Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells
BACKGROUND: Dental pulp stem cell (DPSC) and periodontal ligament stem cell (PDLSC) have been suggested as valuable seed cells for bone engineering, suggesting that both stem cells are potential osteogenic sources. Since DPSC and PDLSC seem like to have similar potential in bone formation, we conduc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Secretariat of The Indonesian Biomedical Journal
2017-08-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/286 |
| _version_ | 1819054703453929472 |
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| author | Ferry Sandra Janti Sudiono Ciptadhi Tri Oka Binartha Angliana Chouw Melanie Sadono Djamil |
| author_facet | Ferry Sandra Janti Sudiono Ciptadhi Tri Oka Binartha Angliana Chouw Melanie Sadono Djamil |
| author_sort | Ferry Sandra |
| collection | DOAJ |
| description | BACKGROUND: Dental pulp stem cell (DPSC) and periodontal ligament stem cell (PDLSC) have been suggested as valuable seed cells for bone engineering, suggesting that both stem cells are potential osteogenic sources. Since DPSC and PDLSC seem like to have similar potential in bone formation, we conducted a study to compare morphology, immunophenotype and cell growth of DPSC and PDLSC isolated from the same teeth.
METHODS: Human dental pulps and periodontal ligaments were obtained from freshly extracted partial impacted third molar teeth. Collected samples were digested with type I collagenase. Resulted cell suspension was washed and cultured. For biomarker identification, the cells were fixed and bound with anti-fluorescein isothiocyanate (FITC)-cluster of differentiation (CD)117 antibody. For cell growth quantification, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used. Meanwhile for osteogenic differentiation, the cells were cultured in osteogenic medium for 1-3 weeks, fixed and stained with alizarin red.
RESULTS: Morphology of dental pulps cell (DPC) and periodontal ligament cell (PDLC) in passage 5 was similar. Clear CD117 green fluorescence of DPC and PDLC in passage 5 was observed. Cell growth rate of PDLC was higher than the one of DPC, 0.3858 and 0.3848 respectively. DPC formed bone nodule on the third week culture in osteogenic medium, while PDLC showed bone nodule formation on the second week culture.
CONCLUSION: We suggest that DPC and PDLC are potential seed cells for osteogenic regeneration, since they had cell growth capacity and osteogenic differentiation, particularly PDLC that had faster osteogenic differentiation.
KEYWORDS: dental pulp, periodontal ligament, cell, growth, osteogenic differentiation |
| first_indexed | 2024-12-21T12:55:50Z |
| format | Article |
| id | doaj.art-91aef4a2cc75417aa7717037a0e8e8e1 |
| institution | Directory Open Access Journal |
| issn | 2085-3297 2355-9179 |
| language | English |
| last_indexed | 2024-12-21T12:55:50Z |
| publishDate | 2017-08-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj.art-91aef4a2cc75417aa7717037a0e8e8e12022-12-21T19:03:20ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792017-08-0192788310.18585/inabj.v9i2.286239Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament CellsFerry Sandra0Janti Sudiono1Ciptadhi Tri Oka Binartha2Angliana Chouw3Melanie Sadono Djamil4Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Trisakti University, Jl. Kyai Tapa No.260, JakartaDepartment of Oral Pathology, Division of Oral Biology, Faculty of Dentistry, Trisakti University, Jl. Kyai Tapa No.260, JakartaDepartment of Microbiology, Division of Oral Biology, Faculty of Dentistry, Trisakti University, Jl. Kyai Tapa No.260, JakartaProdia Stemcell Indonesia, Jl. Kramat VII No.11, JakartaDepartment of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Trisakti University, Jl. Kyai Tapa No.260, JakartaBACKGROUND: Dental pulp stem cell (DPSC) and periodontal ligament stem cell (PDLSC) have been suggested as valuable seed cells for bone engineering, suggesting that both stem cells are potential osteogenic sources. Since DPSC and PDLSC seem like to have similar potential in bone formation, we conducted a study to compare morphology, immunophenotype and cell growth of DPSC and PDLSC isolated from the same teeth. METHODS: Human dental pulps and periodontal ligaments were obtained from freshly extracted partial impacted third molar teeth. Collected samples were digested with type I collagenase. Resulted cell suspension was washed and cultured. For biomarker identification, the cells were fixed and bound with anti-fluorescein isothiocyanate (FITC)-cluster of differentiation (CD)117 antibody. For cell growth quantification, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used. Meanwhile for osteogenic differentiation, the cells were cultured in osteogenic medium for 1-3 weeks, fixed and stained with alizarin red. RESULTS: Morphology of dental pulps cell (DPC) and periodontal ligament cell (PDLC) in passage 5 was similar. Clear CD117 green fluorescence of DPC and PDLC in passage 5 was observed. Cell growth rate of PDLC was higher than the one of DPC, 0.3858 and 0.3848 respectively. DPC formed bone nodule on the third week culture in osteogenic medium, while PDLC showed bone nodule formation on the second week culture. CONCLUSION: We suggest that DPC and PDLC are potential seed cells for osteogenic regeneration, since they had cell growth capacity and osteogenic differentiation, particularly PDLC that had faster osteogenic differentiation. KEYWORDS: dental pulp, periodontal ligament, cell, growth, osteogenic differentiationhttps://inabj.org/index.php/ibj/article/view/286 |
| spellingShingle | Ferry Sandra Janti Sudiono Ciptadhi Tri Oka Binartha Angliana Chouw Melanie Sadono Djamil Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells Indonesian Biomedical Journal |
| title | Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells |
| title_full | Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells |
| title_fullStr | Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells |
| title_full_unstemmed | Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells |
| title_short | Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells |
| title_sort | growth and osteogenic differentiation of cd117 dental pulp and periodontal ligament cells |
| url | https://inabj.org/index.php/ibj/article/view/286 |
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