Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing Potential
This study aimed at the relationship between antioxidant capacity, antimicrobial activity, and in vitro evaluation of the wound healing effect of the extract obtained from Echinaceae purpureae folium (EPF). This study’s objective was to assess the bioactive components (total phenol and flavonoid con...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/15/5711 |
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| author | Cristina Burlou-Nagy Florin Bănică Rodica Anamaria Negrean Tünde Jurca Laura Grațiela Vicaș Eleonora Marian Ildikó Bácskay Rita Kiss Pálma Fehér Simona Ioana Vicaș Florina Miere (Groza) Adriana Ramona Memete Annamaria Pallag |
| author_facet | Cristina Burlou-Nagy Florin Bănică Rodica Anamaria Negrean Tünde Jurca Laura Grațiela Vicaș Eleonora Marian Ildikó Bácskay Rita Kiss Pálma Fehér Simona Ioana Vicaș Florina Miere (Groza) Adriana Ramona Memete Annamaria Pallag |
| author_sort | Cristina Burlou-Nagy |
| collection | DOAJ |
| description | This study aimed at the relationship between antioxidant capacity, antimicrobial activity, and in vitro evaluation of the wound healing effect of the extract obtained from Echinaceae purpureae folium (EPF). This study’s objective was to assess the bioactive components (total phenol and flavonoid content) and antioxidant activity of EPF extracts using the DPPH test method. The antioxidant capacity and the quantities of the compounds with antioxidant capacity were evaluated by spectrophotometric methods. Antimicrobial activity has been investigated against various pathogenic microorganisms. The minimum inhibitory concentration was determined by the microdilution method. Additionally, our work used a scratch test to examine the in vitro wound healing effects of EPF extract on NHDF cells. Statistical analysis was used to quantify the rate of migration and proliferation of fibroblast cells within the wound. Microscope pictures of fibroblast cells exposed to various EPF extract dosages were processed to estimate the width of the wound, area of the wound, and cell density inside the wound. The study proved that there was a relationship between the antioxidant, antimicrobial, and wound healing ability of EPF extracts. |
| first_indexed | 2024-03-11T00:21:43Z |
| format | Article |
| id | doaj.art-91bd0bff84a347a88c8c4f3e3fe6505c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:21:43Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-91bd0bff84a347a88c8c4f3e3fe6505c2023-11-18T23:17:53ZengMDPI AGMolecules1420-30492023-07-012815571110.3390/molecules28155711Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing PotentialCristina Burlou-Nagy0Florin Bănică1Rodica Anamaria Negrean2Tünde Jurca3Laura Grațiela Vicaș4Eleonora Marian5Ildikó Bácskay6Rita Kiss7Pálma Fehér8Simona Ioana Vicaș9Florina Miere (Groza)10Adriana Ramona Memete11Annamaria Pallag12Doctoral School of Biomedical Sciences, University of Oradea, Universitatii Street, 1, 410087 Oradea, RomaniaDepartment of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, RomaniaDepartment of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, 1st December Square 10, 410068 Oradea, RomaniaDepartment of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, RomaniaDepartment of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, RomaniaDepartment of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, RomaniaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, HungaryDepartment of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, HungaryDepartment of Food Engineering, Faculty of Environmental Protection, University of Oradea, No. 26 General Magheru Avenue, 410087 Oradea, RomaniaDepartment of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, 1st December Square 10, 410068 Oradea, RomaniaDepartment of Food Engineering, Faculty of Environmental Protection, University of Oradea, No. 26 General Magheru Avenue, 410087 Oradea, RomaniaDoctoral School of Biomedical Sciences, University of Oradea, Universitatii Street, 1, 410087 Oradea, RomaniaThis study aimed at the relationship between antioxidant capacity, antimicrobial activity, and in vitro evaluation of the wound healing effect of the extract obtained from Echinaceae purpureae folium (EPF). This study’s objective was to assess the bioactive components (total phenol and flavonoid content) and antioxidant activity of EPF extracts using the DPPH test method. The antioxidant capacity and the quantities of the compounds with antioxidant capacity were evaluated by spectrophotometric methods. Antimicrobial activity has been investigated against various pathogenic microorganisms. The minimum inhibitory concentration was determined by the microdilution method. Additionally, our work used a scratch test to examine the in vitro wound healing effects of EPF extract on NHDF cells. Statistical analysis was used to quantify the rate of migration and proliferation of fibroblast cells within the wound. Microscope pictures of fibroblast cells exposed to various EPF extract dosages were processed to estimate the width of the wound, area of the wound, and cell density inside the wound. The study proved that there was a relationship between the antioxidant, antimicrobial, and wound healing ability of EPF extracts.https://www.mdpi.com/1420-3049/28/15/5711<i>Echinacea purpurea</i> (L.) Moenchantioxidant capacityantimicrobial activitywound healing potential |
| spellingShingle | Cristina Burlou-Nagy Florin Bănică Rodica Anamaria Negrean Tünde Jurca Laura Grațiela Vicaș Eleonora Marian Ildikó Bácskay Rita Kiss Pálma Fehér Simona Ioana Vicaș Florina Miere (Groza) Adriana Ramona Memete Annamaria Pallag Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing Potential Molecules <i>Echinacea purpurea</i> (L.) Moench antioxidant capacity antimicrobial activity wound healing potential |
| title | Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing Potential |
| title_full | Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing Potential |
| title_fullStr | Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing Potential |
| title_full_unstemmed | Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing Potential |
| title_short | Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing Potential |
| title_sort | determination of the bioactive compounds from i echinacea purpurea i l moench leaves extracts in correlation with the antimicrobial activity and the in vitro wound healing potential |
| topic | <i>Echinacea purpurea</i> (L.) Moench antioxidant capacity antimicrobial activity wound healing potential |
| url | https://www.mdpi.com/1420-3049/28/15/5711 |
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