Comparative Genomics Analysis Provides New Insight Into Molecular Basis of Stomatal Movement in Kalanchoë fedtschenkoi
CO2 uptake and water loss in plants are regulated by microscopic pores on the surface of leaves, called stomata. This enablement of gas exchange by the opening and closing of stomata is one of the most essential processes in plant photosynthesis and transpiration, affecting water-use efficiency (WUE...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00292/full |
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| author | Robert C. Moseley Gerald A. Tuskan Gerald A. Tuskan Xiaohan Yang Xiaohan Yang Xiaohan Yang |
| author_facet | Robert C. Moseley Gerald A. Tuskan Gerald A. Tuskan Xiaohan Yang Xiaohan Yang Xiaohan Yang |
| author_sort | Robert C. Moseley |
| collection | DOAJ |
| description | CO2 uptake and water loss in plants are regulated by microscopic pores on the surface of leaves, called stomata. This enablement of gas exchange by the opening and closing of stomata is one of the most essential processes in plant photosynthesis and transpiration, affecting water-use efficiency (WUE) and thus drought susceptibility. In plant species with crassulacean acid metabolism (CAM) photosynthesis, diel stomatal movement pattern is inverted relative to C3 and C4 photosynthesis species, resulting in much higher WUE and drought tolerance. However, little is known about the molecular basis of stomatal movement in CAM species. The goal of this study is to identify candidate genes that could play a role in stomatal movement in an obligate CAM species, Kalanchoë fedtschenkoi. By way of a text-mining approach, proteins were identified in various plant species, spanning C3, C4, and CAM photosynthetic types, which are orthologous to proteins known to be involved in stomatal movement. A comparative analysis of diel time-course gene expression data was performed between K. fedtschenkoi and two C3 species (i.e., Arabidopsis thaliana and Solanum lycopersicum) to identify differential gene expression between the dusk and dawn phases of the 24-h cycle. A rescheduled catalase gene known to be involved in stomatal movement was identified, suggesting a role for H2O2 in CAM-like stomatal movement. Overall, these results provide new insights into the molecular regulation of stomatal movement in CAM plants, facilitating genetic improvement of drought resistance in agricultural crops through manipulation of stomata-related genes. |
| first_indexed | 2024-12-12T16:10:26Z |
| format | Article |
| id | doaj.art-91c376814114404f940f5ef3f7ec7f6c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T16:10:26Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-91c376814114404f940f5ef3f7ec7f6c2022-12-22T00:19:13ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-03-011010.3389/fpls.2019.00292422469Comparative Genomics Analysis Provides New Insight Into Molecular Basis of Stomatal Movement in Kalanchoë fedtschenkoiRobert C. Moseley0Gerald A. Tuskan1Gerald A. Tuskan2Xiaohan Yang3Xiaohan Yang4Xiaohan Yang5Department of Biology, Duke University, Durham, NC, United StatesBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Bredesen Center for Interdisciplinary Research and Graduate Education, The University of Tennessee, Knoxville, Knoxville, TN, United StatesCO2 uptake and water loss in plants are regulated by microscopic pores on the surface of leaves, called stomata. This enablement of gas exchange by the opening and closing of stomata is one of the most essential processes in plant photosynthesis and transpiration, affecting water-use efficiency (WUE) and thus drought susceptibility. In plant species with crassulacean acid metabolism (CAM) photosynthesis, diel stomatal movement pattern is inverted relative to C3 and C4 photosynthesis species, resulting in much higher WUE and drought tolerance. However, little is known about the molecular basis of stomatal movement in CAM species. The goal of this study is to identify candidate genes that could play a role in stomatal movement in an obligate CAM species, Kalanchoë fedtschenkoi. By way of a text-mining approach, proteins were identified in various plant species, spanning C3, C4, and CAM photosynthetic types, which are orthologous to proteins known to be involved in stomatal movement. A comparative analysis of diel time-course gene expression data was performed between K. fedtschenkoi and two C3 species (i.e., Arabidopsis thaliana and Solanum lycopersicum) to identify differential gene expression between the dusk and dawn phases of the 24-h cycle. A rescheduled catalase gene known to be involved in stomatal movement was identified, suggesting a role for H2O2 in CAM-like stomatal movement. Overall, these results provide new insights into the molecular regulation of stomatal movement in CAM plants, facilitating genetic improvement of drought resistance in agricultural crops through manipulation of stomata-related genes.https://www.frontiersin.org/article/10.3389/fpls.2019.00292/fullKalanchoë fedtschenkoistomatal movementcrassulacean acid metabolismdroughtrescheduled gene expressionArabidopsis thaliana |
| spellingShingle | Robert C. Moseley Gerald A. Tuskan Gerald A. Tuskan Xiaohan Yang Xiaohan Yang Xiaohan Yang Comparative Genomics Analysis Provides New Insight Into Molecular Basis of Stomatal Movement in Kalanchoë fedtschenkoi Frontiers in Plant Science Kalanchoë fedtschenkoi stomatal movement crassulacean acid metabolism drought rescheduled gene expression Arabidopsis thaliana |
| title | Comparative Genomics Analysis Provides New Insight Into Molecular Basis of Stomatal Movement in Kalanchoë fedtschenkoi |
| title_full | Comparative Genomics Analysis Provides New Insight Into Molecular Basis of Stomatal Movement in Kalanchoë fedtschenkoi |
| title_fullStr | Comparative Genomics Analysis Provides New Insight Into Molecular Basis of Stomatal Movement in Kalanchoë fedtschenkoi |
| title_full_unstemmed | Comparative Genomics Analysis Provides New Insight Into Molecular Basis of Stomatal Movement in Kalanchoë fedtschenkoi |
| title_short | Comparative Genomics Analysis Provides New Insight Into Molecular Basis of Stomatal Movement in Kalanchoë fedtschenkoi |
| title_sort | comparative genomics analysis provides new insight into molecular basis of stomatal movement in kalanchoe fedtschenkoi |
| topic | Kalanchoë fedtschenkoi stomatal movement crassulacean acid metabolism drought rescheduled gene expression Arabidopsis thaliana |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00292/full |
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