<i>E. coli</i> CB390 as an Indicator of Total Coliphages for Microbiological Assessment of Lime and Drying Bed Treated Sludge
The use of a single host strain that allows for an evaluation of the levels of total coliphages in any type of environmental sample would facilitate the detection of and reduction in complexity and costs, favoring countries or areas with technical and economic limitations. The CB390 strain is a cand...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/13/13/1833 |
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| author | Camilo Venegas Andrea C. Sánchez-Alfonso Crispín Celis Zambrano Mauricio González Mendez Fidson-Juarismy Vesga |
| author_facet | Camilo Venegas Andrea C. Sánchez-Alfonso Crispín Celis Zambrano Mauricio González Mendez Fidson-Juarismy Vesga |
| author_sort | Camilo Venegas |
| collection | DOAJ |
| description | The use of a single host strain that allows for an evaluation of the levels of total coliphages in any type of environmental sample would facilitate the detection of and reduction in complexity and costs, favoring countries or areas with technical and economic limitations. The CB390 strain is a candidate for this type of simultaneous determinations, mainly in water samples. The objective of the study was to establish the recovery capacity of the CB390 strain in solid and semi-solid samples and to evaluate the microbiological quality of the sludge generated and stabilized by lime and drying beds in two WWTPs in Colombia. The results of both matrices indicated that CB390 recovered similar numbers of total coliphages (<i>p</i> > 0.05) against the two host strains when evaluated separately. Only the drying bed treatment was able to reduce between 2.0 and 2.9 Log<sub>10</sub> units for some microorganisms, while the addition of lime achieved a maximum reduction of 1.3 Log<sub>10</sub> units for <i>E. coli</i>. In conclusion, the CB390 strain can be used in solid and semi-solid samples, and the treatment in a drying bed provided a product of microbiological quality. However, the results are influenced by the infrastructure of the WWTP, the treatment conditions, and the monitoring of the stabilization processes. |
| first_indexed | 2024-03-10T09:52:59Z |
| format | Article |
| id | doaj.art-91b714baef124259b999d7e8ec7513d4 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-10T09:52:59Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-91b714baef124259b999d7e8ec7513d42023-11-22T02:33:44ZengMDPI AGWater2073-44412021-06-011313183310.3390/w13131833<i>E. coli</i> CB390 as an Indicator of Total Coliphages for Microbiological Assessment of Lime and Drying Bed Treated SludgeCamilo Venegas0Andrea C. Sánchez-Alfonso1Crispín Celis Zambrano2Mauricio González Mendez3Fidson-Juarismy Vesga4Department of Microbiology, School of Sciences Calidad Microbiológica de Aguas y Lodos (CMAL), Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Bogotá 110231, ColombiaDirección de Laboratorio e Innovación Ambiental, Corporación Autónoma Regional de Cundinamarca, Avenida Calle 24 (Esperanza) # 60-50, Centro Empresarial Gran Estación, Costado Esfera—Pisos 6 y 7, Bogotá 111321, ColombiaDepartment of Chemistry, Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Bogotá 110231, ColombiaSchool of Environmental and Rural Studies, Pontificia Universidad Javeriana, Transversal 4 No 42-00, Piso 8º, Bogotá 110231, ColombiaDepartment of Microbiology, School of Sciences Calidad Microbiológica de Aguas y Lodos (CMAL), Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Bogotá 110231, ColombiaThe use of a single host strain that allows for an evaluation of the levels of total coliphages in any type of environmental sample would facilitate the detection of and reduction in complexity and costs, favoring countries or areas with technical and economic limitations. The CB390 strain is a candidate for this type of simultaneous determinations, mainly in water samples. The objective of the study was to establish the recovery capacity of the CB390 strain in solid and semi-solid samples and to evaluate the microbiological quality of the sludge generated and stabilized by lime and drying beds in two WWTPs in Colombia. The results of both matrices indicated that CB390 recovered similar numbers of total coliphages (<i>p</i> > 0.05) against the two host strains when evaluated separately. Only the drying bed treatment was able to reduce between 2.0 and 2.9 Log<sub>10</sub> units for some microorganisms, while the addition of lime achieved a maximum reduction of 1.3 Log<sub>10</sub> units for <i>E. coli</i>. In conclusion, the CB390 strain can be used in solid and semi-solid samples, and the treatment in a drying bed provided a product of microbiological quality. However, the results are influenced by the infrastructure of the WWTP, the treatment conditions, and the monitoring of the stabilization processes.https://www.mdpi.com/2073-4441/13/13/1833biosolidsdomestic wastewaterheavy metalsmicrobiological indicatorssewage sludgetotal coliphages |
| spellingShingle | Camilo Venegas Andrea C. Sánchez-Alfonso Crispín Celis Zambrano Mauricio González Mendez Fidson-Juarismy Vesga <i>E. coli</i> CB390 as an Indicator of Total Coliphages for Microbiological Assessment of Lime and Drying Bed Treated Sludge Water biosolids domestic wastewater heavy metals microbiological indicators sewage sludge total coliphages |
| title | <i>E. coli</i> CB390 as an Indicator of Total Coliphages for Microbiological Assessment of Lime and Drying Bed Treated Sludge |
| title_full | <i>E. coli</i> CB390 as an Indicator of Total Coliphages for Microbiological Assessment of Lime and Drying Bed Treated Sludge |
| title_fullStr | <i>E. coli</i> CB390 as an Indicator of Total Coliphages for Microbiological Assessment of Lime and Drying Bed Treated Sludge |
| title_full_unstemmed | <i>E. coli</i> CB390 as an Indicator of Total Coliphages for Microbiological Assessment of Lime and Drying Bed Treated Sludge |
| title_short | <i>E. coli</i> CB390 as an Indicator of Total Coliphages for Microbiological Assessment of Lime and Drying Bed Treated Sludge |
| title_sort | i e coli i cb390 as an indicator of total coliphages for microbiological assessment of lime and drying bed treated sludge |
| topic | biosolids domestic wastewater heavy metals microbiological indicators sewage sludge total coliphages |
| url | https://www.mdpi.com/2073-4441/13/13/1833 |
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