Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light
Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/7/2597 |
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| author | Marek Feith Tomáš Vičar Jaromír Gumulec Martina Raudenská Anette Gjörloff Wingren Michal Masařík Jan Balvan |
| author_facet | Marek Feith Tomáš Vičar Jaromír Gumulec Martina Raudenská Anette Gjörloff Wingren Michal Masařík Jan Balvan |
| author_sort | Marek Feith |
| collection | DOAJ |
| description | Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm<sup>2</sup>, 208 mJ/cm<sup>2</sup> and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm<sup>2</sup> × 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm<sup>2</sup> × 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition. |
| first_indexed | 2024-03-10T20:34:03Z |
| format | Article |
| id | doaj.art-91bfa8fc511b4234a7912d725de3b91b |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T20:34:03Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-91bfa8fc511b4234a7912d725de3b91b2023-11-19T21:09:51ZengMDPI AGApplied Sciences2076-34172020-04-01107259710.3390/app10072597Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue LightMarek Feith0Tomáš Vičar1Jaromír Gumulec2Martina Raudenská3Anette Gjörloff Wingren4Michal Masařík5Jan Balvan6Department of Physiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Physiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Physiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Physiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Biomedical Sciences, Faculty of Health and Society, Malmö University, 214 28 Malmö, SwedenDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicIncreased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm<sup>2</sup>, 208 mJ/cm<sup>2</sup> and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm<sup>2</sup> × 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm<sup>2</sup> × 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.https://www.mdpi.com/2076-3417/10/7/2597holographic microscopyquantitative phase imagingblue lightcell masscell motilitycell death |
| spellingShingle | Marek Feith Tomáš Vičar Jaromír Gumulec Martina Raudenská Anette Gjörloff Wingren Michal Masařík Jan Balvan Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light Applied Sciences holographic microscopy quantitative phase imaging blue light cell mass cell motility cell death |
| title | Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light |
| title_full | Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light |
| title_fullStr | Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light |
| title_full_unstemmed | Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light |
| title_short | Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light |
| title_sort | quantitative phase dynamics of cancer cell populations affected by blue light |
| topic | holographic microscopy quantitative phase imaging blue light cell mass cell motility cell death |
| url | https://www.mdpi.com/2076-3417/10/7/2597 |
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