Genetic variability, chemotype distribution, and aggressiveness of Fusarium culmorum on durum wheat in Tunisia
Fusarium culmorum is the most commonly reported root rot pathogen in Tunisian durum wheat. Isolates of the pathogen from four durum wheat growing areas in the north of Tunisia were analyzed for their chemotypes. Two chemotypes were detected at unequal abundance (96% of 3-ADON and 4% of NIV). Distrib...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Firenze University Press
2019-05-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5800 |
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| author | Safa OUFENSOU Virgilio BALMAS Barbara SCHERM Domenico RAU Salvatore CAMIOLO Vanda Assunta PROTA Mossadok BEN-ATTIA Samia GARGOURI Matias PASQUALI Safia EL-BOK Quirico MIGHELI |
| author_facet | Safa OUFENSOU Virgilio BALMAS Barbara SCHERM Domenico RAU Salvatore CAMIOLO Vanda Assunta PROTA Mossadok BEN-ATTIA Samia GARGOURI Matias PASQUALI Safia EL-BOK Quirico MIGHELI |
| author_sort | Safa OUFENSOU |
| collection | DOAJ |
| description | Fusarium culmorum is the most commonly reported root rot pathogen in Tunisian durum wheat. Isolates of the pathogen from four durum wheat growing areas in the north of Tunisia were analyzed for their chemotypes. Two chemotypes were detected at unequal abundance (96% of 3-ADON and 4% of NIV). Distribution of a SNP mutation located at the position 34 bp after the first exon of the EF-1α partial sequence was analysed, to verify whether the haplotype was specifically associated to Fusarium root rot. A and T haplotypes were homogeneously distributed in three different Tunisian regions (Mateur, Beja and Bousalem) but not for the region of Bizerte, from which greatest number of A haplotype strains were detected. The isolates were tested for their virulence under glasshouse conditions, and a mean of 91% of crown and root infection was observed. Chemotype influenced virulence, but there was no significant influence of the geographical origin or haplotype on virulence. The distribution of three inter simple sequence repeats (ISSR) was examined, to better understand the structure of F. culmorum populations in Tunisia. A total of 27 fragments were obtained with eight polymorphic bands. Cluster analysis showed a high level of similarity between isolates. Analysis of molecular variance confirmed that there was little genetic differentiation among F. culmorum strains from different locations. |
| first_indexed | 2024-12-14T19:06:45Z |
| format | Article |
| id | doaj.art-91bab17f6a484e61a52a844241fbe35d |
| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-12-14T19:06:45Z |
| publishDate | 2019-05-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj.art-91bab17f6a484e61a52a844241fbe35d2022-12-21T22:50:51ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952019-05-0158110.14601/Phytopathol_Mediterr-23907Genetic variability, chemotype distribution, and aggressiveness of Fusarium culmorum on durum wheat in TunisiaSafa OUFENSOU0Virgilio BALMAS1Barbara SCHERM2Domenico RAU3Salvatore CAMIOLO4Vanda Assunta PROTA5Mossadok BEN-ATTIA6Samia GARGOURI7Matias PASQUALI8Safia EL-BOK9Quirico MIGHELI10Dipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola 9, 07100 Sassari, Italy Laboratoire de Bio-surveillance de l’Environnement, Faculté des Sciences de Bizerte, Route de Tunis, 7021Zarzouna, Université de Carthage, TunisiaDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola 9, 07100 Sassari, ItalyDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola 9, 07100 Sassari, ItalyDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola 9, 07100 Sassari, ItalyDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola 9, 07100 Sassari, ItalyDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola 9, 07100 Sassari, ItalyLaboratoire de Bio-surveillance de l’Environnement, Faculté des Sciences de Bizerte, Route de Tunis, 7021Zarzouna, Université de Carthage, TunisiaLaboratoire de Protection des Végétaux, Institut National de Recherche Agronomique de Tunisie, Rue Hédi Karray, 2049 Ariana, TunisiaDeFENS-Department of Food Environmental and Nutritional Sciences, Università degli Studi di Milano, Via Celoria,2, 20133 Milano, ItalyLaboratoire de Biodiversité, Biotechnologies et Changements Climatiques, Faculté des Sciences de Tunis, Université Tunis-El Manar, 2092 Tunis, TunisiaDipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola 9, 07100 Sassari, Italy Unità di ricerca-Istituto Nazionale di Biostrutture e Biosistemi, Università degli Studi di Sassari, 07100 Sassari, ItalyFusarium culmorum is the most commonly reported root rot pathogen in Tunisian durum wheat. Isolates of the pathogen from four durum wheat growing areas in the north of Tunisia were analyzed for their chemotypes. Two chemotypes were detected at unequal abundance (96% of 3-ADON and 4% of NIV). Distribution of a SNP mutation located at the position 34 bp after the first exon of the EF-1α partial sequence was analysed, to verify whether the haplotype was specifically associated to Fusarium root rot. A and T haplotypes were homogeneously distributed in three different Tunisian regions (Mateur, Beja and Bousalem) but not for the region of Bizerte, from which greatest number of A haplotype strains were detected. The isolates were tested for their virulence under glasshouse conditions, and a mean of 91% of crown and root infection was observed. Chemotype influenced virulence, but there was no significant influence of the geographical origin or haplotype on virulence. The distribution of three inter simple sequence repeats (ISSR) was examined, to better understand the structure of F. culmorum populations in Tunisia. A total of 27 fragments were obtained with eight polymorphic bands. Cluster analysis showed a high level of similarity between isolates. Analysis of molecular variance confirmed that there was little genetic differentiation among F. culmorum strains from different locations.https://oajournals.fupress.net/index.php/pm/article/view/5800Fusarium crown and root rotspopulation structure |
| spellingShingle | Safa OUFENSOU Virgilio BALMAS Barbara SCHERM Domenico RAU Salvatore CAMIOLO Vanda Assunta PROTA Mossadok BEN-ATTIA Samia GARGOURI Matias PASQUALI Safia EL-BOK Quirico MIGHELI Genetic variability, chemotype distribution, and aggressiveness of Fusarium culmorum on durum wheat in Tunisia Phytopathologia Mediterranea Fusarium crown and root rots population structure |
| title | Genetic variability, chemotype distribution, and aggressiveness of Fusarium culmorum on durum wheat in Tunisia |
| title_full | Genetic variability, chemotype distribution, and aggressiveness of Fusarium culmorum on durum wheat in Tunisia |
| title_fullStr | Genetic variability, chemotype distribution, and aggressiveness of Fusarium culmorum on durum wheat in Tunisia |
| title_full_unstemmed | Genetic variability, chemotype distribution, and aggressiveness of Fusarium culmorum on durum wheat in Tunisia |
| title_short | Genetic variability, chemotype distribution, and aggressiveness of Fusarium culmorum on durum wheat in Tunisia |
| title_sort | genetic variability chemotype distribution and aggressiveness of fusarium culmorum on durum wheat in tunisia |
| topic | Fusarium crown and root rots population structure |
| url | https://oajournals.fupress.net/index.php/pm/article/view/5800 |
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