Evaluation of Diverse Phenotypic Methods for Differenting between Candida dubliniensis and Candida albicans
Candida albicans and Candida dubliniensis have a close phylogenetic relationship. The similarity between these species can cause a microbiology laboratory to identify C. dubliniensis erroneously as C. albicans. The objective of this research was to evaluate diverse phenotypic methods for differentia...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2012-01-01
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| Series: | Kasmera |
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| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/4890 |
| _version_ | 1797727653998886912 |
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| author | Dayana Camacho Sofía Mata Germán Pardi Vanessa Pineda Arantza Roselló María Teresa Collela |
| author_facet | Dayana Camacho Sofía Mata Germán Pardi Vanessa Pineda Arantza Roselló María Teresa Collela |
| author_sort | Dayana Camacho |
| collection | DOAJ |
| description | Candida albicans and Candida dubliniensis have a close phylogenetic relationship. The similarity between these species can cause a microbiology laboratory to identify C. dubliniensis erroneously as C. albicans. The objective of this research was to evaluate diverse phenotypic methods for differentiating between Candida dubliniensis and Candida albicans. Six control strains of C. dubliniensis and one of C. albicans from recognized collections were used and submitted to genotypification. Also, 70 isolates were used, identified as possible C. albicans utilizing CHROMagar Candida and bilis agar Feo mediums. The methods evaluated were: Sabouraud dextrosa agar at 45°C, Sabouraud agar with NaCl at 6.5%, Tween 80 agar, tobacco agar, Pal’s agar, tomato-carrot agar and agglutination with latex particles (Bichro-Dubli Fumouze®). It was found that the most reliable techniques for performing phenotype differentiation between these two species were tomato-carrot agar, Pal’s agar, tobacco agar and agglutination with latex particles (Bichro-Dubli Fumouze®). Of the 70 isolates considered to be C. albicans, one (1.4%) possible Candida dubliniensis was found. Nevertheless, molecular biology tests are the most appropriate means for achieving an accurate diagnosis of these two species. |
| first_indexed | 2024-03-12T11:02:39Z |
| format | Article |
| id | doaj.art-91bf7437787342e7859668b9d2b05b18 |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T11:02:39Z |
| publishDate | 2012-01-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-91bf7437787342e7859668b9d2b05b182023-09-02T04:47:57ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282012-01-01401475824369Evaluation of Diverse Phenotypic Methods for Differenting between Candida dubliniensis and Candida albicansDayana Camacho0Sofía Mata1Germán Pardi2Vanessa Pineda3Arantza Roselló4María Teresa Collela5Sección de Micología Médica, Instituto de Medicina Tropical, Universidad Central de Venezuela. Caracas, Venezuela.Sección de Micología Médica, Instituto de Medicina Tropical, Universidad Central de Venezuela. Caracas, Venezuela.Sección de Micología Médica, Instituto de Medicina Tropical, Universidad Central de Venezuela. Caracas, Venezuela.Sección de Micología Médica, Instituto de Medicina Tropical, Universidad Central de Venezuela. Caracas, Venezuela.Sección de Micología Médica, Instituto de Medicina Tropical, Universidad Central de Venezuela. Caracas, Venezuela.Sección de Micología Médica, Instituto de Medicina Tropical, Universidad Central de Venezuela. Caracas, Venezuela.Candida albicans and Candida dubliniensis have a close phylogenetic relationship. The similarity between these species can cause a microbiology laboratory to identify C. dubliniensis erroneously as C. albicans. The objective of this research was to evaluate diverse phenotypic methods for differentiating between Candida dubliniensis and Candida albicans. Six control strains of C. dubliniensis and one of C. albicans from recognized collections were used and submitted to genotypification. Also, 70 isolates were used, identified as possible C. albicans utilizing CHROMagar Candida and bilis agar Feo mediums. The methods evaluated were: Sabouraud dextrosa agar at 45°C, Sabouraud agar with NaCl at 6.5%, Tween 80 agar, tobacco agar, Pal’s agar, tomato-carrot agar and agglutination with latex particles (Bichro-Dubli Fumouze®). It was found that the most reliable techniques for performing phenotype differentiation between these two species were tomato-carrot agar, Pal’s agar, tobacco agar and agglutination with latex particles (Bichro-Dubli Fumouze®). Of the 70 isolates considered to be C. albicans, one (1.4%) possible Candida dubliniensis was found. Nevertheless, molecular biology tests are the most appropriate means for achieving an accurate diagnosis of these two species.https://produccioncientificaluz.org/index.php/kasmera/article/view/4890candida albicanscandida dubliniensisidentificación fenotípica. |
| spellingShingle | Dayana Camacho Sofía Mata Germán Pardi Vanessa Pineda Arantza Roselló María Teresa Collela Evaluation of Diverse Phenotypic Methods for Differenting between Candida dubliniensis and Candida albicans Kasmera candida albicans candida dubliniensis identificación fenotípica. |
| title | Evaluation of Diverse Phenotypic Methods for Differenting between Candida dubliniensis and Candida albicans |
| title_full | Evaluation of Diverse Phenotypic Methods for Differenting between Candida dubliniensis and Candida albicans |
| title_fullStr | Evaluation of Diverse Phenotypic Methods for Differenting between Candida dubliniensis and Candida albicans |
| title_full_unstemmed | Evaluation of Diverse Phenotypic Methods for Differenting between Candida dubliniensis and Candida albicans |
| title_short | Evaluation of Diverse Phenotypic Methods for Differenting between Candida dubliniensis and Candida albicans |
| title_sort | evaluation of diverse phenotypic methods for differenting between candida dubliniensis and candida albicans |
| topic | candida albicans candida dubliniensis identificación fenotípica. |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/4890 |
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