Transcriptome and Physiological Analysis Highlight Lignin Metabolism of the Fruit Dots Disordering during Postharvest Cold Storage in ‘Danxiahong’ Pear
Pear (<i>Pyrus</i> L.) is one of the most important fruits in the world. Fruit dots are an important trait that affects pear quality. Abnormal fruit dots usually reduce the merchantability of pears. In this research, during cold storage, ‘Danxiahong’ pear fruit exhibited protrudent fruit...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/9/1785 |
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| author | Ruiwei Duan Xiangzhan Zhang Yudong Liu Lei Wang Jian Yang Long Wang Suke Wang Yanli Su Huabai Xue |
| author_facet | Ruiwei Duan Xiangzhan Zhang Yudong Liu Lei Wang Jian Yang Long Wang Suke Wang Yanli Su Huabai Xue |
| author_sort | Ruiwei Duan |
| collection | DOAJ |
| description | Pear (<i>Pyrus</i> L.) is one of the most important fruits in the world. Fruit dots are an important trait that affects pear quality. Abnormal fruit dots usually reduce the merchantability of pears. In this research, during cold storage, ‘Danxiahong’ pear fruit exhibited protrudent fruit dots on the peels. Microscopy system measurement showed that fruit dots size and height on the abnormal fruit peel were bigger and higher than the normal ones. Likewise, scanning electron microscopy observations indicated that the abnormal fruit peel, in contrast to the normal pear peel, exhibited an abnormal cell structure and fruit dots. Physiological analysis showed that the lignin content in abnormal fruit peel was significantly higher than in normal fruit peel. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that the top-enriched pathways were mainly associated with lignin synthesis and metabolism. The transcripts of lignin biosynthesis-associated genes were analyzed, and the results showed that the expression of a cascade of structural genes, including <i>PpyPAL</i>, <i>PpyCCR</i>, <i>PpyC3H, PpyC4H, PpyHCT</i>, <i>PpyCAD</i>, <i>PpyLAC</i>, and <i>PpyPOD</i>, was significantly induced in the protrudent peels. Furthermore, the expression of regulatory genes involved in lignin biosynthesis, especially the NAC-MYB-based gene regulatory network, was significantly upregulated in the abnormal peels. Real-time quantitative PCR (RT-qPCR) analysis confirmed the induction of lignin biosynthesis genes. Overall, this research revealed that the abnormal fruit surface was caused by fruit dots disorder during cold storage. This research provides insights into the fine regulation pathways in the prevention of fruit dots protrusion, especially in modulating lignin synthesis and metabolism during postharvest storage. |
| first_indexed | 2024-03-10T22:43:34Z |
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| language | English |
| last_indexed | 2024-03-10T22:43:34Z |
| publishDate | 2023-09-01 |
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| series | Genes |
| spelling | doaj.art-91bfe75f72e9480a81cee27ea5c1ed622023-11-19T10:53:40ZengMDPI AGGenes2073-44252023-09-01149178510.3390/genes14091785Transcriptome and Physiological Analysis Highlight Lignin Metabolism of the Fruit Dots Disordering during Postharvest Cold Storage in ‘Danxiahong’ PearRuiwei Duan0Xiangzhan Zhang1Yudong Liu2Lei Wang3Jian Yang4Long Wang5Suke Wang6Yanli Su7Huabai Xue8Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou 450009, ChinaSchool of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou 450009, ChinaPear (<i>Pyrus</i> L.) is one of the most important fruits in the world. Fruit dots are an important trait that affects pear quality. Abnormal fruit dots usually reduce the merchantability of pears. In this research, during cold storage, ‘Danxiahong’ pear fruit exhibited protrudent fruit dots on the peels. Microscopy system measurement showed that fruit dots size and height on the abnormal fruit peel were bigger and higher than the normal ones. Likewise, scanning electron microscopy observations indicated that the abnormal fruit peel, in contrast to the normal pear peel, exhibited an abnormal cell structure and fruit dots. Physiological analysis showed that the lignin content in abnormal fruit peel was significantly higher than in normal fruit peel. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that the top-enriched pathways were mainly associated with lignin synthesis and metabolism. The transcripts of lignin biosynthesis-associated genes were analyzed, and the results showed that the expression of a cascade of structural genes, including <i>PpyPAL</i>, <i>PpyCCR</i>, <i>PpyC3H, PpyC4H, PpyHCT</i>, <i>PpyCAD</i>, <i>PpyLAC</i>, and <i>PpyPOD</i>, was significantly induced in the protrudent peels. Furthermore, the expression of regulatory genes involved in lignin biosynthesis, especially the NAC-MYB-based gene regulatory network, was significantly upregulated in the abnormal peels. Real-time quantitative PCR (RT-qPCR) analysis confirmed the induction of lignin biosynthesis genes. Overall, this research revealed that the abnormal fruit surface was caused by fruit dots disorder during cold storage. This research provides insights into the fine regulation pathways in the prevention of fruit dots protrusion, especially in modulating lignin synthesis and metabolism during postharvest storage.https://www.mdpi.com/2073-4425/14/9/1785pearfruit dotsprotrusionlignin metabolismtranscriptome |
| spellingShingle | Ruiwei Duan Xiangzhan Zhang Yudong Liu Lei Wang Jian Yang Long Wang Suke Wang Yanli Su Huabai Xue Transcriptome and Physiological Analysis Highlight Lignin Metabolism of the Fruit Dots Disordering during Postharvest Cold Storage in ‘Danxiahong’ Pear Genes pear fruit dots protrusion lignin metabolism transcriptome |
| title | Transcriptome and Physiological Analysis Highlight Lignin Metabolism of the Fruit Dots Disordering during Postharvest Cold Storage in ‘Danxiahong’ Pear |
| title_full | Transcriptome and Physiological Analysis Highlight Lignin Metabolism of the Fruit Dots Disordering during Postharvest Cold Storage in ‘Danxiahong’ Pear |
| title_fullStr | Transcriptome and Physiological Analysis Highlight Lignin Metabolism of the Fruit Dots Disordering during Postharvest Cold Storage in ‘Danxiahong’ Pear |
| title_full_unstemmed | Transcriptome and Physiological Analysis Highlight Lignin Metabolism of the Fruit Dots Disordering during Postharvest Cold Storage in ‘Danxiahong’ Pear |
| title_short | Transcriptome and Physiological Analysis Highlight Lignin Metabolism of the Fruit Dots Disordering during Postharvest Cold Storage in ‘Danxiahong’ Pear |
| title_sort | transcriptome and physiological analysis highlight lignin metabolism of the fruit dots disordering during postharvest cold storage in danxiahong pear |
| topic | pear fruit dots protrusion lignin metabolism transcriptome |
| url | https://www.mdpi.com/2073-4425/14/9/1785 |
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