Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry
Summary: Coupling of hemodynamic responses to neuronal activity is the foundation of several functional neuroimaging techniques. Here, we provide three fiber-photometry approaches to simultaneously measure neuronal and vascular signals in the rodent brain using a spectrometer-based system. Two out o...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672200377X |
| _version_ | 1811236128836026368 |
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| author | Wei-Ting Zhang Tzu-Hao Harry Chao Guohong Cui Yen-Yu Ian Shih |
| author_facet | Wei-Ting Zhang Tzu-Hao Harry Chao Guohong Cui Yen-Yu Ian Shih |
| author_sort | Wei-Ting Zhang |
| collection | DOAJ |
| description | Summary: Coupling of hemodynamic responses to neuronal activity is the foundation of several functional neuroimaging techniques. Here, we provide three fiber-photometry approaches to simultaneously measure neuronal and vascular signals in the rodent brain using a spectrometer-based system. Two out of these three approaches allow the removal of hemoglobin (Hb)-absorption artifacts and restore the underlying neuronal activity. This technique is applicable to different fluorescent sensors and provides a more accurate measurement of hemodynamic response function in any location of the rodent brain.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-12T12:03:37Z |
| format | Article |
| id | doaj.art-91b272521ca64fb98fde5ea6c26901f1 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-12T12:03:37Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-91b272521ca64fb98fde5ea6c26901f12022-12-22T03:33:47ZengElsevierSTAR Protocols2666-16672022-09-0133101497Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometryWei-Ting Zhang0Tzu-Hao Harry Chao1Guohong Cui2Yen-Yu Ian Shih3Center for Animal MRI, Biomedical Research Imaging Center, Department of Neurology, the University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7025, USA; Corresponding authorCenter for Animal MRI, Biomedical Research Imaging Center, Department of Neurology, the University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7025, USA; Corresponding authorIn vivo Neurobiology Group, Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Durham, NC 27709, USACenter for Animal MRI, Biomedical Research Imaging Center, Department of Neurology, the University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7025, USASummary: Coupling of hemodynamic responses to neuronal activity is the foundation of several functional neuroimaging techniques. Here, we provide three fiber-photometry approaches to simultaneously measure neuronal and vascular signals in the rodent brain using a spectrometer-based system. Two out of these three approaches allow the removal of hemoglobin (Hb)-absorption artifacts and restore the underlying neuronal activity. This technique is applicable to different fluorescent sensors and provides a more accurate measurement of hemodynamic response function in any location of the rodent brain.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672200377XBioinformaticsModel OrganismsNeuroscienceMolecular/Chemical Probes |
| spellingShingle | Wei-Ting Zhang Tzu-Hao Harry Chao Guohong Cui Yen-Yu Ian Shih Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry STAR Protocols Bioinformatics Model Organisms Neuroscience Molecular/Chemical Probes |
| title | Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry |
| title_full | Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry |
| title_fullStr | Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry |
| title_full_unstemmed | Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry |
| title_short | Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry |
| title_sort | simultaneous recording of neuronal and vascular activity in the rodent brain using fiber photometry |
| topic | Bioinformatics Model Organisms Neuroscience Molecular/Chemical Probes |
| url | http://www.sciencedirect.com/science/article/pii/S266616672200377X |
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