DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35
Abstract Background Colorectal cancer (CRC) has become the second deadliest cancer in the world and severely threatens human health. An increasing number of studies have focused on the role of the RNA helicase DEAD-box (DDX) family in CRC. However, the mechanism of DDX10 in CRC has not been elucidat...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-02-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-022-02478-1 |
| _version_ | 1798022590479990784 |
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| author | Xin Zhou Zhihong Liu Tengfei He Cuifeng Zhang Manman Jiang Yuxiao Jin Ziyu Wu Changji Gu Wei Zhang Xiaodong Yang |
| author_facet | Xin Zhou Zhihong Liu Tengfei He Cuifeng Zhang Manman Jiang Yuxiao Jin Ziyu Wu Changji Gu Wei Zhang Xiaodong Yang |
| author_sort | Xin Zhou |
| collection | DOAJ |
| description | Abstract Background Colorectal cancer (CRC) has become the second deadliest cancer in the world and severely threatens human health. An increasing number of studies have focused on the role of the RNA helicase DEAD-box (DDX) family in CRC. However, the mechanism of DDX10 in CRC has not been elucidated. Methods In our study, we analysed the expression data of CRC samples from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Subsequently, we performed cytological experiments and animal experiments to explore the role of DDX10 in CRC cells. Furthermore, we performed Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and protein–protein interaction (PPI) network analyses. Finally, we predicted the interacting protein of DDX10 by LC–MS/MS and verified it by coimmunoprecipitation (Co-IP) and qPCR. Results In the present study, we identified that DDX10 mRNA was extremely highly expressed in CRC tissues compared with normal colon tissues in the TCGA and GEO databases. The protein expression of DDX10 was measured by immunochemistry (IHC) in 17 CRC patients. The biological roles of DDX10 were explored via cell and molecular biology experiments in vitro and in vivo and cell cycle assays. We found that DDX10 knockdown markedly reduced CRC cell proliferation, migration and invasion. Then, we constructed a PPI network with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING). GO and KEGG enrichment analysis and gene set enrichment analysis (GSEA) showed that DDX10 was closely related to RNA splicing and E2F targets. Using LC–MS/MS and Co-IP assays, we discovered that RPL35 is the interacting protein of DDX10. In addition, we hypothesize that RPL35 is related to the E2F pathway and the immune response in CRC. Conclusions In conclusion, provides a better understanding of the molecular mechanisms of DDX10 in CRC and provides a potential biomarker for the diagnosis and treatment of CRC. |
| first_indexed | 2024-04-11T17:32:27Z |
| format | Article |
| id | doaj.art-91aa1356bd0f4f7cb9ba99e02684a15a |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-11T17:32:27Z |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-91aa1356bd0f4f7cb9ba99e02684a15a2022-12-22T04:11:58ZengBMCCancer Cell International1475-28672022-02-0122111210.1186/s12935-022-02478-1DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35Xin Zhou0Zhihong Liu1Tengfei He2Cuifeng Zhang3Manman Jiang4Yuxiao Jin5Ziyu Wu6Changji Gu7Wei Zhang8Xiaodong Yang9Department of General Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of General Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of General Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of General Surgery, The Second Affiliated Hospital of Soochow UniversitySoochow UniversityDepartment of General Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of General Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of General Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of Radiotherapy, The Second Affiliated Hospital of Soochow UniversityDepartment of General Surgery, The Second Affiliated Hospital of Soochow UniversityAbstract Background Colorectal cancer (CRC) has become the second deadliest cancer in the world and severely threatens human health. An increasing number of studies have focused on the role of the RNA helicase DEAD-box (DDX) family in CRC. However, the mechanism of DDX10 in CRC has not been elucidated. Methods In our study, we analysed the expression data of CRC samples from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Subsequently, we performed cytological experiments and animal experiments to explore the role of DDX10 in CRC cells. Furthermore, we performed Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and protein–protein interaction (PPI) network analyses. Finally, we predicted the interacting protein of DDX10 by LC–MS/MS and verified it by coimmunoprecipitation (Co-IP) and qPCR. Results In the present study, we identified that DDX10 mRNA was extremely highly expressed in CRC tissues compared with normal colon tissues in the TCGA and GEO databases. The protein expression of DDX10 was measured by immunochemistry (IHC) in 17 CRC patients. The biological roles of DDX10 were explored via cell and molecular biology experiments in vitro and in vivo and cell cycle assays. We found that DDX10 knockdown markedly reduced CRC cell proliferation, migration and invasion. Then, we constructed a PPI network with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING). GO and KEGG enrichment analysis and gene set enrichment analysis (GSEA) showed that DDX10 was closely related to RNA splicing and E2F targets. Using LC–MS/MS and Co-IP assays, we discovered that RPL35 is the interacting protein of DDX10. In addition, we hypothesize that RPL35 is related to the E2F pathway and the immune response in CRC. Conclusions In conclusion, provides a better understanding of the molecular mechanisms of DDX10 in CRC and provides a potential biomarker for the diagnosis and treatment of CRC.https://doi.org/10.1186/s12935-022-02478-1Colorectal cancerDEAD-box helicase 10 (DDX10)OncogeneRPL35mRNA splicing |
| spellingShingle | Xin Zhou Zhihong Liu Tengfei He Cuifeng Zhang Manman Jiang Yuxiao Jin Ziyu Wu Changji Gu Wei Zhang Xiaodong Yang DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35 Cancer Cell International Colorectal cancer DEAD-box helicase 10 (DDX10) Oncogene RPL35 mRNA splicing |
| title | DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35 |
| title_full | DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35 |
| title_fullStr | DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35 |
| title_full_unstemmed | DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35 |
| title_short | DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35 |
| title_sort | ddx10 promotes the proliferation and metastasis of colorectal cancer cells via splicing rpl35 |
| topic | Colorectal cancer DEAD-box helicase 10 (DDX10) Oncogene RPL35 mRNA splicing |
| url | https://doi.org/10.1186/s12935-022-02478-1 |
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