Protocol for Single-Molecule Fluorescence In Situ Hybridization for Intact Pancreatic Tissue
Summary: We describe an optimized smFISH protocol for the intact pancreas. The protocol is adapted from Lyubimova et al. (2013), a generic tissue smFISH protocol that works for most tissues but not the pancreas. The main changes implemented include increasing the period of mRNA denaturation from 5 m...
Main Authors: | Lydia Farack, Shalev Itzkovitz |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-06-01
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Series: | STAR Protocols |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166719300073 |
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