noninvasive molecular diagnostic technique in future. |
| first_indexed | 2024-03-13T02:07:16Z |
| format | Article |
| id | doaj.art-91a4206a4ca14c3a960acf0f10d9edbc |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:07:16Z |
| publishDate | 2012-07-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-91a4206a4ca14c3a960acf0f10d9edbc2023-07-01T05:28:42ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432012-07-0119325325916524Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in KermanM.R Lashkarizadeh0M.R Bazrafshani1M Aghaei-Afshar2Neda Zahiri3S Dehghan-Kohestani4Assistant Professor of Surgery, Afzalipour School of Medicine & Physiology Research Center, Kerman University of Medical Sciences, Kerman, IranAssistant Professor of Medical Genetics, Afzalipour School of Medicine & Physiology Research Center, Kerman University of Medical Sciences, Kerman, IranAssistant Professor of Surgery, Afzalipour School of Medicine & Physiology Research Center, Kerman University of Medical Sciences, Kerman, IranSurgeon, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, IranLaboratory Staff, Dr. Bazrafshani’s Medical Genetic Laboratory, Kerman, IranBackground & Aims: Esophageal Cancer is the sixth fatal cancer in the world. Squamous and adenocarcinoma account for 95% of esophageal cancer. The expression of EGFR has a role in the pathophysiology of epidermal-based malignancies such as esophageal cancer. EGFR is also an important criterion in the evaluation of disease staging and prognosis. The aim of this study was to survey the prevalence of EGFR gene mutations in patients with esophageal cancer by MLPA Technique. Methods: A total of 60 parafinated blocks from patients with esophageal cancer were investigated for the presence of EGFR mutations by MLPA technique. Results: EGFR mutation was discovered in 82 percent of samples of which 52% were deletion mostly seen in exon 2 (52%) and duplication mostly seen in exon 27 (54%). In some cases deletion and/or duplication were seen in more than one exon simultaneously. Conclusion: With regard to the obtained results and since the definitive diagnosis of esophageal cancer is made by biopsy and pathology techniques, it is suggested that all biopsy specimens be detected for EGFR mutations, particularly on exons 2 and 27 in order to achieve a noninvasive molecular diagnostic technique in future.https://jkmu.kmu.ac.ir/article_16524_8c328c69e1bf3522cdb074f9094550e9.pdfesophageal neoplasmegfrmutationmlpa |
| spellingShingle | M.R Lashkarizadeh M.R Bazrafshani M Aghaei-Afshar Neda Zahiri S Dehghan-Kohestani Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in Kerman Journal of Kerman University of Medical Sciences esophageal neoplasm egfr mutation mlpa |
| title | Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in Kerman |
| title_full | Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in Kerman |
| title_fullStr | Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in Kerman |
| title_full_unstemmed | Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in Kerman |
| title_short | Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in Kerman |
| title_sort | prevalence of nucleotide alterations of egfr gene in patients with esophageal squamous cell carcinoma in kerman |
| topic | esophageal neoplasm egfr mutation mlpa |
| url | https://jkmu.kmu.ac.ir/article_16524_8c328c69e1bf3522cdb074f9094550e9.pdf |
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