A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research
Novel binary gene expression tools like the LexA-LexAop system could powerfully enhance studies of metabolism, development, and neurobiology in Drosophila. However, specific LexA drivers for neuroendocrine cells and many other developmentally relevant systems remain limited. In a unique high school...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2016-10-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.116.031229 |
| _version_ | 1818864441081462784 |
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| author | Lutz Kockel Lutfi M. Huq Anika Ayyar Emma Herold Elle MacAlpine Madeline Logan Christina Savvides Grace E. S. Kim Jiapei Chen Theresa Clark Trang Duong Vahid Fazel-Rezai Deanna Havey Samuel Han Ravi Jagadeesan Eun Soo Jackie Kim Diane Lee Kaelina Lombardo Ida Piyale Hansen Shi Lydia Stahr Dana Tung Uriel Tayvah Flora Wang Ja-Hon Wang Sarah Xiao Sydni M. Topper Sangbin Park Cheryl Rotondo Anne E. Rankin Townley W. Chisholm Seung K. Kim |
| author_facet | Lutz Kockel Lutfi M. Huq Anika Ayyar Emma Herold Elle MacAlpine Madeline Logan Christina Savvides Grace E. S. Kim Jiapei Chen Theresa Clark Trang Duong Vahid Fazel-Rezai Deanna Havey Samuel Han Ravi Jagadeesan Eun Soo Jackie Kim Diane Lee Kaelina Lombardo Ida Piyale Hansen Shi Lydia Stahr Dana Tung Uriel Tayvah Flora Wang Ja-Hon Wang Sarah Xiao Sydni M. Topper Sangbin Park Cheryl Rotondo Anne E. Rankin Townley W. Chisholm Seung K. Kim |
| author_sort | Lutz Kockel |
| collection | DOAJ |
| description | Novel binary gene expression tools like the LexA-LexAop system could powerfully enhance studies of metabolism, development, and neurobiology in Drosophila. However, specific LexA drivers for neuroendocrine cells and many other developmentally relevant systems remain limited. In a unique high school biology course, we generated a LexA-based enhancer trap collection by transposon mobilization. The initial collection provides a source of novel LexA-based elements that permit targeted gene expression in the corpora cardiaca, cells central for metabolic homeostasis, and other neuroendocrine cell types. The collection further contains specific LexA drivers for stem cells and other enteric cells in the gut, and other developmentally relevant tissue types. We provide detailed analysis of nearly 100 new LexA lines, including molecular mapping of insertions, description of enhancer-driven reporter expression in larval tissues, and adult neuroendocrine cells, comparison with established enhancer trap collections and tissue specific RNAseq. Generation of this open-resource LexA collection facilitates neuroendocrine and developmental biology investigations, and shows how empowering secondary school science can achieve research and educational goals. |
| first_indexed | 2024-12-19T10:31:42Z |
| format | Article |
| id | doaj.art-91a8b8a4f9664a33ad9a6f0f5010ef3e |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-19T10:31:42Z |
| publishDate | 2016-10-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-91a8b8a4f9664a33ad9a6f0f5010ef3e2022-12-21T20:25:44ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362016-10-016103017302610.1534/g3.116.0312292A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine ResearchLutz KockelLutfi M. HuqAnika AyyarEmma HeroldElle MacAlpineMadeline LoganChristina SavvidesGrace E. S. KimJiapei ChenTheresa ClarkTrang DuongVahid Fazel-RezaiDeanna HaveySamuel HanRavi JagadeesanEun Soo Jackie KimDiane LeeKaelina LombardoIda PiyaleHansen ShiLydia StahrDana TungUriel TayvahFlora WangJa-Hon WangSarah XiaoSydni M. TopperSangbin ParkCheryl RotondoAnne E. RankinTownley W. ChisholmSeung K. KimNovel binary gene expression tools like the LexA-LexAop system could powerfully enhance studies of metabolism, development, and neurobiology in Drosophila. However, specific LexA drivers for neuroendocrine cells and many other developmentally relevant systems remain limited. In a unique high school biology course, we generated a LexA-based enhancer trap collection by transposon mobilization. The initial collection provides a source of novel LexA-based elements that permit targeted gene expression in the corpora cardiaca, cells central for metabolic homeostasis, and other neuroendocrine cell types. The collection further contains specific LexA drivers for stem cells and other enteric cells in the gut, and other developmentally relevant tissue types. We provide detailed analysis of nearly 100 new LexA lines, including molecular mapping of insertions, description of enhancer-driven reporter expression in larval tissues, and adult neuroendocrine cells, comparison with established enhancer trap collections and tissue specific RNAseq. Generation of this open-resource LexA collection facilitates neuroendocrine and developmental biology investigations, and shows how empowering secondary school science can achieve research and educational goals.http://g3journal.org/lookup/doi/10.1534/g3.116.031229Drosophila melanogasterDrosophila neuro-endocrine system enhancer trapLexA-LexAop binary expression system |
| spellingShingle | Lutz Kockel Lutfi M. Huq Anika Ayyar Emma Herold Elle MacAlpine Madeline Logan Christina Savvides Grace E. S. Kim Jiapei Chen Theresa Clark Trang Duong Vahid Fazel-Rezai Deanna Havey Samuel Han Ravi Jagadeesan Eun Soo Jackie Kim Diane Lee Kaelina Lombardo Ida Piyale Hansen Shi Lydia Stahr Dana Tung Uriel Tayvah Flora Wang Ja-Hon Wang Sarah Xiao Sydni M. Topper Sangbin Park Cheryl Rotondo Anne E. Rankin Townley W. Chisholm Seung K. Kim A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research G3: Genes, Genomes, Genetics Drosophila melanogaster Drosophila neuro-endocrine system enhancer trap LexA-LexAop binary expression system |
| title | A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research |
| title_full | A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research |
| title_fullStr | A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research |
| title_full_unstemmed | A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research |
| title_short | A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research |
| title_sort | drosophila lexa enhancer trap resource for developmental biology and neuroendocrine research |
| topic | Drosophila melanogaster Drosophila neuro-endocrine system enhancer trap LexA-LexAop binary expression system |
| url | http://g3journal.org/lookup/doi/10.1534/g3.116.031229 |
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