Efficacy of 405 nm Light-Emitting Diode Illumination and Citral Used Alone and in Combination for Inactivation of <i>Vibrio parahaemolyticus</i> on Shrimp
<i>Vibrio parahaemolyticus</i> is a widely distributed pathogen, which is frequently the lead cause of infections related to seafood consumption. The objective of the present study was to investigate the antimicrobial effect of the combination of 405 nm light-emitting diode (LED) and cit...
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MDPI AG
2022-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/14/2008 |
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| author | Yingying Zhang Shuo Wang Du Guo Zhiyuan Liu Jianxue Gao Xiangjun Zhan Yutang Wang Chao Shi Xiaodong Xia |
| author_facet | Yingying Zhang Shuo Wang Du Guo Zhiyuan Liu Jianxue Gao Xiangjun Zhan Yutang Wang Chao Shi Xiaodong Xia |
| author_sort | Yingying Zhang |
| collection | DOAJ |
| description | <i>Vibrio parahaemolyticus</i> is a widely distributed pathogen, which is frequently the lead cause of infections related to seafood consumption. The objective of the present study was to investigate the antimicrobial effect of the combination of 405 nm light-emitting diode (LED) and citral on <i>V. parahaemolyticus</i>. The antimicrobial effect of LED illumination and citral was evaluated on <i>V. parahaemolyticus</i> not only in phosphate-buffered saline (PBS) but also on shrimp. Quality changes of shrimp were determined by sensory evaluation. Changes in bacteria cell membrane morphology, cell membrane permeability, cell lipid oxidation level, and DNA degradation were examined to provide insights into the antimicrobial mechanism. The combination of LED treatments and citral had better antimicrobial effects than either treatment alone. LED combined with 0.1 mg/mL of citral effectively reduced <i>V. parahaemolyticus</i> from 6.5 log CFU/mL to below the detection limit in PBS. Combined treatment caused a 3.5 log reduction of the pathogen on shrimp within 20 min and a 6 log reduction within 2 h without significant changes in the sensory score. Furthermore, combined LED and citral treatment affected <i>V. parahaemolyticus</i> cellular morphology and outer membrane integrity. The profile of the comet assay and DNA fragmentation analysis revealed that combination treatment did not cause a breakdown of bacterial genomic DNA. In conclusion, LED may act synergistically with citral. They have the potential to be developed as novel microbial intervention strategies. |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T11:54:39Z |
| publishDate | 2022-07-01 |
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| series | Foods |
| spelling | doaj.art-91a52a4c8c944dcfbb42b164f582ca102023-11-30T23:10:29ZengMDPI AGFoods2304-81582022-07-011114200810.3390/foods11142008Efficacy of 405 nm Light-Emitting Diode Illumination and Citral Used Alone and in Combination for Inactivation of <i>Vibrio parahaemolyticus</i> on ShrimpYingying Zhang0Shuo Wang1Du Guo2Zhiyuan Liu3Jianxue Gao4Xiangjun Zhan5Yutang Wang6Chao Shi7Xiaodong Xia8College of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, China<i>Vibrio parahaemolyticus</i> is a widely distributed pathogen, which is frequently the lead cause of infections related to seafood consumption. The objective of the present study was to investigate the antimicrobial effect of the combination of 405 nm light-emitting diode (LED) and citral on <i>V. parahaemolyticus</i>. The antimicrobial effect of LED illumination and citral was evaluated on <i>V. parahaemolyticus</i> not only in phosphate-buffered saline (PBS) but also on shrimp. Quality changes of shrimp were determined by sensory evaluation. Changes in bacteria cell membrane morphology, cell membrane permeability, cell lipid oxidation level, and DNA degradation were examined to provide insights into the antimicrobial mechanism. The combination of LED treatments and citral had better antimicrobial effects than either treatment alone. LED combined with 0.1 mg/mL of citral effectively reduced <i>V. parahaemolyticus</i> from 6.5 log CFU/mL to below the detection limit in PBS. Combined treatment caused a 3.5 log reduction of the pathogen on shrimp within 20 min and a 6 log reduction within 2 h without significant changes in the sensory score. Furthermore, combined LED and citral treatment affected <i>V. parahaemolyticus</i> cellular morphology and outer membrane integrity. The profile of the comet assay and DNA fragmentation analysis revealed that combination treatment did not cause a breakdown of bacterial genomic DNA. In conclusion, LED may act synergistically with citral. They have the potential to be developed as novel microbial intervention strategies.https://www.mdpi.com/2304-8158/11/14/2008405 nm LED<i>Vibrio parahaemolyticus</i>citralshrimpcell membrane |
| spellingShingle | Yingying Zhang Shuo Wang Du Guo Zhiyuan Liu Jianxue Gao Xiangjun Zhan Yutang Wang Chao Shi Xiaodong Xia Efficacy of 405 nm Light-Emitting Diode Illumination and Citral Used Alone and in Combination for Inactivation of <i>Vibrio parahaemolyticus</i> on Shrimp Foods 405 nm LED <i>Vibrio parahaemolyticus</i> citral shrimp cell membrane |
| title | Efficacy of 405 nm Light-Emitting Diode Illumination and Citral Used Alone and in Combination for Inactivation of <i>Vibrio parahaemolyticus</i> on Shrimp |
| title_full | Efficacy of 405 nm Light-Emitting Diode Illumination and Citral Used Alone and in Combination for Inactivation of <i>Vibrio parahaemolyticus</i> on Shrimp |
| title_fullStr | Efficacy of 405 nm Light-Emitting Diode Illumination and Citral Used Alone and in Combination for Inactivation of <i>Vibrio parahaemolyticus</i> on Shrimp |
| title_full_unstemmed | Efficacy of 405 nm Light-Emitting Diode Illumination and Citral Used Alone and in Combination for Inactivation of <i>Vibrio parahaemolyticus</i> on Shrimp |
| title_short | Efficacy of 405 nm Light-Emitting Diode Illumination and Citral Used Alone and in Combination for Inactivation of <i>Vibrio parahaemolyticus</i> on Shrimp |
| title_sort | efficacy of 405 nm light emitting diode illumination and citral used alone and in combination for inactivation of i vibrio parahaemolyticus i on shrimp |
| topic | 405 nm LED <i>Vibrio parahaemolyticus</i> citral shrimp cell membrane |
| url | https://www.mdpi.com/2304-8158/11/14/2008 |
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