Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device
We reported an automated dielectrophoretic (DEP) tweezers-based force spectroscopy system to examine intermolecular weak binding interactions, which consists of three components: (1) interdigitated electrodes and micro-sized polystyrene particles used as DEP tweezers and probes inside a microfluidic...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-10-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/17/10/2272 |
| _version_ | 1798005693056286720 |
|---|---|
| author | Min Hyung Kim Jeongjick Lee Kihwan Nam In Soo Park Myeonggu Son Hyunchul Ko Sangyoup Lee Dae Sung Yoon Woo-Jin Chang Sei Young Lee Young Ro Yoon Sang Woo Lee |
| author_facet | Min Hyung Kim Jeongjick Lee Kihwan Nam In Soo Park Myeonggu Son Hyunchul Ko Sangyoup Lee Dae Sung Yoon Woo-Jin Chang Sei Young Lee Young Ro Yoon Sang Woo Lee |
| author_sort | Min Hyung Kim |
| collection | DOAJ |
| description | We reported an automated dielectrophoretic (DEP) tweezers-based force spectroscopy system to examine intermolecular weak binding interactions, which consists of three components: (1) interdigitated electrodes and micro-sized polystyrene particles used as DEP tweezers and probes inside a microfluidic device, along with an arbitrary function generator connected to a high voltage amplifier; (2) microscopy hooked up to a high-speed charge coupled device (CCD) camera with an image acquisition device; and (3) a computer aid control system based on the LabVIEW program. Using this automated system, we verified the measurement reliability by measuring intermolecular weak binding interactions, such as hydrogen bonds and Van der Waals interactions. In addition, we also observed the linearity of the force loading rates, which is applied to the probes by the DEP tweezers, by varying the number of voltage increment steps and thus affecting the linearity of the force loading rates. This system provides a simple and low-cost platform to investigate intermolecular weak binding interactions. |
| first_indexed | 2024-04-11T12:44:26Z |
| format | Article |
| id | doaj.art-91b0e753dbcc44a5826aef35c7ed0f34 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:44:26Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-91b0e753dbcc44a5826aef35c7ed0f342022-12-22T04:23:25ZengMDPI AGSensors1424-82202017-10-011710227210.3390/s17102272s17102272Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic DeviceMin Hyung Kim0Jeongjick Lee1Kihwan Nam2In Soo Park3Myeonggu Son4Hyunchul Ko5Sangyoup Lee6Dae Sung Yoon7Woo-Jin Chang8Sei Young Lee9Young Ro Yoon10Sang Woo Lee11Department of Biomedical Engineering, Yonsei University, Wonju 220-710, KoreaDepartment of Biomedical Engineering, Yonsei University, Wonju 220-710, KoreaBiomedical Research Institute, Korea Institute of Science and Technology, Seoul 136-791, KoreaDepartment of Biomedical Engineering, Yonsei University, Wonju 220-710, KoreaDepartment of Biomedical Engineering, Yonsei University, Wonju 220-710, KoreaDepartment of Biomedical Engineering, Yonsei University, Wonju 220-710, KoreaBiomedical Research Institute, Korea Institute of Science and Technology, Seoul 136-791, KoreaDepartment of Bio-Convergence, Korea University, Seoul 136-701, KoreaMechanical Engineering Department, University of Wisconsin-Milwaukee, 3200 N Cramer St., Milwaukee, WI 53211, USADepartment of Biomedical Engineering, Yonsei University, Wonju 220-710, KoreaDepartment of Biomedical Engineering, Yonsei University, Wonju 220-710, KoreaDepartment of Biomedical Engineering, Yonsei University, Wonju 220-710, KoreaWe reported an automated dielectrophoretic (DEP) tweezers-based force spectroscopy system to examine intermolecular weak binding interactions, which consists of three components: (1) interdigitated electrodes and micro-sized polystyrene particles used as DEP tweezers and probes inside a microfluidic device, along with an arbitrary function generator connected to a high voltage amplifier; (2) microscopy hooked up to a high-speed charge coupled device (CCD) camera with an image acquisition device; and (3) a computer aid control system based on the LabVIEW program. Using this automated system, we verified the measurement reliability by measuring intermolecular weak binding interactions, such as hydrogen bonds and Van der Waals interactions. In addition, we also observed the linearity of the force loading rates, which is applied to the probes by the DEP tweezers, by varying the number of voltage increment steps and thus affecting the linearity of the force loading rates. This system provides a simple and low-cost platform to investigate intermolecular weak binding interactions.https://www.mdpi.com/1424-8220/17/10/2272dielectrophoresisforce spectroscopyforce loading rateintermolecular weak binding interactions |
| spellingShingle | Min Hyung Kim Jeongjick Lee Kihwan Nam In Soo Park Myeonggu Son Hyunchul Ko Sangyoup Lee Dae Sung Yoon Woo-Jin Chang Sei Young Lee Young Ro Yoon Sang Woo Lee Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device Sensors dielectrophoresis force spectroscopy force loading rate intermolecular weak binding interactions |
| title | Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device |
| title_full | Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device |
| title_fullStr | Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device |
| title_full_unstemmed | Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device |
| title_short | Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device |
| title_sort | automated dielectrophoretic tweezers based force spectroscopy system in a microfluidic device |
| topic | dielectrophoresis force spectroscopy force loading rate intermolecular weak binding interactions |
| url | https://www.mdpi.com/1424-8220/17/10/2272 |
| work_keys_str_mv | AT minhyungkim automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT jeongjicklee automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT kihwannam automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT insoopark automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT myeongguson automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT hyunchulko automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT sangyouplee automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT daesungyoon automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT woojinchang automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT seiyounglee automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT youngroyoon automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice AT sangwoolee automateddielectrophoretictweezersbasedforcespectroscopysysteminamicrofluidicdevice |