UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in <i>Astragalus mongholicus</i> Seedlings
<b>: </b>Ultraviolet-B (UV-B) radiation (280−320 nm) may induce photobiological stress in plants, activate the plant defense system, and induce changes of metabolites. In our previous work, we found that between the two <i>Astragalus</i> varieties prescribed by the...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-03-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/10/4/504 |
| _version_ | 1818176886794092544 |
|---|---|
| author | Yang Liu Jia Liu Ann Abozeid Ke-Xin Wu Xiao-Rui Guo Li-Qiang Mu Zhong-Hua Tang |
| author_facet | Yang Liu Jia Liu Ann Abozeid Ke-Xin Wu Xiao-Rui Guo Li-Qiang Mu Zhong-Hua Tang |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | <b>: </b>Ultraviolet-B (UV-B) radiation (280−320 nm) may induce photobiological stress in plants, activate the plant defense system, and induce changes of metabolites. In our previous work, we found that between the two <i>Astragalus</i> varieties prescribed by the Chinese Pharmacopoeia, <i>Astragalus mongholicus</i> has better tolerance to UV-B. Thus, it is necessary to study the metabolic strategy of <i>Astragalus </i>under UV-B radiation further. In the present study, we used untargeted gas chromatography-mass spectrometry (GC-MS) and targeted liquid chromatography-mass spectrometry (LC-MS techniques) to investigate the profiles of primary and secondary metabolic. The profiles revealed the metabolic response of<i> Astragalus </i>to UV-B radiation. We then used real-time polymerase chain reaction (RT-PCR) to obtain the transcription level of relevant genes under UV-B radiation (UV-B supplemented in the field, λ<sub>max</sub> = 313 nm, 30 W, lamp-leaf distance = 60 cm, 40 min·day<sup>-1</sup>), which annotated the responsive mechanism of phenolic metabolism in roots. Our results indicated that supplemental UV-B radiation induced a stronger shift from carbon assimilation to carbon accumulation. The flux through the phenylpropanoids pathway increased due to the mobilization of carbon reserves. The response of metabolism was observed to be significantly tissue-specific upon the UV-B radiation treatment. Among phenolic compounds, C6C1 carbon compounds (phenolic acids in leaves) and C6C3C6 carbon compounds (flavones in leaves and isoflavones in roots) increased at the expense of C6C3 carbon compounds. Verification experiments show that the response of phenolics in roots to UV-B is activated by upregulation of relevant genes rather than phenylalanine. Overall, this study reveals the tissues-specific alteration and mechanism of primary and secondary metabolic strategy in response to UV-B radiation. |
| first_indexed | 2024-12-11T20:23:19Z |
| format | Article |
| id | doaj.art-91a694e593d44e49b331211cb8259ef2 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-11T20:23:19Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-91a694e593d44e49b331211cb8259ef22022-12-22T00:52:01ZengMDPI AGBiomolecules2218-273X2020-03-0110450410.3390/biom10040504biom10040504UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in <i>Astragalus mongholicus</i> SeedlingsYang Liu0Jia Liu1Ann Abozeid2Ke-Xin Wu3Xiao-Rui Guo4Li-Qiang Mu5Zhong-Hua Tang6School of Forestry, Northeast Forestry University, Harbin 150040, ChinaMaterial Science and Engineering College, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, ChinaSchool of Forestry, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China<b>: </b>Ultraviolet-B (UV-B) radiation (280−320 nm) may induce photobiological stress in plants, activate the plant defense system, and induce changes of metabolites. In our previous work, we found that between the two <i>Astragalus</i> varieties prescribed by the Chinese Pharmacopoeia, <i>Astragalus mongholicus</i> has better tolerance to UV-B. Thus, it is necessary to study the metabolic strategy of <i>Astragalus </i>under UV-B radiation further. In the present study, we used untargeted gas chromatography-mass spectrometry (GC-MS) and targeted liquid chromatography-mass spectrometry (LC-MS techniques) to investigate the profiles of primary and secondary metabolic. The profiles revealed the metabolic response of<i> Astragalus </i>to UV-B radiation. We then used real-time polymerase chain reaction (RT-PCR) to obtain the transcription level of relevant genes under UV-B radiation (UV-B supplemented in the field, λ<sub>max</sub> = 313 nm, 30 W, lamp-leaf distance = 60 cm, 40 min·day<sup>-1</sup>), which annotated the responsive mechanism of phenolic metabolism in roots. Our results indicated that supplemental UV-B radiation induced a stronger shift from carbon assimilation to carbon accumulation. The flux through the phenylpropanoids pathway increased due to the mobilization of carbon reserves. The response of metabolism was observed to be significantly tissue-specific upon the UV-B radiation treatment. Among phenolic compounds, C6C1 carbon compounds (phenolic acids in leaves) and C6C3C6 carbon compounds (flavones in leaves and isoflavones in roots) increased at the expense of C6C3 carbon compounds. Verification experiments show that the response of phenolics in roots to UV-B is activated by upregulation of relevant genes rather than phenylalanine. Overall, this study reveals the tissues-specific alteration and mechanism of primary and secondary metabolic strategy in response to UV-B radiation.https://www.mdpi.com/2218-273X/10/4/504astragalus mongholicusultraviolet-b radiationphenolicsuntargeted gas chromatography-mass spectrometrytargeted liquid chromatography-mass spectrometry |
| spellingShingle | Yang Liu Jia Liu Ann Abozeid Ke-Xin Wu Xiao-Rui Guo Li-Qiang Mu Zhong-Hua Tang UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in <i>Astragalus mongholicus</i> Seedlings Biomolecules astragalus mongholicus ultraviolet-b radiation phenolics untargeted gas chromatography-mass spectrometry targeted liquid chromatography-mass spectrometry |
| title | UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in <i>Astragalus mongholicus</i> Seedlings |
| title_full | UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in <i>Astragalus mongholicus</i> Seedlings |
| title_fullStr | UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in <i>Astragalus mongholicus</i> Seedlings |
| title_full_unstemmed | UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in <i>Astragalus mongholicus</i> Seedlings |
| title_short | UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in <i>Astragalus mongholicus</i> Seedlings |
| title_sort | uv b radiation largely promoted the transformation of primary metabolites to phenols in i astragalus mongholicus i seedlings |
| topic | astragalus mongholicus ultraviolet-b radiation phenolics untargeted gas chromatography-mass spectrometry targeted liquid chromatography-mass spectrometry |
| url | https://www.mdpi.com/2218-273X/10/4/504 |
| work_keys_str_mv | AT yangliu uvbradiationlargelypromotedthetransformationofprimarymetabolitestophenolsiniastragalusmongholicusiseedlings AT jialiu uvbradiationlargelypromotedthetransformationofprimarymetabolitestophenolsiniastragalusmongholicusiseedlings AT annabozeid uvbradiationlargelypromotedthetransformationofprimarymetabolitestophenolsiniastragalusmongholicusiseedlings AT kexinwu uvbradiationlargelypromotedthetransformationofprimarymetabolitestophenolsiniastragalusmongholicusiseedlings AT xiaoruiguo uvbradiationlargelypromotedthetransformationofprimarymetabolitestophenolsiniastragalusmongholicusiseedlings AT liqiangmu uvbradiationlargelypromotedthetransformationofprimarymetabolitestophenolsiniastragalusmongholicusiseedlings AT zhonghuatang uvbradiationlargelypromotedthetransformationofprimarymetabolitestophenolsiniastragalusmongholicusiseedlings |