Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi Discrete typing units.
Trypanosoma cruzi the agent of Chagas disease is a monophyletic but heterogeneous group conformed by several Discrete Typing Units (DTUs) named TcI to TcVI characterized by genetic markers. The trans-sialidase (TS) is a virulence factor involved in cell invasion and pathogenesis that is differential...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3594200?pdf=render |
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| author | Juan M Burgos Marikena G Risso Simone Frédérique Brenière Christian Barnabé Oscar Campetella María Susana Leguizamón |
| author_facet | Juan M Burgos Marikena G Risso Simone Frédérique Brenière Christian Barnabé Oscar Campetella María Susana Leguizamón |
| author_sort | Juan M Burgos |
| collection | DOAJ |
| description | Trypanosoma cruzi the agent of Chagas disease is a monophyletic but heterogeneous group conformed by several Discrete Typing Units (DTUs) named TcI to TcVI characterized by genetic markers. The trans-sialidase (TS) is a virulence factor involved in cell invasion and pathogenesis that is differentially expressed in aggressive and less virulent parasite stocks. Genes encoding TS-related proteins are included in a large family divided in several groups but only one of them contains TS genes. Two closely related genes differing in a T/C transition encode the enzymatically active TS (aTS) and a lectin-like TS (iTS). We quantified the aTS/iTS genes from TcII and TcVI aggressive and TcI low virulent strains and found variable aTS number (1-32) per haploid genome. In spite of being low TS enzyme-expressers, TcI strains carry 28-32 aTS gene copies. The intriguing absence of iTS genes in TcI strains together with the presence of aTS/iTS in TcII and TcVI strains (virulent) were observed. Moreover, after sequencing aTS/iTS from 38 isolates collected along the Americas encompassing all DTUs, the persistent absence of the iTS gene in TcI, TcIII and TcIV was found. In addition, the sequence clustering together with T/C transition analysis correlated to DTUs of T. cruzi. The consistence of TS results with both evolutionary genome models proposed for T. cruzi, namely the "Two Hybridization" and the "Three Ancestor" was discussed and reviewed to fit present findings. Parasite stocks to attempt genetic KO or to assay the involvement of iTS in parasite biology and virulence are finally available. |
| first_indexed | 2024-12-21T22:35:45Z |
| format | Article |
| id | doaj.art-91b67a5c0950471cbf01b5ab395a7081 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:35:45Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-91b67a5c0950471cbf01b5ab395a70812022-12-21T18:47:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5896710.1371/journal.pone.0058967Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi Discrete typing units.Juan M BurgosMarikena G RissoSimone Frédérique BrenièreChristian BarnabéOscar CampetellaMaría Susana LeguizamónTrypanosoma cruzi the agent of Chagas disease is a monophyletic but heterogeneous group conformed by several Discrete Typing Units (DTUs) named TcI to TcVI characterized by genetic markers. The trans-sialidase (TS) is a virulence factor involved in cell invasion and pathogenesis that is differentially expressed in aggressive and less virulent parasite stocks. Genes encoding TS-related proteins are included in a large family divided in several groups but only one of them contains TS genes. Two closely related genes differing in a T/C transition encode the enzymatically active TS (aTS) and a lectin-like TS (iTS). We quantified the aTS/iTS genes from TcII and TcVI aggressive and TcI low virulent strains and found variable aTS number (1-32) per haploid genome. In spite of being low TS enzyme-expressers, TcI strains carry 28-32 aTS gene copies. The intriguing absence of iTS genes in TcI strains together with the presence of aTS/iTS in TcII and TcVI strains (virulent) were observed. Moreover, after sequencing aTS/iTS from 38 isolates collected along the Americas encompassing all DTUs, the persistent absence of the iTS gene in TcI, TcIII and TcIV was found. In addition, the sequence clustering together with T/C transition analysis correlated to DTUs of T. cruzi. The consistence of TS results with both evolutionary genome models proposed for T. cruzi, namely the "Two Hybridization" and the "Three Ancestor" was discussed and reviewed to fit present findings. Parasite stocks to attempt genetic KO or to assay the involvement of iTS in parasite biology and virulence are finally available.http://europepmc.org/articles/PMC3594200?pdf=render |
| spellingShingle | Juan M Burgos Marikena G Risso Simone Frédérique Brenière Christian Barnabé Oscar Campetella María Susana Leguizamón Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi Discrete typing units. PLoS ONE |
| title | Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi Discrete typing units. |
| title_full | Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi Discrete typing units. |
| title_fullStr | Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi Discrete typing units. |
| title_full_unstemmed | Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi Discrete typing units. |
| title_short | Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi Discrete typing units. |
| title_sort | differential distribution of genes encoding the virulence factor trans sialidase along trypanosoma cruzi discrete typing units |
| url | http://europepmc.org/articles/PMC3594200?pdf=render |
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