Deciphering the CircRNA-Regulated Response of Western Honey Bee (<i>Apis mellifera</i>) Workers to Microsporidian Invasion
<i>Vairimorpha ceranae</i> is a widespread fungal parasite of adult honey bees that leads to a serious disease called nosemosis. Circular RNAs (circRNAs) are newly discovered non-coding RNAs (ncRNAs) that regulate biological processes such as immune defense and development. Here, 8199 an...
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MDPI AG
2022-08-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/9/1285 |
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| author | Huazhi Chen Xiaoxue Fan Wende Zhang Yaping Ye Zongbing Cai Kaiyao Zhang Kuihao Zhang Zhongmin Fu Dafu Chen Rui Guo |
| author_facet | Huazhi Chen Xiaoxue Fan Wende Zhang Yaping Ye Zongbing Cai Kaiyao Zhang Kuihao Zhang Zhongmin Fu Dafu Chen Rui Guo |
| author_sort | Huazhi Chen |
| collection | DOAJ |
| description | <i>Vairimorpha ceranae</i> is a widespread fungal parasite of adult honey bees that leads to a serious disease called nosemosis. Circular RNAs (circRNAs) are newly discovered non-coding RNAs (ncRNAs) that regulate biological processes such as immune defense and development. Here, 8199 and 8711 circRNAs were predicted from the midguts of <i>Apis mellifera ligustica</i> workers at 7 d (Am7T) and 10 d (Am10T) after inoculation (dpi) with <i>V. ceranae</i> spores. In combination with transcriptome data from corresponding uninoculated midguts (Am7CK and Am10CK), 4464 circRNAs were found to be shared by these four groups. Additionally, 16 circRNAs were highly conserved among <i>A. m. ligustica</i>, <i>Apis cerana cerana</i>, and <i>Homo sapiens</i>. In the Am7CK vs. Am7T (Am10CK vs. Am10T) comparison group, 168 (306) differentially expressed circRNAs (DEcircRNAs) were identified. RT-qPCR results showed that the expression trend of eight DEcircRNAs was consistent with that in the transcriptome datasets. The source genes of DEcircRNAs in Am7CK vs. Am7T (Am10CK vs. Am10T) were engaged in 27 (35) GO functional terms, including 1 (1) immunity-associated terms. Moreover, the aforementioned source genes were involved in three cellular immune-related pathways. Moreover, 86 (178) DEcircRNAs in workers’ midguts at 7 (10) dpi could interact with 75 (103) miRNAs, further targeting 215 (305) mRNAs. These targets were associated with cellular renewal, cellular structure, carbohydrate and energy metabolism, and cellular and humoral immunity. Findings in the present study unraveled the mechanism underlying circRNA-mediated immune responses of western honey bee workers to <i>V. ceranae</i> invasion, but also provided new insights into host–microsporidian interaction during nosemosis. |
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| spelling | doaj.art-91a4f97df0a646a187c247df1d89cb232023-11-23T15:06:58ZengMDPI AGBiology2079-77372022-08-01119128510.3390/biology11091285Deciphering the CircRNA-Regulated Response of Western Honey Bee (<i>Apis mellifera</i>) Workers to Microsporidian InvasionHuazhi Chen0Xiaoxue Fan1Wende Zhang2Yaping Ye3Zongbing Cai4Kaiyao Zhang5Kuihao Zhang6Zhongmin Fu7Dafu Chen8Rui Guo9College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, ChinaCollege of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 35002, China<i>Vairimorpha ceranae</i> is a widespread fungal parasite of adult honey bees that leads to a serious disease called nosemosis. Circular RNAs (circRNAs) are newly discovered non-coding RNAs (ncRNAs) that regulate biological processes such as immune defense and development. Here, 8199 and 8711 circRNAs were predicted from the midguts of <i>Apis mellifera ligustica</i> workers at 7 d (Am7T) and 10 d (Am10T) after inoculation (dpi) with <i>V. ceranae</i> spores. In combination with transcriptome data from corresponding uninoculated midguts (Am7CK and Am10CK), 4464 circRNAs were found to be shared by these four groups. Additionally, 16 circRNAs were highly conserved among <i>A. m. ligustica</i>, <i>Apis cerana cerana</i>, and <i>Homo sapiens</i>. In the Am7CK vs. Am7T (Am10CK vs. Am10T) comparison group, 168 (306) differentially expressed circRNAs (DEcircRNAs) were identified. RT-qPCR results showed that the expression trend of eight DEcircRNAs was consistent with that in the transcriptome datasets. The source genes of DEcircRNAs in Am7CK vs. Am7T (Am10CK vs. Am10T) were engaged in 27 (35) GO functional terms, including 1 (1) immunity-associated terms. Moreover, the aforementioned source genes were involved in three cellular immune-related pathways. Moreover, 86 (178) DEcircRNAs in workers’ midguts at 7 (10) dpi could interact with 75 (103) miRNAs, further targeting 215 (305) mRNAs. These targets were associated with cellular renewal, cellular structure, carbohydrate and energy metabolism, and cellular and humoral immunity. Findings in the present study unraveled the mechanism underlying circRNA-mediated immune responses of western honey bee workers to <i>V. ceranae</i> invasion, but also provided new insights into host–microsporidian interaction during nosemosis.https://www.mdpi.com/2079-7737/11/9/1285western honey beecircular RNA<i>Vairimorpha ceranae</i>noncoding RNAhost–pathogen interactionimmune response |
| spellingShingle | Huazhi Chen Xiaoxue Fan Wende Zhang Yaping Ye Zongbing Cai Kaiyao Zhang Kuihao Zhang Zhongmin Fu Dafu Chen Rui Guo Deciphering the CircRNA-Regulated Response of Western Honey Bee (<i>Apis mellifera</i>) Workers to Microsporidian Invasion Biology western honey bee circular RNA <i>Vairimorpha ceranae</i> noncoding RNA host–pathogen interaction immune response |
| title | Deciphering the CircRNA-Regulated Response of Western Honey Bee (<i>Apis mellifera</i>) Workers to Microsporidian Invasion |
| title_full | Deciphering the CircRNA-Regulated Response of Western Honey Bee (<i>Apis mellifera</i>) Workers to Microsporidian Invasion |
| title_fullStr | Deciphering the CircRNA-Regulated Response of Western Honey Bee (<i>Apis mellifera</i>) Workers to Microsporidian Invasion |
| title_full_unstemmed | Deciphering the CircRNA-Regulated Response of Western Honey Bee (<i>Apis mellifera</i>) Workers to Microsporidian Invasion |
| title_short | Deciphering the CircRNA-Regulated Response of Western Honey Bee (<i>Apis mellifera</i>) Workers to Microsporidian Invasion |
| title_sort | deciphering the circrna regulated response of western honey bee i apis mellifera i workers to microsporidian invasion |
| topic | western honey bee circular RNA <i>Vairimorpha ceranae</i> noncoding RNA host–pathogen interaction immune response |
| url | https://www.mdpi.com/2079-7737/11/9/1285 |
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